FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002449661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002449661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50251
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA21664.310361982846112No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA12412.4696025949732343No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA11412.2706015800680586No Hit
CCTGTTCGATACCCGCGCTTTCGAGCTTCAGCGTCAGTTGCGCCCCAGTG8121.6158882410300293No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA7311.4546974189568367No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA7201.4328073073172674No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA7181.4288272870191638No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA5701.1343057849595033No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA4950.9850550237806214No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNNAGTA4480.8915245467751887No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCNAGTA4260.8477443234960499No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTNNAGTA4190.8338142524526875No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA4160.8278442220055322No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA4130.821874191558377No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCNAGTA3770.7502338261925136No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTNCAGTA3740.7442637957453583No Hit
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGAATCCAGACCAGA3290.6547133390380291No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA3080.6129231259079422No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA3070.6109331157588904No Hit
CCTGTTCGATACCCGCGCTTTCGAGCTTCAGCGTCAGTTGCGCCNCAGTG2990.5950130345664763No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA2780.5532228214363893No Hit
CCTGTTCGATACCCGCGCTTTCGAGCTTCAGCGTCAGTTGCGCCNNAGTG2650.5273526894987165No Hit
CCTGTTTGCTACCNACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA2620.5213826590515611No Hit
CCTGTTTGCTACCNACACTTTCGAGCNTCAGNGTNAGTTGGNGCNCAGTA2570.5114326083063023No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTNAGCGTCAGTTGGTGCNCAGTA2570.5114326083063023No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTAATCGTCCAGTA2550.5074525880081988No Hit
CNTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA2540.505462577859147No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGNTGGTGCCCAGTA2520.5014825575610435No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGNGTCAGTTGGTGCNNAGTA2520.5014825575610435No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA2510.4994925474119918No Hit
CCTGTTCGATACCCGCGCTTTCGAGCTTCAGCGTCAGTTGCGCCCNAGTG2480.4935225169648365No Hit
CCTGTTTGCTACCCACACTTTNGAGCCTCAGCGTCAGTTGGTGCCCAGTA2460.489542496666733No Hit
CCTGTTTGCNACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA2430.4835724662195777No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTACAGCTTAGCA2400.47760243577242245No Hit
CCTGTTCGATACCCGCGCTTTCGAGCTTCAGCGTNAGTTGCGCCCCAGTG2230.4437722632385425No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA2000.398002029810352No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA1840.36616186742552387No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA1780.3542218065312133No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA1690.33631171518974745No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTNNAGAA1670.33233169489164394No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA1660.3303416847425922No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAGNGTNAGTTATNGTNCAGTA1620.32238164414638515No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTNCAGAA1620.32238164414638515No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA1600.31840162384828163No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA1560.3104415832520746No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGTTAGTA1550.30845157310302285No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA1530.3044715528049193No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA1510.30049153250681576No Hit
CCTGTTTGCNCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA1510.30049153250681576No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA1500.298501522357764No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCCTCAGCGTCAGTAATCGTNCAGTA1500.298501522357764No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA1480.2945215020596605No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTNAGCGTCAGTTATCGTNCAGTA1470.29253149191060873No Hit
CCTGTTTGCTCCCCACGCTTTNGAGCCTCAGCGTCAGTTATCGTCCAGTA1470.29253149191060873No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTNCAGTA1430.2845714513144017No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGNAATCGTCCAGTA1400.27860142086724643No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA1380.2746214005691429No Hit
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGAATCCAGNNCAGA1380.2746214005691429No Hit
CCTGTTTGCNCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTNCAGTA1330.2646713498238841No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCNAGAA1310.26069132952578056No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGNGTCAGTTATCGTNNAGTA1300.2587013193767288No Hit
CCTGTTCGATACCCGCGCTTTCGAGCTTCAGCGTNAGNTGCGCCCCAGTG1300.2587013193767288No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTAATCGTCCAGTA1300.2587013193767288No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAGNGTNAGTAATNGTNCAGTA1290.25671130922767704No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGNGTCAGTAATCGTNNAGTA1280.2547212990786253No Hit
CCTGTTTGCTCCCCACGCTTTNGAGCCTCAGCGTCAGTAATCGTCCAGTA1280.2547212990786253No Hit
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGAATCCAGNCCAGA1260.25074127878052177No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTNAGCGTCAGTAATCGTNCAGTA1220.24278123818431474No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGNTATCGTCCAGTA1200.23880121788621123No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTNAGACACAGTCCAGAA1120.22288113669379717No Hit
CCTGTTCGATACCNGCGCTTTCGAGCNTCAGNGTNAGTTGCNCCNCAGTG1110.2208911265447454No Hit
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGAATCCAGANCAGA1070.21293108594853832No Hit
CCTGTTCGATACCCGCGCTTTNGAGCTTCAGCGTCAGTTGCGCCCCAGTG980.19502099460707248No Hit
CNTGTTCGATACCCGCGCTTTCGAGCTTCAGCGTNAGTTGCGCCCCAGTG960.19104097430896896No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA950.1890509641599172No Hit
CCTGTTNGATACCCGCGCTTTCGAGCTTCAGCGTCAGTTGCGCCNCAGTG930.1850709438618137No Hit
CCTGTTCGATACCNGCGCTTTCGAGCTTCAGCGTCAGTTGCGCCNCAGTG930.1850709438618137No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA890.17711090326560666No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA880.1751208931165549No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA860.17114087281845136No Hit
CCTGTTCGANACCCGCGCTTTCGAGCTTCAGCGTCAGTTGCGCCNCAGTG850.1691508626693996No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCCAGTT830.1651708423712961No Hit
CCTGTTNGATACCCGCGCTTTCGAGCTTCAGCGTCAGTTGCGCCCCAGTG830.1651708423712961No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA820.16318083222224433No Hit
CCTGTTCGATACCCGCGCTTTCGAGCTTNAGCGTCAGTTGCGCCNCAGTG790.15721080177508906No Hit
CCTGTTCGATACCCGCGCTTTCGAGCTTCAGNGTCAGTTGCGCCNNAGTG760.15124077132793376No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNNAGCA760.15124077132793376No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTACAGCNTAGCA740.14726075102983024No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTNAGCA740.14726075102983024No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT730.1452707408807785No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA690.13731070028457146No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTACAGCTNAGCA690.13731070028457146No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGCGTCAATACCGGTCCAGAC690.13731070028457146No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA660.13134066983741616No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGNGTCAGTTGGTGCNNAGTA650.1293506596883644No Hit
CCCGTTNGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA640.12736064953931264No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGCA630.12537063939026089No Hit
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGTGTNAGAATCCAGACCAGA630.12537063939026089No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTACAGCNNAGCA630.12537063939026089No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA630.12537063939026089No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA630.12537063939026089No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGNTGGTGCCCAGTA620.12338062924120913No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA610.12139061909215737No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNCAGCA580.1154205886450021No Hit
CCTGTTTGCTACCNATGCTTTCGAGCNTCAGNGTNAGTTGGNGCNCAGTA580.1154205886450021No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTNNAGCA570.11343057849595034No Hit
CCTGTTNGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA560.11144056834689858No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTNAGNCACAGTCCAGAA560.11144056834689858No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNNAGCA550.10945055819784683No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG550.10945055819784683No Hit
CCTGTTTGCTACCNATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA550.10945055819784683No Hit
CCCGTTCGCTACCCTGGCTTTNGCATCTCAGCGTCAGACACAGTCCAGAA550.10945055819784683No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNNAGTA540.10746054804879504No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCCAGTA540.10746054804879504No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTNCAGCA540.10746054804879504No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGTTGCAGCTTAGCA530.10547053789974328No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTNAGCGTCAGACACAGTNCAGAA530.10547053789974328No Hit
CCTGTTTGCNACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA530.10547053789974328No Hit
CCCGTTNGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTNCAGAA520.10348052775069153No Hit
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGNGTCAGAATCCAGNNCAGA520.10348052775069153No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTTCAGTCCAGAA510.10149051760163977No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)