FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002450180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002450180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50459
Sequences flagged as poor quality0
Sequence length301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGAATATTGGGCAATGGGCGCAAGCCTGACCCAGCAACGCCGCGTGA908318.00075308666442No Hit
TGAGGAATATTGGTCAATGGACGCAAGTCTGAACCAGCCATGCCGCGTGC37577.44564894270596No Hit
TGGGGGATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGG37517.433758100636161No Hit
TGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGA27805.509423492340316No Hit
TGAGGAATATTGGTCAATGGACGAGAGTCTGAACCAGCCAAGTAGCGTGA26965.342951703363127No Hit
TGAGGAATATTGGTCAATGGGCGAGAGCCTGAACCAGCCAAGTAGCGTGA22804.518519986523712No Hit
TGGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCAACGCCGCGTGA15603.0916189381478034No Hit
TGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCAACGCCGCGTGA14662.905329079054282No Hit
TGGGGAATCTTCCGCAATGGGCGAAAGCCTGACGGAGCAACGCCGCGTGA13762.726966448007293No Hit
TGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGG11442.267187221308389No Hit
TGAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCCAAGTAGCGTGA11102.19980578291286No Hit
TCGAGAATCATTCACAATGGGGGAAACCCTGATGGTGTGACGCCGCGTGG9881.9580253274936086No Hit
TGGGGAATATTGGGCAATGGGGGAAACCCTGACCCAGCAACGCCGCGTGA9271.837135099783983No Hit
TGAGGAATATTGGTCAATGGACGGAAGTCTGAACCAGCCATGCCGCGTGC9141.8113716086327514No Hit
TGAGGAATATTGGTCAATGGGCGCGAGCCTGAACCAGCCAAGTAGCGTGC8681.7202084860976237No Hit
TGGGGGATATTGGACAATGGGGGCAACCCTGATCCAGCGACGCCGCGTGA8561.696426801958025No Hit
TGGGGGATATTGCGCAATGGGGGCAACCCTGACGCAGCAACGCCGCGTGA8451.67462692483006No Hit
TTAGGAATATTCGTCAATGGGGGAAACCCTGAACGAGCAATGCCGCGTGA8381.6607542757486276No Hit
TGAGGAATATTGGTCAATGGGCGTAAGCCTGAACCAGCCAAGTAGCGTGA7361.4586099605620404No Hit
TGAGGAATATTGGTCAATGGTCGGCAGACTGAACCAGCCAAGTCGCGTGA7221.4308646623991756No Hit
TGGGGAATATTGCACAATGGAGGAAACTCTGATGCAGCGACGCCGCGTGA5291.0483759091539666No Hit
TGAGGAATATTGGTCAATGGGCGGAAGCCTGAACCAGCCAAGTAGCGTGA5141.0186488039794686No Hit
TGGGGAATATTGGGCAATGGAGGAAACTCTGACCCAGCAACGCCGCGTGG5071.004776154898036No Hit
TGAGGAATATTGGTCAATGGACGCGAGTCTGAACCAGCCAAGTAGCGTGA4860.9631582076537387No Hit
TGGGGAATATTGCACAATGGAGGAAACTCTGATGCAGCGATGCCGCGTGA4610.9136130323629085No Hit
TGGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCGACGCCGCGTGA4550.9017221902931093No Hit
TGGGGGATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGA4030.7986682256881825No Hit
TGAGGAATATTGGTCAATGGGCGGGAGCCTGAACCAGCCAAGTAGCGTGA4030.7986682256881825No Hit
TGAGGAATATTGGTCAATGGCCGAGAGGCTGAACCAGCCAAGTCGCGTGA2920.5786876473968965No Hit
TGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCAACGCCGCGTGA2880.5707604193503637No Hit
TAGGGAATATTGGGCAATGGGCGCAAGCCTGACCCAGCAACGCCGCGTGA2730.5410333141758655No Hit
TGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA2500.4954517529083018No Hit
TGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGA2230.44194296359420526No Hit
TAGGGAATCTTCGGCAATGGGGGCAACCCTGACCGAGCAACGCCGCGTGA1900.3765433322103094No Hit
TGAGGAATATTGGTCAATGGGCGGAAGCCTGAACCAGCCATGCCGCGTGA1800.3567252620939773No Hit
TAGGGAATATTGGACAATGGGGGAAACCCTGATCCAGCAACGCCGCGTGA1770.35077984105907767No Hit
TGGGGAATATTGGGCAATGGAGGCAACTCTGACCCAGCAATGCCGCGTGA1760.34879803404744447No Hit
TGGGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGA1590.31510731484967996No Hit
TGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCGACGCCGCGTGA1510.2992528587566143No Hit
TGAGGGATATTGGGCAATGGGGGAAACCCTGACCCAGCAACGCCGCGTGA1510.2992528587566143No Hit
TGGGGAATATTGGGCAATGGGGGGAACCCTGACCCAGCAACGCCGCGTGG1390.27547117461701576No Hit
TGGGGAATATTGGGCAATGGGCGAAAGCCTTACCCAGCAACGCCGCGTGA1360.26952575358211617No Hit
TGGGGAATATTGCACAATGGGGGGAACCCTGATGCAGCGACGCCGCGTGA1360.26952575358211617No Hit
TGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCGACGCCGCGTGC1260.24970768346578412No Hit
TGGGGAATATTGGGCAATGGGGGGAACCCTGACCCAGCAACGCCGCGTGA1230.24376226243088445No Hit
TGGGGAATATTGGGCAATGGACGAAAGTCTGACCCAGCGACGCCGCGTGA1210.23979864840761805No Hit
TGGGGAATCTTGCGCAATGGGGGGAACCCTGACGCAGCGACGCCGCGTGC1050.20808973622148674No Hit
TAGGGAATTTTCGGCAATGGGGGAAACCCTGACCGAGCAACGCCGCGTGA1000.19818070116332073No Hit
TGAGGGATATTGGGCAATGGGGGAAACCCTGACCCAGCGACGCCGCGTGA970.1922352801284211No Hit
TGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGATGCCGCGTGA840.1664717889771894No Hit
TGGGGAATATTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA720.14269010483759093No Hit
TGAGGAATATTGGTCAATGGACGAGAGTCTGAACCAGCCAAGTCGCGTGA700.1387264908143245No Hit
TGAGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCCATGCCGCGTGC700.1387264908143245No Hit
TGGGGAATATTGGGCAATGGGGGCAACCCTGACCCAGCAACGCCGCGTGA690.13674468380269128No Hit
TCGAGAATCATTCACAATGGGGGAAACCCTGATGGGGGGACGCCGCGTGG630.12485384173289206No Hit
TGGGGGATATTGCACAATGGGGGAAACCCTGATGCAGCAACGCCGCGTGA620.12287203472125885No Hit
TGGGGGATATTGCACAATGGAGGAAACTCTGATGCAGCGACGCCGCGTGA590.11692661368635923No Hit
TGAGGAATATTGGTCAATGGACGTAAGTCTGAACCAGCCAAGTCGCGTGA570.1129629996630928No Hit
TGGGGAATTTTGCGCAATGGGGGCAACCCTGACGCAGCAACGCCGCGTGC540.1070175786281932No Hit
TGAGGAATATTGGTCAATGGGCGATGGCCTGAACCAGCCAAGTAGCGTGA510.10107215759329356No Hit
TGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGT510.10107215759329356No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)