FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002450045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002450045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33274
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA4351.3073270421350003No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA3090.9286530023441726No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACAGTCCAGTA3010.9046102061669772No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA2880.8655406623790347No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA2370.712267836749414No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA2310.6942357396165174No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCA2030.6100859529963335No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA1990.5980645549077358No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTA1610.48386127306605753No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAA1560.46883452545531046No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA1520.45681312736671276No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA1480.444791729278115No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1450.4357756807116668No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG1450.4357756807116668No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGCA1360.4087275350123219No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAACGTCAGTGGCGGCTTAGCA1350.40572218549017247No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACAGTCNAGTA1350.40572218549017247No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA1320.39670613692372425No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA1260.3786740397908277No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNNAGCA1180.35463124361363224No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCNAGTA1160.34862054456933345No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTT1150.34561519504718397No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACAGTNCAGTA1110.33359379695858626No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTCCAGGA1080.324577748392138No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACAGTNNAGTA1060.3185670493478392No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTNNAGTA1050.3155616998256897No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA1050.3155616998256897No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTNAGCA1030.3095510007813909No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTT1010.303540301737092No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTNCAGTA1000.30053495221494264No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTNCAGCA980.29452425317064374No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCNAGCA960.2885135541263449No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNNAGTA940.28250285508204603No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGTA910.27348680651559776No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG910.27348680651559776No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNCAGCA910.27348680651559776No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCNAGTA910.27348680651559776No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCNAGCA900.27048145699344833No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNNAGCA900.27048145699344833No Hit
CCTGTTTGATACCCGCACCTTCGAGCCTCAGCGTCAGTCGGCCACCGGGC890.2674761074712989No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG860.25846005890485063No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTAGTGCCCAGTA850.2554547093827012No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTNNAGCA840.2524493598605518No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGTTGCAGCTTAGCA840.2524493598605518No Hit
CCTGTTTGATACCCGCACTTTCGAGCATCAACGTCAGTTACGGTCCAGCA840.2524493598605518No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA760.22840656368335638No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA750.22540121416120695No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTNAGTTACAGTCCAGTA750.22540121416120695No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCNCAGCA750.22540121416120695No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA710.21337981607260922No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA700.21037446655045983No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA680.20436376750616095No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGTTACTGTCCAGTA670.20135841798401155No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG660.19835306846186213No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCNAGCA650.19534771893971267No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTTATGGCTTGGCA650.19534771893971267No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCNNAGTA640.19234236941756327No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTNCAGAA610.183326320851115No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCNNAGAG600.18032097132896555No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGAA600.18032097132896555No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGTTGTCGTCCAGCA600.18032097132896555No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCNAGTA590.17731562180681612No Hit
CCTGTTTGCTACCCACACTTTNGAGCCTCAGCGTCAGTTGGTGCCCAGTA570.17130492276251727No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCNCAGAG570.17130492276251727No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTNAGTTGTCGTCCAGCA570.17130492276251727No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTNNAGCA560.16829957324036784No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGNGTCAGTTGGTGCNNAGTA560.16829957324036784No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA560.16829957324036784No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA560.16829957324036784No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCNCAGTA550.16529422371821845No Hit
CCTGTTTGCTACCNACACTTTCGAGCNTCAGNGTNAGTTGGNGCNCAGTA550.16529422371821845No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA540.162288874196069No Hit
CCTGTTTGCTACCNACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA540.162288874196069No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCATGTCCCAGAG530.1592835246739196No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTNNAGGA530.1592835246739196No Hit
CCTGTTTGATACCCACACTTTNGAGCCTCAATGTCAGTTGCAGCTTAGCA530.1592835246739196No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGCCCAGTA510.15327282562962072No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCNAGCA510.15327282562962072No Hit
CCTGTTTGATACCCGCACTTTCGAGCATCAGCGTCAGTTATGCTACAGTA510.15327282562962072No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGNTGCAGCTTAGCA510.15327282562962072No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAACGTCAGTGGCGGCNNAGCA510.15327282562962072No Hit
CCTGTTCGATACCCACGCTTTCGAGCTTCAGCGTCAGTTGCGCTACAGCA510.15327282562962072No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA510.15327282562962072No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCNNAGCA510.15327282562962072No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA500.15026747610747132No Hit
CCTGTTTGATACCCACACTTTCGAGCCTNAATGTCAGTTGCAGCNTAGCA490.14726212658532187No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAANGTNAGTTACNGTNCAGTA490.14726212658532187No Hit
CCTGTTTGATACCNACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA490.14726212658532187No Hit
CNTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA490.14726212658532187No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTNAGTGACGGCCCAGAG490.14726212658532187No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTNNAGTA480.14425677706317244No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG460.1382460780188736No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAACGTCAGTGGCGGCNTAGCA460.1382460780188736No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTGCAGTCCAGAA450.13524072849672417No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTNNAGAA450.13524072849672417No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTNCAGGA450.13524072849672417No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTAAAAGCNNAGTT450.13524072849672417No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCNAGAA450.13524072849672417No Hit
CCTGTTNGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA450.13524072849672417No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGNTGGTGCCCAGTA440.13223537897457474No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTNCAGCA440.13223537897457474No Hit
CCTATTTGCTCCCCACGCTTTCGGGAATGAGCGTCAGTTGCGGCCCAGAC440.13223537897457474No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTNAGCGTCAGTTGGTGCNCAGTA440.13223537897457474No Hit
CCTGTTNGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA440.13223537897457474No Hit
CCTGTTTGCNCCCCACACTTTCGTGCCTCAACGTCAGTTACAGTNCAGTA440.13223537897457474No Hit
CCTGTTTGCNACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA430.12923002945242532No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNCAGTA430.12923002945242532No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCNAGAG430.12923002945242532No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTAGTGCNCAGTA430.12923002945242532No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAANGTCAGTTGCAGCNNAGCA420.1262246799302759No Hit
CCTGTTTGCTCCCCACACTTTNGTGCCTCAACGTCAGTTACAGTCCAGTA420.1262246799302759No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGTTACTGTCCAGTA410.12321933040812647No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTNAGTTAGAGCCCAGCA410.12321933040812647No Hit
CCTGTTTGCTCCCNACACTTTCGTGCCTCAACGTCAGTTACAGTNCAGTA410.12321933040812647No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAACGTCAGTGGCGGCTNAGCA410.12321933040812647No Hit
CNTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGTTGCAGCTTAGCA410.12321933040812647No Hit
CCTGTTTGANACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA410.12321933040812647No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA410.12321933040812647No Hit
CCTGTTTGCNCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTNCAGTA400.12021398088597704No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTNAGNTACAGTCCAGTA400.12021398088597704No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTNCAGTA390.11720863136382761No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTNCAGTA390.11720863136382761No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTAAAAGCCCAGTA390.11720863136382761No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTAGTGCNNAGTA390.11720863136382761No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG390.11720863136382761No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCNAGTA380.11420328184167819No Hit
CCTGTTTGATACCNACACTTTCGAGCNTCAANGTNAGTTGCNGCNTAGCA380.11420328184167819No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTNNAGTT380.11420328184167819No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTNNAGGG370.11119793231952875No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTAGTGCCNAGTA370.11119793231952875No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA370.11119793231952875No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA370.11119793231952875No Hit
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGAATCCAGACCAGA370.11119793231952875No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTCNAGGA360.10819258279737934No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAANGTCAGTTACTGTNNAGTA360.10819258279737934No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAANGTCAGTTACAGTNNAGTA350.10518723327522991No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTTCTGTCCAGTG350.10518723327522991No Hit
CCTGTTTGATACCCGCACCTTCGAGCCTCAGCGTCAGTCGGCCANCGGGC350.10518723327522991No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA350.10518723327522991No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTNCAGTT350.10518723327522991No Hit
CCTGTTTGCTCCCNACGCTTTCGCGCCTCACCGTCAGTTGTCGTNCAGCA340.10218188375308047No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNNAGTA340.10218188375308047No Hit
CCTGTTTGCTCCCNACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNCAGCA340.10218188375308047No Hit
CNTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGTTGTCGTCCAGCA340.10218188375308047No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT340.10218188375308047No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTAAAAGCNCAGTT340.10218188375308047No Hit
CCTGTTNGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA340.10218188375308047No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)