FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002450224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002450224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75236
Sequences flagged as poor quality0
Sequence length301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGA857211.393481843798181No Hit
TGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGG838111.139614014567496No Hit
TGGGGAATATTGGGCAATGGGCGCAAGCCTGACCCAGCAACGCCGCGTGA46686.204476580360466No Hit
TGAGGAATATTGGTCAATGGGCGAGAGCCTGAACCAGCCAAGTAGCGTGA33274.422085171992132No Hit
TGAGGAATATTGGTCAATGGACGAGAGTCTGAACCAGCCAAGTAGCGTGA31704.213408474666383No Hit
TGGGGGATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGG29153.87447498537934No Hit
TGGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGA27453.648519325854644No Hit
TGAGGAATATTGGTCAATGGACGCAAGTCTGAACCAGCCATGCCGCGTGC25753.4225636663299484No Hit
TGGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCAACGCCGCGTGA24323.232495082141528No Hit
TGGGGGATATTGCGCAATGGGGGCAACCCTGACGCAGCAACGCCGCGTGA22472.9866021585411238No Hit
TGAGGAATATTGGTCAATGGGCGAGAGCCTGAACCAGCCAAGTAGCGTGC21782.8948907437928653No Hit
TGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGA18762.4934871604019353No Hit
TCGGGAATATTGCGCAATGGAGGAAACTCTGACGCAGTGACGCCGCGTAT17802.365888670317401No Hit
TGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA16562.2010739539582116No Hit
TGGGGAATATTGGGCAATGGGGGAAACCCTGACCCAGCAACGCCGCGTGA16072.135945557977564No Hit
TGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCAACGCCGCGTGA15031.9977138603859852No Hit
TGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCGACGCCGCGTGC10911.4501036737731936No Hit
TGGGGAATATTGCACAATGGAGGAAACTCTGATGCAGCGACGCCGCGTGA10201.3557339571481737No Hit
TCGGGAATATTGCGCAATGGAGGAAACTCTGACGCAGTGACGCCGCGTGC8881.1802860332819396No Hit
TGGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCGACGCCGCGTGA8641.148386410760806No Hit
TGAGGAATATTGGTCAATGGGCGATGGCCTGAACCAGCCAAGTAGCGTGA8581.1404115051305226No Hit
TGGGGGATATTGGACAATGGGGGCAACCCTGATCCAGCGACGCCGCGTGA8581.1404115051305226No Hit
TGGGGAATATTGGGCAATGGAGGAAACTCTGACCCAGCAACGCCGCGTGG8261.0978786751023446No Hit
TGGGGAATCTTCCGCAATGGGCGAAAGCCTGACGGAGCAACGCCGCGTGA7040.935722260619916No Hit
TGGGGAATATTGCACAATGGAGGAAACTCTGATGCAGCGATGCCGCGTGA5960.7921739592748154No Hit
TAGGGAATATTGGGCAATGGGCGCAAGCCTGACCCAGCAACGCCGCGTGA5790.7695783933223457No Hit
TGGGGAATCTTGCGCAATGGGGGGAACCCTGACGCAGCGACGCCGCGTGC4770.6340049976075284No Hit
TGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCGATGCCGCGTGG4360.5795098091339252No Hit
TGGGGAATATTGGGCAATGGAGGCAACTCTGACCCAGCAATGCCGCGTGA4180.5555850922430751No Hit
TGAGGAATATTGGTCAATGGTCGGCAGACTGAACCAGCCAAGTCGCGTGA4030.5356478281673667No Hit
TGAGGAATATTGGTCAATGGCCGAGAGGCTGAACCAGCCAAGTCGCGTGA4010.5329895262906056No Hit
TAGGGAATCTTCGGCAATGGGGGCAACCCTGACCGAGCAACGCCGCGTGA3760.4997607528310915No Hit
TAGGGAATTTTCGGCAATGGACGGAAGTCTGACCGAGCAACGCCGCGTGA3130.4160242437131161No Hit
TGGGGGATATTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA3050.40539103620607153No Hit
TAGGGAATTTTCGGCAATGGGGGAAACCCTGACCGAGCAACGCCGCGTGA2910.3867829230687437No Hit
TCGAGAATCATTCACAATGGGGGCAACCCTGATGGTGCGACGCCGCGTGG2870.38146631931522146No Hit
TGGGGGATATTGCACAATGGGGGAAACCCTGATGCAGCAACGCCGCGTGA2860.38013716837684086No Hit
TGGGGAATATTGGGCAATGGGCGAAAGCCTTACCCAGCAACGCCGCGTGA2680.35621245148599073No Hit
TGGGGGATATTGCACAATGGAGGAAACTCTGATGCAGCGACGCCGCGTGA2610.34690839491732683No Hit
TGGGGAATATTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA2490.33095858365676006No Hit
TGAGGAATATTGGTCAATGGACGCGAGTCTGAACCAGCCAAGTAGCGTGA2470.3283002817799989No Hit
TGGGGAATTTTGGACAATGGGGGCAACCCTGATCCAGCCATGCCGCGTGC2390.31766707427295443No Hit
TGAGGAATATTGGTCAATGGGCGCAGGCCTGAACCAGCCAAGTAGCGTGA2260.3003881120740071No Hit
TGGGGAATATTGGGCAATGGGGGGAACCCTGACCCAGCAACGCCGCGTGG2160.2870966026902015No Hit
TGGGGAATATTGGGCAATGGGGGGAACCCTGACCCAGCAACGCCGCGTGA1950.2591844329842097No Hit
TGGGGAATATTGCACAATGGGGGGAACCCTGATGCAGCGACGCCGCGTGG1940.25785528204582914No Hit
TGGGGAATATTGCGCAATGGGGGCAACCCTGACGCAGCAACGCCGCGTGA1940.25785528204582914No Hit
TTAGGAATATTCGTCAATGGGGGAAACCCTGAACGAGCAATGCCGCGTGA1800.23924716890850123No Hit
TGGGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGA1750.2326014142165984No Hit
TGGGGAATATTGCGCAATGGGGGCAACCCTGACGCAGCAACGCCGCGTGC1630.21665160295603167No Hit
TAGGGAATTTTCGGCAATGGGGGAAACCCTGACCGAGCAATGCCGCGTGA1580.21000584826412885No Hit
TGGGGGATATTGCACAATGGAGGAAACTCTGATGCAGCAACGCCGCGTGA1580.21000584826412885No Hit
TGGGGGATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGA1580.21000584826412885No Hit
TGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGATGCCGCGTGG1460.19405603700356214No Hit
TGAGGAATATTGGTCAATGGACGAGAGTCTGAACCAGCCAAGTCGCGTGA1420.18873943325003986No Hit
TGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCGACGCCGCGTGA1350.17943537668137594No Hit
TGGGGAATATTGCACAATGGGGGAAACCTTGATGCAGCGACGCCGCGTGG1340.17810622574299537No Hit
TGAGGGATATTGGGCAATGGGGGAAACCCTGACCCAGCAACGCCGCGTGA1340.17810622574299537No Hit
TGGGGAATATTGGGCAATGGGGGAAACCCTGACCCAGCGACGCCGCGTGA1310.1741187729278537No Hit
TGGGGAATATTGCGCAATGGGGGCAACCCTGACGCAGCCATGCCGCGTGA1180.15683981072890638No Hit
TGGGGGATATTGCACAATGGAGGGAACTCTGATGCAGCGACGCCGCGTGA1140.15152320697538413No Hit
TGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGATGCCGCGTGA1140.15152320697538413No Hit
TGAGGAATATTGGTCAATGGGCGTAAGCCTGAACCAGCCAAGTCGCGTGA1130.15019405603700356No Hit
TCGGGAATATTGCGCAATGGAGGACACTCTGACGCAGTGACGCCGCGTAT1090.1448774522834813No Hit
TGGGGAATATTGGGCAATGGGGGCAACCCTGACCCAGCAACGCCGCGTGA1080.14354830134510074No Hit
TGAGGAATATTGGTCAATGGGCGAGAGCCTGAACCAGCCAAGTCGCGTGA1070.14221915040672017No Hit
TTAGGAATATTCGTCAATGGAGGAAACTCTGAACGAGCAATGCCGCGTGA1000.13291509383805625No Hit
TGGGGAATATTGGGCAATGGACGCAAGTCTGACCCAGCAACGCCGCGTGA960.127598490084534No Hit
TGGGGAATATTGGGCAATGGAGGGAACTCTGACCCAGCAATGCCGCGTGA900.11962358445425061No Hit
TGGGGAATATTGGGCAATGGGCGAAAGCCTTACCCAGCAATGCCGCGTGA860.11430698070072837No Hit
TGGGGGATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGA830.1103195278855867No Hit
TGGGGGATATTGCGCAATGGGGGGAACCCTGACGCAGCAACGCCGCGTGA760.10101547131692275No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)