FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002450189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002450189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45446
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG812317.873960304537253No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA27165.97632354882718No Hit
CNTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG18784.132376886854729No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA11742.583285657703648No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA9632.1189983716938787No Hit
CCTGTTCGATACCCGCACTTTCGAGCGTCAGCGTCAGTTGCGCTCCAGTG7081.557892883862166No Hit
CNTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA6861.509483782951195No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGTTAGTA5001.1002068388857105No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA5001.1002068388857105No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGAA4621.0165911191303965No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA4330.9527791224750254No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCCAGTT3970.8735642300752541No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA3840.8449588522642257No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGAA3630.7987501650310258No Hit
CNTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA2680.5897108656427408No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCGCTCCAGTG2450.5391013510539981No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA2420.5325001100206839No Hit
NNTGTTCGATACCNGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG2420.5325001100206839No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA2390.5258988689873696No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG2290.5038947322096554No Hit
NNTGTTCGNTANCCGCACTTTCGAGCTTCNGCGTCAGTTGCGCTCCAGTG2290.5038947322096554No Hit
CNTGTTCGATACCCGCACTTTCGAGCGTCAGCGTCAGTTGCGCTCCAGTG2180.4796901817541698No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA2040.44888439026536986No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA2030.44668397658759845No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA1970.43348149452096996No Hit
CCTGTTCGATACCCGCACTTTCGCGCTTCAGCGTCAGTTGCGCTCCAGTG1900.41807859877657No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGCA1800.39607446199885576No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA1680.3696694978655987No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA1660.36526867051005585No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA1630.35866742947674163No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCGCATCCCAGGG1590.34986577476565595No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTTATGCCCCGGCA1570.3454649474101131No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA1490.3278616379879417No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA1450.31905998327685603No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA1290.2838533644325133No Hit
CCTGTTCGCTACCCACGCTCTCGTGCATCAGCGTCAGTTACAGTCTGGTA1190.2618492276547991No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCTAGTG1160.25524798662148485No Hit
CNTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGTTAGTA1150.25304757294371344No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTGTCGTCCAGTA1090.2398450908770849No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA1090.2398450908770849No Hit
CNTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGAA940.20683888571051356No Hit
CNTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA930.20463847203274216No Hit
CNTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGAA910.20023764467719932No Hit
NNTGTTTGNTANCCACACTTTCGAGCCTCNGCGTCAGTTGGTGCCCAGTA900.19803723099942788No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCTCTCCAGTG890.19583681732165648No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTACCGTCCAGTA860.1892355762883422No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTATTGTCCAGAA850.18703516261057077No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTTCGCTCCAGTG840.18483474893279936No Hit
CNTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA800.17603309422171368No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG800.17603309422171368No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTTTCACCCTAGCA790.17383268054394224No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGG790.17383268054394224No Hit
CNTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCCAGTT770.1694318531883994No Hit
NNTGTTTGCTACCNACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA770.1694318531883994No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCCAGTA720.1584297847995423No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGTCCAGTA720.1584297847995423No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTG700.15402895744399947No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCCGCGTCAGTTGCGCTCCAGTG670.1474277164106852No Hit
CNTGTTCGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCGCTCCAGTG670.1474277164106852No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG670.1474277164106852No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCCGTTGCGCTCCAGTG660.1452273027329138No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG650.14302688905514235No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTA630.1386260616995995No Hit
CCTGTTTGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG610.13422523434405667No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTA610.13422523434405667No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCTAGTG610.13422523434405667No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTTCCCAGTA570.125423579632971No Hit
CNTGTTCGATACCCGCACTTTCGCGCTTCAGCGTCAGTTGCGCTCCAGTG560.12322316595519958No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTCAGTCCAGAA560.12322316595519958No Hit
CCTGTTTGCTACCCACACTTTCGCGCCTCAGCGTCAGTTGGTGCCCAGTA560.12322316595519958No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA550.12102275227742815No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCCCCAGTG550.12102275227742815No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTTCAGTG520.1144215112441139No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTT520.1144215112441139No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACAGTCCAGTA520.1144215112441139No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCGTAGCA510.11222109756634246No Hit
CNTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA510.11222109756634246No Hit
CNTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGCA490.10782027021079962No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGTA480.10561985653302822No Hit
CCTGTTCGATACCCGCACTTTCGTGCTTCAGCGTCAGTTGCGCTCCAGTG480.10561985653302822No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA480.10561985653302822No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA470.10341944285525678No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA460.10121902917748536No Hit
CNTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA460.10121902917748536No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTTACAGTCCAGTA460.10121902917748536No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)