FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002450131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002450131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85022
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA84619.95154195384724No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA45775.383312554397685No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA39574.654089529768766No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGAA33583.949565994683729No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA26983.1732963233045566No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA23072.7134153513208346No Hit
CNTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA21842.568746912563807No Hit
CCCGTTCGCTCCCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA17702.0818141186986896No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG11411.3420055985509634No Hit
CNTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA10501.234974477194138No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGCTTAGTG10381.2208604831690621No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTCCGGTA10171.1961609936251794No Hit
CNTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA9981.1738138364188093No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTACAGCTTAGCA9771.1491143468749265No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA9741.1455858483686574No Hit
CNTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGAA7600.8938862882548045No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA7200.8468396415045518No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA6480.7621556773540965No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA5780.679824045541154No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT5330.6268965679471196No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTGCTGTCCAGCA4880.5739690903530851No Hit
CNCGTTCGCTCCCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA4680.5504457669779587No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG4300.5057514525652184No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA4180.49163745854014257No Hit
CCTGTTCGATACCCGCGCCTTCGAGCATCAGCGTCAGTGTGCAACTGGCA3900.45870480581496553No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTCGCACCCTAGCA3660.4304768177648138No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA3630.4269483192585449No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG3440.4046011620521747No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA3400.3998964973771495No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTGCTGCCCCGGCA3330.3916633341958552No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA3290.3869586695208299No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA2890.339912022770577No Hit
NNTGTTTGCTACCNACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA2820.3316788595892828No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA2700.3175648655642069No Hit
CNTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTCCGGTA2700.3175648655642069No Hit
CNTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG2690.31638869939545056No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG2640.31050786855166895No Hit
CNTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA2550.2999223730328621No Hit
CNTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTACAGCTTAGCA2460.2893368775140552No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG2450.28816071134529886No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA2420.2846322128390299No Hit
NNTGTTTGNTANCCACACTTTCGAGCCTCNGCGTCAGTTGGTGCCCAGTA2410.2834560466702736No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGCCGTCCAGTT2390.28110371433276093No Hit
CNTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGCTTAGTG2290.2693420526451977No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTCATCGTCCAGTA1950.22935240290748277No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGTCCAGTA1930.22700007056997012No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA1890.22229540589494481No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA1870.2199430735574322No Hit
CNTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA1750.20582907953235635No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA1690.19877208251981843No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA1650.19406741784479312No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA1590.18701042083225516No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA1530.17995342381971724No Hit
CNTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTGCTGTCCAGCA1480.17407259297593564No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA1480.17407259297593564No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGTA1460.171720260638423No Hit
NNTGTTTGNTCNCCACGCTTTCGTGCCTCNGTGTCAGTAGCAGTCCAGTA1400.16466326362588507No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTTCCCAGTA1360.1599585989508598No Hit
ACTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1360.1599585989508598No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCA1330.15643010044459082No Hit
CNTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT1320.15525393427583448No Hit
NNTGTTTGCTCCCNACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA1280.15054926960080922No Hit
CCCGTTCGCTCCCCTGGCTTTCGCAGCTCAGCGTCAGACACAGTCCAGAA1270.14937310343205287No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGTTAGTA1250.14702077109454023No Hit
CCTGTTTGCTACCCACACTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA1240.1458446049257839No Hit
CCTGTTTGCTACCCACACTTTCGCGCCTCAGCGTCAGTTGGTGCCCAGTA1200.14113994025075863No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTAACAGTCCAGTA1200.14113994025075863No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGAA1150.13525910940697702No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGCA1140.1340829432382207No Hit
NNTGTTTGNTCNCCACGCTTTCGAGCCTCNGCGTCAGTTATCGTCCAGTA1130.13290677706946438No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA1110.1305544447319517No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTTATGCCCCGGCA1100.12937827856319542No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCCAGTA1090.1282021123944391No Hit
NNTGTTTGCTACCNATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1080.12702594622568275No Hit
NNTGTTTGNTANCCATGCTTTCGAGCCTCNGCGTCAGTTGGTGCCCAGTA1080.12702594622568275No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGCA1080.12702594622568275No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCTAGTA1070.12584978005692643No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCTAGAA1030.12114511538190115No Hit
CNTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA1000.11761661687563219No Hit
NNTGTTTGNTCNCCACACTTTCGTGCCTCNACGTCAGTTACTGTCCAGAA990.11644045070687586No Hit
CCTGTTCGATACCCGCACTTTCGAGCGTCAGCGTCAGTTGCGCTCCAGTG970.11408811836936322No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCTCAGTA960.1129119522006069No Hit
CNTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG930.10938345369433794No Hit
CCTATTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAACGGCCCAGCA930.10938345369433794No Hit
CCTGTTTGCTCCCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA860.10115029051304368No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)