FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002449811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002449811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59568
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA35145.899140478109052No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA34465.784985226967499No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTAACAGTCTAGTG30985.200778941713672No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA26904.515847434864357No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA25874.34293580445877No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA20233.396118721461187No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG15632.62389202256245No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA8791.475624496373892No Hit
CNTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA8201.3765780284716627No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA7681.2892828364222402No Hit
CNTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTAACAGTCTAGTG7251.2170964276121408No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA6771.1365162503357507No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG6671.1197287134031695No Hit
CNTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA6671.1197287134031695No Hit
CNTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA6511.0928686543110395No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA5880.9871071716357775No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA4530.7604754230459307No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA4420.7420091324200913No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA4230.7101128122481869No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGAA4200.7050765511684126No Hit
CNTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG3840.6446414182111201No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG3790.6362476497448294No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG3730.6261751275852807No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTATGCGCCAGAT3500.5875637926403438No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA3470.5825275315605694No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGCA3380.5674187483212463No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTCAGTCCAGAA2990.5019473542841794No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA2880.4834810636583401No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA2810.4717297878055332No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA2720.45662100456621No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG2390.40122213268869195No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA2350.3945071179156594No Hit
CNTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA2190.3676470588235294No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA2120.35589578297072255No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA2050.34414450711791567No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTT2010.3374294923448832No Hit
CCTGTTTGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA1950.32735697018533444No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTATGTCCCAGGA1840.30889067955949506No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA1810.30385441847972067No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT1740.2921031426269138No Hit
CNCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA1680.28203062046736505No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA1660.2786731130808488No Hit
CNTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG1630.2736368520010744No Hit
CNTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA1480.2484555466022025No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAGTGTCCAGCA1440.24174053182917005No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTGGTCGTCCAGTA1400.23502551705613753No Hit
CCTGTTTGCTACCCACACTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA1330.22327424120333067No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA1320.22159548751007255No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCA1270.2132017190437819No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTGCAGCCCAGAT1220.2048079505774913No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCGTAGCA1160.1947354284179425No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA1150.19305667472468438No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA1070.1796266451786194No Hit
NNTGTTTGCTCCCNACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA1060.17794789148536128No Hit
CNTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGAA1030.1729116304055869No Hit
CNTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG1020.17123287671232876No Hit
NNTGTTTGNTCNCCACGCTTTCGAGCCTCNGCGTCAGTTATCGTCCAGTA1000.1678753693258125No Hit
NNTGTTTGNTANCCACACTTTCGAGCCTCNATGTCAGTTGCAGCTTAGCA990.1661966156325544No Hit
NNTGTTTGATACCNACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA980.16451786193929627No Hit
CNTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA960.16116035455278002No Hit
CNTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA920.15444533977974753No Hit
NNTGTTTGNTCNCCACGCTTTCGCGCCTCNCCGTCAGTTGTCGTCCAGCA910.1527665860864894No Hit
NNTGTTTGATACCNACACTTTCGAGCATCAGTGTCAGTAACAGTCTAGTG910.1527665860864894No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTA860.14437281762019877No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTGTCAGTCCAGTA850.14269406392694062No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTCGCACCCTAGCA840.14101531023368252No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA840.14101531023368252No Hit
CCTGTTCGCTACCCACGCTCTCGTGCATCAGCGTCAGTTACAGTCTGGTA840.14101531023368252No Hit
NNTGTTTGNTANCCACACTTTCGAGCATCNGTGTCAGTAACAGTCTAGTG840.14101531023368252No Hit
NNTGTTTGNTANCCACACTTTCGAGCCTCNGCGTCAGTTGGTGCCCAGTA830.1393365565404244No Hit
CNTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA810.13597904915390813No Hit
NNTGTTTGCTACCNACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA810.13597904915390813No Hit
CNTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG810.13597904915390813No Hit
NNTGTTTGCTCCCNACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA800.13430029546065003No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGTA780.13094278807413376No Hit
CNTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTATGCGCCAGAT760.1275852806876175No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG760.1275852806876175No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA750.1259065269943594No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA700.11751275852806876No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA690.11583400483481064No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTAGTGCCCAGTA670.11247649744829438No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA660.11079774375503627No Hit
CNTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTCAGTCCAGAA630.10576148267526189No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGTCCAGTA620.10408272898200376No Hit
CNTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG620.10408272898200376No Hit
NNTGTTTGNTCNCCACGCTTTCGAGCCTCNACGTCAGTTACCGTCCAGTA620.10408272898200376No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGCA610.10240397528874565No Hit
CCCGTTCGCTACCCTGGCTTTCGCAGCTCAGCGTCAGACACAGTCCAGAA610.10240397528874565No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTCACCGTCCAGTA600.1007252215954875No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)