FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002450253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002450253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28594
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA12434.347065818003777No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA6242.182276001958453No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA5021.7556130656781144No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA4941.7276351682171085No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA4871.7031545079387285No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA4571.5982373924599564No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA3841.3429390781282786No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA3721.30097223193677No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA3081.0771490522487235No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCNAGTA2520.8813037700216828No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNNAGTA2430.8498286353780513No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCCAGTT2380.8323424494649228No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA2330.814856263551794No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA2220.7763866545429111No Hit
CCTGTTTGCTACCNATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA2060.7204308596208995No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA2050.7169336224382737No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCNNAGCA1850.6469888787857593No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA1790.6260054556900049No Hit
CCTGTTTGATACCCGCACCTTCGAGCCTCAACGTCAGTGGCGGGCTGGCA1780.6225082185073793No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCNCAGCA1680.5875358466811219No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGNGTCAGTTGGTGCNNAGTA1620.5665524235853676No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCNAGCA1610.5630551864027419No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGNTGGTGCCCAGTA1580.5525634748548647No Hit
CCTGTTTGCNACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1570.5490662376722389No Hit
CNTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA1560.5455690004896132No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA1520.5315800517591103No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTNNAGAA1480.5175911030286074No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1460.510596628663356No Hit
CCTGTTTGCTACCNATGCTTTCGAGCNTCAGNGTNAGTTGGNGCNCAGTA1430.5001049171154788No Hit
CCTGTTNGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1420.49660767993285304No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCNAGAA1390.48611596838497584No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTNCAGAA1360.47562425683709864No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA1360.47562425683709864No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA1350.47212701965447296No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA1330.46513254528922154No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTNAGCGTCAGTTGGTGCNCAGTA1320.4616353081065958No Hit
CCTGTTNGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1320.4616353081065958No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTACAGCTTAGCA1260.4406518850108414No Hit
CCTGTTTGCTACCCATGCTTTNGAGCCTCAGCGTCAGTTGGTGCCCAGTA1250.4371546478282157No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA1190.4161712247324614No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGTTGTCGTCCAGTA1110.38819332727145556No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTNAGTTAAAGCCCAGCA1030.36021542981044974No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT1010.35322095544519827No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNCAGTA1000.34972371826257254No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGTTTTCGTCCAGAA910.31824858361894104No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCNAGTT910.31824858361894104No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAGNGTNAGTTATNGTNCAGTA890.3112541092536896No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTNNAGTT880.30775687207106384No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTNCAGTT870.3042596348884381No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA860.3007623977058124No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCGGTA840.29376792334056095No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTCAGTCCAGAA840.29376792334056095No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGNGTCAGTTATCGTNNAGTA830.2902706861579352No Hit
CCTGTTTGCNCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA790.27628173742743234No Hit
CCTGTTTGCTCCCCACGCTTTNGAGCCTCAGCGTCAGTTATCGTCCAGTA780.2727845002448066No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCNAGTA760.26579002587955514No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA760.26579002587955514No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCNAGTA760.26579002587955514No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNNAGTA750.2622927886969294No Hit
CCTGTTTGATACCCGCACCTTCGAGCCTCAACGTCAGTGGCGGGNTGGCA750.2622927886969294No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA750.2622927886969294No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGNTATCGTCCAGTA740.2587955515143037No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTNAGCGTCAGTTATCGTNCAGTA730.255298314331678No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTNNAGTA710.24830383996642652No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGTTACCGTCCAGTA700.2448066027838008No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA690.24130936560117508No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA680.23781212841854932No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGNGTCAGTTAAAGCNNAGCA680.23781212841854932No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA680.23781212841854932No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCNNAGCA670.2343148912359236No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA670.2343148912359236No Hit
CCTGTTTGATACCCGCACCTTCGAGCCTCAACGTCAGTGGCGGGNNGGCA660.2308176540532979No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNCAGCA650.22732041687067217No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTTATGCCCCGGCA650.22732041687067217No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTNCAGTA640.22382317968804646No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG640.22382317968804646No Hit
CCTGTTTGCNCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCNCAGCA620.216828705322795No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCNAGCA610.2133314681401693No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTNAGCGTCAGTTTTCGTNCAGAA610.2133314681401693No Hit
CCTGTTTGCTCCCNACACTTTCGAGCCTCAGCGTCAGTTAAAGCNCAGCA590.2063369937749178No Hit
CNTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTNAGTTAAAGCCCAGCA580.2028397565922921No Hit
CCTGTTNGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCNCAGCA570.19934251940966638No Hit
CCTGTTTGCTACCNACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA560.19584528222704065No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA550.1923480450444149No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA550.1923480450444149No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTNAGNTAAAGCCCAGCA540.18885080786178918No Hit
CCTGTTTGCTCCCCACACTTTNGAGCCTCAGCGTCAGTTAAAGCCCAGCA530.18535357067916347No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGNGTCAGTTTTCGTNNAGAA510.178359096313912No Hit
CCTGTTTGATACCCGCACCTTCGAGCCTCAACGTCAGTGGCGGGCNGGCA510.178359096313912No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTNAGCGTCAGTTAAAGCNCAGCA500.17486185913128627No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCNAGCA500.17486185913128627No Hit
CCTGTTNGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA500.17486185913128627No Hit
CCTGTTTGCTCCCNACGCTTTCGCGCCTCAGCGTCAGTTTTCGTNCAGAA490.17136462194866056No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTNAGTAGCAGTCCAGTA490.17136462194866056No Hit
CNTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA490.17136462194866056No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA490.17136462194866056No Hit
CCTGTTTGCTACCCACACTTTNGAGCCTCAGCGTCAGTTGGTGCCCAGTA480.16786738476603483No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCNCAGCA480.16786738476603483No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTACAGCNTAGCA480.16786738476603483No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTACAGCTNAGCA470.16437014758340912No Hit
CCTGTTNGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA470.16437014758340912No Hit
CCTGTTTGCTCCCNACACTTTCGAGCNTCAGNGTNAGTTAANGCNCAGCA470.16437014758340912No Hit
CCTGTTTGCTCCCNACGCTTTCGCGCNTCAGNGTNAGTTTTNGTNCAGAA470.16437014758340912No Hit
CCTGTTTGCTCCCCACGCTTTNGCGCCTCAGCGTCAGTTTTCGTCCAGAA470.16437014758340912No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTNAGTTACAGTCCAGTT460.1608729104007834No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTNAGTTAAAGCCCAGCA450.15737567321815765No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTACAGCNNAGCA440.15387843603553192No Hit
CCTGTTTGCNCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTNCAGAA440.15387843603553192No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTNAGCGTCAGTTGGTGCNCAGTA430.1503811988529062No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGNTTTCGTCCAGAA430.1503811988529062No Hit
CCTGTTTGATACCCGCACCTTCGAGCCTCAACGTNAGTGGCGGGCTGGCA430.1503811988529062No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGNTGGTGCCCAGTA420.14688396167028048No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA420.14688396167028048No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA400.139889487305029No Hit
CCTGTTTGCNACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA400.139889487305029No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNNAGCA400.139889487305029No Hit
CCTGTTTGCTACCNACACTTTCGAGCNTCAGNGTNAGTTGGNGCNCAGTA400.139889487305029No Hit
CCTGTTNGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTNCAGAA400.139889487305029No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTNNAGCA390.1363922501224033No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG390.1363922501224033No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGTTGTCGTCNAGTA380.13289501293977757No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTNCAGCA370.12939777575715186No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTCAGTCNAGAA370.12939777575715186No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA360.12590053857452613No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCNAGTT360.12590053857452613No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTCAGTNCAGAA350.1224033013919004No Hit
CCTGTTTGATACCCGCACCTTCGAGCCTCAACGTCAGTGGCGGACTGGCA350.1224033013919004No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGNGTCAGTTGGTGCNNAGTA350.1224033013919004No Hit
CCTGTTTGCTCCCNACGCTTTCGTGCCTCAGTGTCAGTAGCAGTNCAGTA350.1224033013919004No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGNGTCAGTAGCAGTNNAGTA350.1224033013919004No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNNGGTA340.11890606420927466No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTCAGTNNAGAA340.11890606420927466No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCCTCAACGTCAGTTACCGTNCAGTA340.11890606420927466No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGTTGTCGTNCAGTA340.11890606420927466No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGCA330.11540882702664895No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA330.11540882702664895No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTNAGTTACAGCTTAGCA330.11540882702664895No Hit
CCTGTTTGCNCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNCAGTA320.11191158984402323No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCNAGCA320.11191158984402323No Hit
CCTGTTNGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA320.11191158984402323No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGAA320.11191158984402323No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTAACAGTCCAGTA320.11191158984402323No Hit
CNTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGTTTTCGTCCAGAA310.1084143526613975No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGTTGTCGTNNAGTA310.1084143526613975No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA310.1084143526613975No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTNNGGTA300.10491711547877178No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTNGGTA300.10491711547877178No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTTCTGTCCAGAA300.10491711547877178No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCNCAGTT300.10491711547877178No Hit
CCTGTTTGCNCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTNCAGTT300.10491711547877178No Hit
CNTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTNAGTAGCAGTCCAGTA300.10491711547877178No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTNAGTTACAGTCCAGCA290.10141987829614604No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)