FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002450223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002450223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32882
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA10563.2114834864059363No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA9102.767471564989964No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA6732.0467124870749953No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA4771.4506416884617723No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA3781.149565111611216No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA3731.1343592238914908No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA3441.0461650751170855No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA3431.0431238975731403No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNNAGTA3321.0096709445897452No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA3180.967094458974515No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCNAGTA3120.9488473937108449No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA2800.8515297123046044No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA2680.8150355817772641No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA2560.7785414512499239No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA2540.772459096162034No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA2160.6568943494921233No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA2140.6508119944042333No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNCAGCA1810.5504531354540477No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG1780.5413296028222128No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1570.4774648743993674No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA1510.4592178091356974No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA1470.4470530989599173No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGNTGGTGCCCAGTA1450.4409707438720272No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCCAGTT1440.4379295663280823No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNNAGCA1420.4318472112401922No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTNAGCGTCAGTTGGTGCNCAGTA1400.4257648561523022No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA1400.4257648561523022No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGNGTCAGTTGGTGCNNAGTA1390.42272367860835713No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCNAGTA1350.4105589684325771No Hit
CCTGTTTGCTACCNACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1340.40751779088863205No Hit
CNTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA1310.39839425825679703No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTNAGTTACAGTCCAGCA1280.38927072562496196No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACAGTTTAGCA1280.38927072562496196No Hit
CCTGTTTGCTACCCACACTTTNGAGCCTCAGCGTCAGTTGGTGCCCAGTA1240.37710601544918193No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGNGTCAGTTATCGTNNAGTA1220.37102366036129186No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA1220.37102366036129186No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1200.36494130527340185No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT1200.36494130527340185No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCNAGCA1160.35277659509762177No Hit
CCTGTTTGCTACCNACACTTTCGAGCNTCAGNGTNAGTTGGNGCNCAGTA1150.34973541755367676No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCNNAGTA1140.3466942400097318No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA1130.34365306246578675No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGAA1130.34365306246578675No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAGNGTNAGTTATNGTNCAGTA1110.33757070737789674No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCNCAGTA1100.3345295298339517No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGNTATCGTCCAGTA1100.3345295298339517No Hit
CCTGTTTGCNACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1090.33148835229000667No Hit
CCTGTTTGCTCCCCACGCTTTNGAGCCTCAGCGTCAGTTATCGTCCAGTA1080.32844717474606167No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA1060.32236481965817165No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA1030.3132412870263366No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCNAGTA1020.3102001094823916No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGNTGGTGCCCAGTA1020.3102001094823916No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA1010.30715893193844657No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTAAAGCCCAGTA990.30107657685055655No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGNGTCAGTTGGTGCNNAGTA990.30107657685055655No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNCAGTA970.2949942217626665No Hit
CCTGTTTGCNCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA970.2949942217626665No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTNAGCGTCAGTTATCGTNCAGTA950.2889118666747765No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNNAGTA920.27978833404294146No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG890.2706648014111064No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA870.2645824463232163No Hit
CCTGTTTGATACCNACACTTTCGAGCATCAGCGTCAGTTACAGTNCAGCA860.26154126877927136No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA840.2554589136913813No Hit
CNTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA840.2554589136913813No Hit
CCTGTTNGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA830.2524177361474363No Hit
CCTGTTTGCTACCCATGCTTTNGAGCCTCAGCGTCAGTTGGTGCCCAGTA770.2341706708837662No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA770.2341706708837662No Hit
CCTGTTTGCNACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA750.22808831579587616No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTNCAGTT750.22808831579587616No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTNNAGTT750.22808831579587616No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTNAGNTACAGTCCAGCA740.22504713825193112No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTNAGCGTCAGTTGGTGCNCAGTA720.21896478316404114No Hit
CCTGTTNGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA720.21896478316404114No Hit
CCTGTTTGCTACCNATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA700.2128824280761511No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTNCAGTG700.2128824280761511No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA690.20984125053220606No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA680.20680007298826106No Hit
CCTGTTTGCTACCNATGCTTTCGAGCNTCAGNGTNAGTTGGNGCNCAGTA680.20680007298826106No Hit
CCTGTTTGANACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNCAGCA670.20375889544431602No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTNCAGAA670.20375889544431602No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG660.20071771790037102No Hit
CCTGTTTGATACCNACACTTTCGAGCNTCAGNGTNAGTTACNGTNCAGCA640.19463536281248098No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGTTACCGTCCAGTA640.19463536281248098No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTNNAGTG620.18855300772459097No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCNAGTT610.18551183018064593No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTNNAGAA600.18247065263670093No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGNGTCAGTTACAGTNNAGCA600.18247065263670093No Hit
CCTGTTTGATACCCACACTTTNGAGCATCAGCGTCAGTTACAGTCCAGCA580.17638829754881089No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAANGTNAGTTACNGTNCAGTA580.17638829754881089No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA570.1733471200048659No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCCAGTA570.1733471200048659No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGNGTCAGTTAAAGCNNAGTA570.1733471200048659No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAACGTCAGTGGCGGCTTAGCA550.16726476491697584No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGNTAAAGCCCAGTA540.16422358737303083No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTNNGGTA540.16422358737303083No Hit
CNTGTTTGATACCCACACTTTCGAGCATCAGCGTNAGTTACAGTCCAGCA540.16422358737303083No Hit
CCTGTTNGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNCAGCA540.16422358737303083No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTNNAGAA530.16118240982908583No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCNAGTG520.15814123228514082No Hit
CCTGTTNGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA520.15814123228514082No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA510.1551000547411958No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA510.1551000547411958No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTAAAGCCCAGTA490.14901769965330577No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCNAGAA490.14901769965330577No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG490.14901769965330577No Hit
CCTGTTTGCTCCCCACGCTTTNGAGCCTCAGCGTCAGTTAAAGCCCAGTA460.13989416702147073No Hit
CCTGTTTGATACCCACACTTTCGAGCATNAGCGTCAGTTACAGTNCAGCA460.13989416702147073No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTNAGACACAGTCCAGAA450.1368529894775257No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCCTCAGCGTCAGTTAAAGCNCAGTA440.1338118119335807No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCNAGAA440.1338118119335807No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCNCAGTA430.13077063438963568No Hit
CCTGTTTGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAGAGCCCAGTA430.13077063438963568No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTNAGTTACAGTCCAGTG430.13077063438963568No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCNCAGTT420.12772945684569065No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA420.12772945684569065No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTGTCGTNCAGAA410.12468827930174563No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTNCAGAA410.12468827930174563No Hit
CCTGTTCGATACCCACGCTTTCGAGCCTCAGCGTCAGTCGCGAGTTGGTA400.12164710175780061No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGCCGTCCAGTT400.12164710175780061No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCNCAGAG400.12164710175780061No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCNAGTT400.12164710175780061No Hit
CCTGTTTGCNCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCNCAGTA390.11860592421385562No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNCAGTA390.11860592421385562No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA390.11860592421385562No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACAGTNTAGCA380.1155647466699106No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTNAGCGTCAGTTAAAGCNCAGTA370.11252356912596556No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCNNAGTT370.11252356912596556No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTNTGGTA370.11252356912596556No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAANGTCAGTTACCGTNNAGTA370.11252356912596556No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTNAGTTAAAGCCCAGTT360.10948239158202057No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA360.10948239158202057No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA360.10948239158202057No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTNGGTA350.10644121403807555No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA350.10644121403807555No Hit
CCTGTTTGCNCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNCAGTA340.10340003649413053No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCA340.10340003649413053No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTCAGTCCAGAA340.10340003649413053No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAGNGTNAGTTAANGCNCAGTA340.10340003649413053No Hit
CCTGTTTGCTCCCCACGCTTTNGAGCCTCAACGTCAGTTACCGTCCAGTA330.10035885895018551No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTNAGATATAGTTTGGTA330.10035885895018551No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCNAGCA330.10035885895018551No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTNCAGCA330.10035885895018551No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)