FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002449851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002449851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42394
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA16393.866113129216399No Hit
CCCGTTCGCTACCCTAGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA12562.9626833985941405No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA12532.955606925508327No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGCTTAGTG9602.2644713874604894No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA6471.5261593621738925No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA6291.4837005236590084No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA6261.4766240505731942No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA4881.151106288625749No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCNAGTA4851.1440298155399349No Hit
CCCGTTCGCTACCCTAGCTTTCGAGCCTCAGCGTCAGTTACAGTNNAGAA4841.1416709911779968No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNNAGTA4831.1393121668160588No Hit
CCCGTTCGCTACCCTAGCTTTCGAGCCTCAGCGTCAGTTACAGTCNAGAA4751.1204415719205547No Hit
CCCGTTCGCTACCCTAGCTTTCGAGCCTCAGCGTCAGTTACAGTNCAGAA4631.0921356795772987No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGCNNAGTG3890.9175826767938859No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA3840.9057885549841959No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGCNTAGTG3700.8727650139170637No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGCTNAGTG3480.8208708779544275No Hit
CCCGTTCGCTACCCTAGCTTTCGAGCCTCAGCGTNAGTTACAGTCCAGAA2990.7052884842194651No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACAGTTTAGCA2890.6817002406000849No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA2880.6793414162381469No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA2820.6651884700665188No Hit
CCTGTTTGCTACCNACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA2240.5283766570741142No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGNTGGTGCCCAGTA2230.5260178327121763No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTNAGTTACGGCTTAGTG2190.5165825352644242No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGNGTCAGTTGGTGCNNAGTA2160.5095060621786102No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTNAGCGTCAGTTGGTGCNCAGTA2120.5000707647308582No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA2100.49535311600698206No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA2070.4882766429211681No Hit
CCTGTTTGCTACCNACACTTTCGAGCNTCAGNGTNAGTTGGNGCNCAGTA1940.4576119262159739No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1890.44581780440628394No Hit
CCTGTTTGCTACCCACACTTTNGAGCCTCAGCGTCAGTTGGTGCCCAGTA1870.44110015568240785No Hit
CNTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA1820.4293060338727179No Hit
CCTGTTTGCNACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1800.4245883851488418No Hit
CCCGTTCGCTACCNTAGCTTTCGAGCCTCAGCGTCAGTTACAGTNCAGAA1800.4245883851488418No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA1780.41987073642496575No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1770.4175119120630278No Hit
CCCGTTCGCTACCCTAGCTTTCGAGCCTCAGCGTNAGNTACAGTCCAGAA1660.3915648440817097No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGNTATCGTCCAGTA1630.38448837099589567No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA1620.38212954663395765No Hit
CCCGTTNGCTACCCTAGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA1590.3750530735481436No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA1580.3726942491862056No Hit
CNCGTTCGCTACCCTAGCTTTCGAGCCTCAGCGTNAGTTACAGTCCAGAA1550.3656177761003916No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAGNGTNAGTTATNGTNCAGTA1530.36090012737651556No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGNGTCAGTTATCGTNNAGTA1510.3561824786526395No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA1490.3514648299287635No Hit
CCTGTTTGCNCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA1450.3420295324810115No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTNAGCGTCAGTTATCGTNCAGTA1440.33967070811907346No Hit
CCCGTTNGCTACCCTAGCTTTCGAGCCTCAGCGTCAGTTACAGTNCAGAA1430.33731188375713544No Hit
CCTGTTTGCTCCCCACGCTTTNGAGCCTCAGCGTCAGTTATCGTCCAGTA1420.33495305939519743No Hit
CCCGTTCGCTACCCTAGCTTTCGAGCCTCAGNGTCAGTTACAGTNNAGAA1350.31844128886163137No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT1350.31844128886163137No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA1340.31608246449969335No Hit
CCTGTTTGATACCCACACTTTCGAGCATNAACGTCAGTTACGGCNTAGTG1320.3113648157758173No Hit
CCCGTTCGCTACCNTAGCTTTCGAGCNTCAGNGTNAGTTACNGTNCAGAA1310.30900599141387936No Hit
CCCGTTCGCTACCCTCGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA1290.30428834269000327No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAANGTCAGTTACGGCNNAGTG1290.30428834269000327No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA1280.30192951832806525No Hit
CCCGTTCGCNACCCTAGCTTTCGAGCCTCAGCGTCAGTTACAGTNCAGAA1250.29485304524225125No Hit
CCCGTTCGCTACCCTAGCTTTNGAGCCTCAGCGTCAGTTACAGTCCAGAA1250.29485304524225125No Hit
CCCGTTCGCTACCCTAGCTTTCGAGCCTNAGCGTCAGTTACAGTNCAGAA1220.28777657215643726No Hit
CCTGTTTGATACCNACACTTTCGAGCNTGAANGTNAGTTACNGCNTAGTG1200.28305892343256117No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTNAGNTACGGCTTAGTG1170.2759824503467472No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCNAGAA1150.2712648016228712No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACAGTNNAGCA1110.26182950417511913No Hit
CCTGTTTGATACCNACACTTTCGAGCATGAACGTCAGTTACGGCNTAGTG1100.2594706798131811No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACAGTTNAGCA1090.2571118554512431No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTNCAGAA1080.2547530310893051No Hit
CNTGTTTGATACCCACACTTTCGAGCATGAACGTNAGTTACGGCTTAGTG1060.2500353823654291No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACAGTNTAGCA1040.24531773364155304No Hit
CCTGTTNGATACCCACACTTTCGAGCATGAACGTCAGTTACGGCNTAGTG1030.24295890927961503No Hit
CCTGTTNGATACCCACACTTTCGAGCATGAACGTCAGTTACGGCTTAGTG1000.23588243619380103No Hit
CCTGTTTGATACCCACACTTTNGAGCATGAACGTCAGTTACGGCTTAGTG980.231164787469925No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTNNAGAA960.22644713874604896No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCNAGTA960.22644713874604896No Hit
CCTGTTTGANACCCACACTTTCGAGCATGAACGTCAGTTACGGCNTAGTG950.22408831438411095No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNCAGTA900.2122941925744209No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA900.2122941925744209No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA900.2122941925744209No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA860.20285889512666888No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA830.19578242204085486No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA800.1887059489550408No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTNAGACACAGTCCAGAA800.1887059489550408No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNNAGTA780.1839883002311648No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTNAGCA780.1839883002311648No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA770.1816294758692268No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNNAGCA730.17219417842147475No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGCA730.17219417842147475No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNCAGCA710.16747652969759871No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTNAGTTACAGTTTAGCA710.16747652969759871No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA690.1627588809737227No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA660.15568240788790866No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCNAGCA630.14860593480209464No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTNCAGTA630.14860593480209464No Hit
CCCGTTTGCTACCCTAGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA630.14860593480209464No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA600.14152946171628059No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCNAGTA590.1391706373543426No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNNAGCA580.13681181299240458No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGTTACCGTCCAGTA570.1344529886304666No Hit
CCCGTTCGCTACCCTAGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGCA560.13209416426852855No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGNGTCAGACACAGTNNAGAA540.12737651554465254No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG530.12501769118271455No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGTTGCAGCTTAGCA530.12501769118271455No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCNCAGTT530.12501769118271455No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAANGTNAGTTACNGTNCAGTA510.12030004245883852No Hit
CCCGTTCGCTACCCTCGCTTTCGAGCCTCAGCGTCAGTTACAGTNNAGAA490.1155823937349625No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA490.1155823937349625No Hit
CCTGTTTGATACCNACACTTTCGAGCATGAACGTCAGTTACAGTNTAGCA480.11322356937302448No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTNAGTTACAGTCCAGCA470.11086474501108648No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTNNAGTA460.10850592064914846No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCNAGCA450.10614709628721045No Hit
CNCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTNAGACACAGTCCAGAA450.10614709628721045No Hit
CCCGTTNGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTNCAGAA430.10142944756333444No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)