FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002450247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002450247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73773
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA20152.731351578490776No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA12801.7350521193390538No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCTAGTG10211.383975167066542No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA8311.1264283681021512No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA7531.0206986295799276No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA7350.9962994591517221No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA7270.985455383405853No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA6670.904124815311835No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG6240.8458379081777887No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA5630.7631518306155369No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA5070.6872433003944532No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCNAGCA4940.669621677307416No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNNAGCA4870.6601331110297806No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA4810.6520000542203788No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNCAGCA4620.6262453743239397No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTNNAGTG4620.6262453743239397No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCNAGTG4620.6262453743239397No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA4520.6126902796416033No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTNTAGTG3790.5137380884605479No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA3520.4771393328182398No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGCA3360.4554511813265016No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCNAGTA3320.45002914345356704No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG3270.44325159611239884No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNCAGTA2960.40123080259715616No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCNNAGCA2910.39445325525598796No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA2860.38767570791481976No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGNGTCAGTTGGTGCNNAGTA2840.3849646889783525No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTNAGTTACAGTCCAGCA2820.3822536700418852No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNNAGTA2810.3808981605736516No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG2790.3781871416371843No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCNCAGCA2790.3781871416371843No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG2750.37276510376424976No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA2730.3700540848277825No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGNTGGTGCCCAGTA2610.35378797120897887No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTNNAGGG2600.3524324617407453No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCNAGCA2600.3524324617407453No Hit
CCTGTTTGCTACCNACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA2580.34972144280427797No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTAGTGCCCAGTA2520.3415883859948762No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTNCAGGG2510.3402328765266425No Hit
CCTGTTTGCTACCNACACTTTCGAGCNTCAGNGTNAGTTGGNGCNCAGTA2500.3388773670584089No Hit
CCTGTTTGCTACCCACACTTTNGAGCCTCAGCGTCAGTTGGTGCCCAGTA2490.3375218575901753No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCNAGGG2470.334810838653708No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA2430.3293888007807734No Hit
CNTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA2370.32125574397137163No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA2350.3185447250349044No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTNAGCGTCAGTTGGTGCNCAGTA2330.3158337060984371No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA2250.304989630352568No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTNAGCA2240.3036341208843344No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNNAGCA2220.3009231019478671No Hit
CCTGTTTGCNACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA2200.29821208301139984No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTNNAGTA2160.2927900451384653No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTNAGTTGCAGTCTAGTG2150.29143453567023164No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCNAGTA1970.2670353652420262No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCNAGTA1950.26432434630555895No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTNCAGTA1920.26025781790085806No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA1850.2507692516232226No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA1800.2439917042820544No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCACCGTCCAGTA1740.23585864747265262No Hit
CCTGTTNGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA1720.23314762853618534No Hit
CCTGTTTGATACCCACACTTTCGAGCATNAGCGTCAGTTACAGTNCAGCA1710.2317921190679517No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTNAGTCTTTGTCCAGGG1710.2317921190679517No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNNAGTA1710.2317921190679517No Hit
CCTGTTTGCTCCCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA1680.2277255906632508No Hit
CCTGTTTGATACCCACACTTTNGAGCATCAGCGTCAGTTACAGTCCAGCA1650.2236590622585499No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGNGTCAGTTACAGTNNAGCA1650.2236590622585499No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTNAGTTAAAGCCCAGCA1590.21552600544914807No Hit
CCTGTTTGATACCNACACTTTCGAGCNTCAGNGTNAGTTACNGTNCAGCA1570.2128149865126808No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGTGTCAGTAACAGTCCAGTG1550.21010396757621352No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTNTGGTA1550.21010396757621352No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAANGTNAGTTACNGTNCAGTA1540.2087484581079799No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGTTACCGTCCAGTA1520.20603743917151263No Hit
CCTGTTNGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNCAGCA1480.20061540129857808No Hit
CCTGTTTGATACCNACACTTTCGAGCATCAGCGTCAGTTACAGTNCAGCA1440.19519336342564353No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGTTGCAGCTTAGCA1390.18841581608447536No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTNNAGTG1380.1870603066162417No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCNTGGCA1370.18570479714800808No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA1370.18570479714800808No Hit
CNTGTTTGATACCCACACTTTCGAGCATCAGCGTNAGTTACAGTCCAGCA1370.18570479714800808No Hit
CCTGTTTGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA1370.18570479714800808No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGNGTCAGTTGCAGTNNAGTG1360.18434928767977443No Hit
CCTGTTNGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTNTAGTG1340.18163826874330716No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCNNGGCA1310.17757174033860626No Hit
CCTGTTTGANACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNCAGCA1310.17757174033860626No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTNAGNTACAGTCCAGCA1300.17621623087037264No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA1290.17486072140213899No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTNNGGTA1270.1721497024656717No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCNAGTA1260.1707941929974381No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTNCAGTG1260.1707941929974381No Hit
CCTGTTTGATACCNACACTTTCGAGCNTCAGNGTNAGTTGCNGTNTAGTG1260.1707941929974381No Hit
CCTGTTTGATACCNACACTTTCGAGCATCAGTGTCAGTTGCAGTNTAGTG1250.16943868352920444No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTTACCGTCCAGGT1230.1667276645927372No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA1200.16266113618803626No Hit
CCTGTTNGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCTAGTG1200.16266113618803626No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTNGGTA1190.16130562671980264No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTNNAGTA1190.16130562671980264No Hit
CCTGTTTGATACCCACACTTTNGAGCATCAGTGTCAGTTGCAGTCTAGTG1190.16130562671980264No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCNAGAG1180.159950117251569No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTNAGTTTCAGTCCAGTA1170.15859460778333537No Hit
CNTGTTTGATACCCACACTTTCGAGCATCAGTGTNAGTTGCAGTCTAGTG1170.15859460778333537No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGCA1170.15859460778333537No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTNAGNTGCAGTCTAGTG1120.1518170604421672No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCNAGTG1070.14503951310099902No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCNNAGAG1070.14503951310099902No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA1060.14368400363276537No Hit
CCTGTTTGANACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTNTAGTG1060.14368400363276537No Hit
CCTGTTTGATACCCACACTTTCGAGCATNAGTGTCAGTTGCAGTNTAGTG1030.13961747522806447No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTAGTGCNNAGTA1030.13961747522806447No Hit
CCTGTTTGCTCCCNACGCTTTCGCACNTGAGNGTNAGTCTTNGTNCAGGG1020.13826196575983082No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAANGTCAGTTACCGTNNAGTA1010.1369064562915972No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCCTCAACGTCAGTTACCGTNCAGTA1010.1369064562915972No Hit
CCTGTTTGCTCCCNACACTTTCGAGCCTCAGCGTCAGTTAAAGCNCAGCA1000.13555094682336358No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTNAGNTAAAGCCCAGCA970.13148441841866265No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTAGTGCNCAGTA950.12877339948219538No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTNNAGTA940.12741789001396175No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGNTACCGTCCAGTA940.12741789001396175No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTNGGCA940.12741789001396175No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCNCAGAG940.12741789001396175No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGNGTCAGTTAAAGCNNAGCA930.1260623805457281No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGNGTCAGTCTTTGTNNAGGG930.1260623805457281No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTATCGTCCAGTA920.12470687107749448No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA920.12470687107749448No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA910.12335136160926084No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTNTAGTA900.1219958521410272No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTNAGTA900.1219958521410272No Hit
CCTGTTTGCTCCCCACGCTTTNGAGCCTCAACGTCAGTTACCGTCCAGTA890.12064034267279357No Hit
CCTGTTTGCTCCCCACACTTTNGAGCCTCAGCGTCAGTTAAAGCCCAGCA880.11928483320455995No Hit
CCTGTTTGCTCCCNACGCTTTCGCACCTGAGCGTCAGTCTTTGTNCAGGG880.11928483320455995No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTNAACGTCAGTTACCGTNCAGTA880.11928483320455995No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTNCAGTG870.11792932373632631No Hit
CCTGTTNGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCNCAGCA870.11792932373632631No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTAGTGCCNAGTA860.11657381426809267No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTNAGNCTTTGTCCAGGG860.11657381426809267No Hit
CCTGTTTGCNCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNCAGTA860.11657381426809267No Hit
CCTGTTTGCNCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCNCAGCA850.11521830479985903No Hit
CNTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTNAGTTAAAGCCCAGCA840.1138627953316254No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTNAGATATAGTTTGGTA840.1138627953316254No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTCAGTCCAGAA830.11250728586339176No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTNAGCGTCAGTCTTTGTNCAGGG830.11250728586339176No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGTTACCGTCCAGTA830.11250728586339176No Hit
CCTGTTTGANACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA820.11115177639515812No Hit
CCTGTTTGATACCNACACTTTCGAGCNTCAANGTNAGTTGCNGCNTAGCA800.10844075745869086No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTNAGTCATGGCTTGGCA790.10708524799045722No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNCAGTA790.10708524799045722No Hit
CCTGTTTGCTCCCCACGCTTTNGCACCTGAGCGTCAGTCTTTGTCCAGGG790.10708524799045722No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCACCGTNNAGTA780.10572973852222359No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG780.10572973852222359No Hit
CCTGTTCGCTACCCACGCTCTCGTGCATCAGCGTCAGTTACAGTCTGGTA770.10437422905398995No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTNCAGTA770.10437422905398995No Hit
CCTGTTNGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA770.10437422905398995No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCACCGTNCAGTA760.10301871958575631No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCNAGTA760.10301871958575631No Hit
CCTGTTNGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA750.10166321011752268No Hit
CNTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGTTGCAGCTTAGCA750.10166321011752268No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTNAGTTACACTCCAGTG740.10030770064928904No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)