FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002449659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002449659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37336
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG10742.8765802442682666No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA7742.07306620955646No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA4781.280265695307478No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTCCAGGA4601.2320548532247697No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA4561.2213413327619456No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTNNAGTG4511.2079494321834154No Hit
CCTGTTTGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA4281.146346689522177No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCNAGTG3831.025819584315406No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTNCAGTG3550.9508249410756375No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA3450.9240411399185772No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNNAGTA3030.8115491750589244No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCNAGTA2930.7847653739018641No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA2800.7499464323976859No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTNAGTTGCAGTCCAGTG2590.6937004499678595No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTNNAGGA2330.6240625669595029No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTNCAGGA2020.5410327833726162No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNNAGCA1940.519605742446968No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNCAGCA1810.48478680094278975No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA1780.47675166059567176No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGCA1750.4687165202485537No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCCGATATAGATTTGTA1720.4606813799014356No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTCNAGGA1670.4472894793229055No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNNAGCA1650.44193271909149345No Hit
CCTGTTTGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCCNAGTA1620.43389757874437546No Hit
CCTGTTTGATACCCACACTTTCGAGCATNAGTGTCAGTTGCAGTNCAGTG1580.42318405828155137No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCNAGCA1560.4178272980501393No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGNGTCAGTTGCAGTNNAGTG1530.4097921577030212No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTNAGNTGCAGTCCAGTG1520.4071137775873152No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA1510.4044353974716091No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTNAGCA1500.40175701735590313No Hit
CCTGTTTGATACCNACACTTTCGAGCNTCAGNGTNAGTTGCNGTNCAGTG1460.39104349689307905No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCTAGTG1460.39104349689307905No Hit
CCTGTTTGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCNNAGTA1430.383008356545961No Hit
CCTGTTTGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCNCAGTA1420.38032997643025496No Hit
CCTGTTNGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTNCAGTG1420.38032997643025496No Hit
CCTGTTTGATACCCACACTTTNGAGCATCAGTGTCAGTTGCAGTCCAGTG1410.3776515963145489No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG1360.36425969573601885No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA1350.3615813156203128No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA1300.34818941504178275No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTNAGTTACAGTCCAGCA1290.3455110349260767No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1260.3374758945789586No Hit
CCTGTTNGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG1250.3347975144632526No Hit
CCTGTTTGANACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTNCAGTG1190.3187272337690165No Hit
CCTGTTTGATACCNACACTTTCGAGCATCAGTGTCAGTTGCAGTNCAGTG1150.3080137133061924No Hit
CNTGTTTGATACCCACACTTTCGAGCATCAGTGTNAGTTGCAGTCCAGTG1110.29730019284336834No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA1080.2892650524962503No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA1060.2839082922648382No Hit
CCTGTTTGCTCCCCACGCTTTNGAGCCTCAGCGTCAGTTATCGTCCAGTA1040.2785515320334262No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA1040.2785515320334262No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGNGTCAGTTATCGTNNAGTA1020.2731947718020141No Hit
CCTGTTTGCTACCCATGCTTTCGTGCCTCAGCGTNAGTTAAAGCCCAGTA980.26248125133919004No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA970.259802871223484No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTNAGTTAATGTCCAGGA960.257124491107778No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTTTCACCCTAGCA960.257124491107778No Hit
CCCGTTCGCTCCCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA960.257124491107778No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGTTGCAGCTTAGCA960.257124491107778No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA960.257124491107778No Hit
CCTGTTTGCNCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA960.257124491107778No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA930.24908935076065997No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA900.2410542104135419No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA890.23837583029783588No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGNTATCGTCCAGTA890.23837583029783588No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA870.23301907006642383No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAGNGTNAGTTATNGTNCAGTA850.22766230983501176No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTNAGCGTCAGTTATCGTNCAGTA820.2196271694878937No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA770.2062352689093636No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCNNAGCA750.20087850867795157No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCCGATATAGANNTGTA750.20087850867795157No Hit
CCTGTTTGANACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNCAGCA720.19284336833083351No Hit
CCTGTTNGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA710.19016498821512748No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAA710.19016498821512748No Hit
CCTGTTNGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNCAGCA700.18748660809942147No Hit
CCTGTTTGATACCNACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA690.18480822798371546No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGNGTCAGTTACAGTNNAGCA690.18480822798371546No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCNAGCA680.18212984786800943No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA650.17409470752089137No Hit
CCTGTTTGCTACCCATGCTTTCGTGCCTCAGCGTNAGNTAAAGCCCAGTA650.17409470752089137No Hit
CCCTTTNGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTCCAGGA640.17141632740518534No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTNAGNTAATGTCCAGGA640.17141632740518534No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA630.1687379472894793No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCNNAGAG630.1687379472894793No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGNTGCAGCTTAGCA620.16605956717377332No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCNCAGCA600.16070280694236128No Hit
CCTGTTTGATACCNACACTTTCGAGCNTCAANGTNAGTTGCNGCNTAGCA600.16070280694236128No Hit
CCTGTTNGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA590.15802442682665524No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTNAGNTACAGTCCAGCA580.1553460467109492No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG580.1553460467109492No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG570.1526676665952432No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGTGTCAGTTGCAGTCCAGTG570.1526676665952432No Hit
CNTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGTTGCAGCTTAGCA570.1526676665952432No Hit
CCTGTTTGATACCNACACTTTCGAGCNTCAGNGTNAGTTACNGTNCAGCA560.14998928647953716No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA560.14998928647953716No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGAA550.14731090636383115No Hit
CCTGTTTGATACCCACACTTTNGAGCATCAGCGTCAGTTACAGTCCAGCA550.14731090636383115No Hit
CCTGTTTGCNACCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCNCAGTA550.14731090636383115No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA540.14463252624812514No Hit
CCTGTTTGATACCNACACTTTCGAGCATCAGCGTCAGTTACAGTNCAGCA540.14463252624812514No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGNGTCAGTTAATGTNNAGGA540.14463252624812514No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGCCCAGTA530.1419541461324191No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCA530.1419541461324191No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA530.1419541461324191No Hit
CNCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTNAGTTAATGTCCAGGA530.1419541461324191No Hit
CCTGTTTGATACCCACACTTTCGAGCCTNAATGTCAGTTGCAGCNTAGCA520.1392757660167131No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTNAGCGTCAGTTAATGTNCAGGA520.1392757660167131No Hit
CCCTTTCGCTCCCNTGGCCTTCGTGCNTCAGNGTNAGTTAANGTNCAGGA510.13659738590100706No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCCGATATAGATNTGTA510.13659738590100706No Hit
CCCTTTCGCTCCCCTGGCCTTNGTGCCTCAGCGTCAGTTAATGTCCAGGA510.13659738590100706No Hit
CCCTTTCGCTCCCNTGGCCTTCGTGCCTCAGCGTCAGTTAATGTNCAGGA510.13659738590100706No Hit
CCTGTTNGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA510.13659738590100706No Hit
CNTGTTTGATACCCACACTTTCGAGCATCAGCGTNAGTTACAGTCCAGCA500.13391900578530105No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCCGATATAGANTTGTA500.13391900578530105No Hit
CCCGTTCGCTGCCCTGGCTTTCGCACATGAGCGTCAGTTGTGTGCCAGGA500.13391900578530105No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCNCAGAG500.13391900578530105No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAANGTCAGTTGCAGCNNAGCA490.13124062566959502No Hit
CCTGTTTGCTACCCATGCTTTNGTGCCTCAGCGTCAGTTAAAGCCCAGTA480.128562245553889No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTAAAAGCCCAGCA480.128562245553889No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA470.125883865438183No Hit
CCTGTTTGATACCCACACTTTCGAGCATNAGCGTCAGTTACAGTNCAGCA470.125883865438183No Hit
CCTGTTTGCNACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA460.12320548532247697No Hit
CCTGTTTGCTACCNATGCTTTCGTGCCTCAGCGTCAGTTAAAGCNCAGTA460.12320548532247697No Hit
CCTGTTTGATACCCACACTTTNGAGCCTCAATGTCAGTTGCAGCTTAGCA450.12052710520677094No Hit
CCTGTTTGCTACCCACACTTTNGAGCCTCAGCGTCAGTTGGTGCCCAGTA440.11784872509106492No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTNNAGCA440.11784872509106492No Hit
CCCTTTNGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTNCAGGA430.1151703449753589No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTNAGTAACGGCCCAGAG430.1151703449753589No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGCCGTCCAGTT430.1151703449753589No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGCA430.1151703449753589No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCNAGAG430.1151703449753589No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTNAGCGTCAGTTGGTGCNCAGTA420.11249196485965288No Hit
CCTGTTTGCTACCCATGCTTTCGTGCCTNAGCGTCAGTTAAAGCNCAGTA420.11249196485965288No Hit
CNTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA420.11249196485965288No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCNAGCA420.11249196485965288No Hit
CCTGTTTGANACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA420.11249196485965288No Hit
CCTGTTNGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCNCAGTA410.10981358474394685No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCNAGTA410.10981358474394685No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTNCAGTA410.10981358474394685No Hit
CCCGTTCGCTCCCCTGGCTTTCGCATCTCAGCGTCAGACACAGTNNAGAA410.10981358474394685No Hit
CCTGTTTGCTACCCATGCTTTCGTGCCTCAGNGTCAGTTAAAGCNNAGTA410.10981358474394685No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGNGTCAGTTGGTGCNNAGTA410.10981358474394685No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTNTAGTG410.10981358474394685No Hit
CCCTTTCGCNCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTNCAGGA400.10713520462824085No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCNAGCA390.10445682451253482No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTNNAGAA380.1017784443968288No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTNTGGTA380.1017784443968288No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)