FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002450257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002450257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43125
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA11672.706086956521739No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCA9282.1518840579710146No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA8722.022028985507246No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA6151.4260869565217391No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA4891.133913043478261No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA4691.087536231884058No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA4481.038840579710145No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA4200.9739130434782608No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCNNAGCA3690.8556521739130435No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA3630.8417391304347827No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNNAGTA3600.8347826086956521No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCNAGTA3470.8046376811594202No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCNAGCA3340.7744927536231884No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG3280.7605797101449275No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCNCAGCA3240.751304347826087No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA3150.7304347826086957No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA2970.688695652173913No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA2760.64No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCCAGTT2750.6376811594202898No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTCCAGGA2560.5936231884057971No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA2480.5750724637681159No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA2370.5495652173913044No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA2300.5333333333333333No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA2260.5240579710144928No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA2250.5217391304347827No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA2160.5008695652173913No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA2120.4915942028985507No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTNAGTTAGAGCCCAGCA2100.4869565217391304No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTGCTGTCCAGCA1840.4266666666666667No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAGCGTCAGTAAAAGCCCAGCA1670.3872463768115942No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTNNAGCA1610.37333333333333335No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA1580.3663768115942029No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA1570.3640579710144927No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCNAGCA1500.34782608695652173No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCNAGCA1490.34550724637681157No Hit
CCTGTTTGCTACCNATGCTTTCGAGCNTCAGNGTNAGTTGGNGCNCAGTA1490.34550724637681157No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGNTGGTGCCCAGTA1470.3408695652173913No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGNGTCAGTTGGTGCNNAGTA1460.33855072463768116No Hit
CCTGTTNGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1430.33159420289855074No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTNNAGAA1410.3269565217391304No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTNCAGCA1400.32463768115942027No Hit
CCTGTTTGCNACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1400.32463768115942027No Hit
CCTGTTTGCTACCCATGCTTTNGAGCCTCAGCGTCAGTTGGTGCCCAGTA1390.3223188405797101No Hit
CCTGTTTGCTACCNATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1390.3223188405797101No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTNAGCGTCAGTTGGTGCNCAGTA1350.3130434782608696No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCNNAGAG1340.31072463768115943No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGNGTCAGTTATCGTNNAGTA1300.3014492753623188No Hit
CCTGTTTGCTCCCNACACTTTCGAGCCTCAGCGTCAGTTAGAGCNCAGCA1290.2991304347826087No Hit
CCTGTTTGCTCCCCACACTTTNGAGCCTCAGCGTCAGTTAGAGCCCAGCA1270.2944927536231884No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA1250.2898550724637681No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNNAGCA1230.28521739130434787No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNCAGCA1230.28521739130434787No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAGNGTNAGTTATNGTNCAGTA1220.2828985507246377No Hit
CNTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA1220.2828985507246377No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCNAGAG1210.28057971014492755No Hit
CCTGTTTGCTCCCCACGCTTTNGAGCCTCAGCGTCAGTTATCGTCCAGTA1200.2782608695652174No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGNGTCAGTTAGAGCNNAGCA1200.2782608695652174No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCNAGAA1190.27594202898550724No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCNCAGAG1190.27594202898550724No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA1180.2736231884057971No Hit
CCTGTTTGCNCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCNCAGCA1160.2689855072463768No Hit
CCTGTTNGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1160.2689855072463768No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA1160.2689855072463768No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTNAGCGTCAGTTAGAGCNCAGCA1160.2689855072463768No Hit
CNTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTNAGTTAGAGCCCAGCA1140.26434782608695656No Hit
CCTGTTNGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCA1140.26434782608695656No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTNNAGGA1130.2620289855072464No Hit
CCTGTTTGCTCCCNACACTTTCGAGCNTCAGNGTNAGTTAGNGCNCAGCA1130.2620289855072464No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTNCAGAA1120.25971014492753625No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTNAGNTAGAGCCCAGCA1110.2573913043478261No Hit
CCTGTTNGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCNCAGCA1110.2573913043478261No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGNTATCGTCCAGTA1110.2573913043478261No Hit
CCTGTTTGCNCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA990.22956521739130434No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTNAGTTGTCGTCCAGCA990.22956521739130434No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTNCAGTT980.2272463768115942No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTCNAGGA960.2226086956521739No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCNAGTT940.2179710144927536No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGTTGTCGTCCAGCA940.2179710144927536No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA930.21565217391304345No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTNTGGTA920.21333333333333335No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCNAGCA910.2110144927536232No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTNCAGGA910.2110144927536232No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTNAGCGTCAGTTATCGTNCAGTA910.2110144927536232No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTNNGGTA900.20869565217391303No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA890.2063768115942029No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTNNAGTT880.20405797101449274No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGNTGGTGCCCAGTA860.19942028985507249No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAGCGTCAGTAAAAGCNNAGCA860.19942028985507249No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCNAGTA830.192463768115942No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCNCAGCA830.192463768115942No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA820.19014492753623188No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGTTTTCGTCCAGAA810.18782608695652175No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTNGGTA800.1855072463768116No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTNAGTGACGGCCCAGAG790.18318840579710144No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA780.1808695652173913No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGNGTCAGTTGGTGCNNAGTA780.1808695652173913No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNNAGTA770.17855072463768115No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTNAGCGTCAGTTGGTGCNCAGTA770.17855072463768115No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA730.16927536231884058No Hit
CCTGTTTGCTACCNACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA730.16927536231884058No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAGCGTCAGTAAAAGCNCAGCA720.16695652173913045No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCNNAGCA710.1646376811594203No Hit
CCTGTTTGCTACCCACACTTTNGAGCCTCAGCGTCAGTTGGTGCCCAGTA710.1646376811594203No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG690.16No Hit
CNTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA690.16No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTTTCACCCTAGCA670.15536231884057972No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA660.1530434782608696No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTGCTGTCNAGCA650.1507246376811594No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGTTACCGTCCAGTA640.14840579710144927No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNCAGTA630.14608695652173914No Hit
CCTGTTTGCTACCNACACTTTCGAGCNTCAGNGTNAGTTGGNGCNCAGTA620.14376811594202898No Hit
CCTGTTTGCNACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA610.14144927536231885No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTGCTGTNNAGCA610.14144927536231885No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNNAGCA600.1391304347826087No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTNAGATATAGTTTGGTA600.1391304347826087No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTNAGTTACAGTCCAGTT590.13681159420289854No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGCCGTCCAGTT580.1344927536231884No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA580.1344927536231884No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNCAGCA570.13217391304347828No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGNTGTCGTCCAGCA570.13217391304347828No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA570.13217391304347828No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGNGTCAGTGACGGCNNAGAG560.12985507246376812No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG560.12985507246376812No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGTTAGTA550.12753623188405797No Hit
CCTGTTTGCNCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTNCAGCA550.12753623188405797No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACNGTCAGTTGTCGTNNAGCA540.12521739130434784No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGNGTCAGTTGTCGTNNAGCA540.12521739130434784No Hit
CCTGTTNGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTNCAGCA530.12289855072463768No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTNAGTTAATGTCCAGGA520.12057971014492753No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAGCGTCAGTAAAAGCCNAGCA520.12057971014492753No Hit
CCTGTTTGCTCCCNACGCTTTCGCGCNTCACNGTNAGTTGTNGTNCAGCA520.12057971014492753No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCNAGCA510.11826086956521738No Hit
CCTGTTTGCNCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNCAGCA510.11826086956521738No Hit
CCTGTTTGCTCCCNACGCTTTCGCGCCTCACCGTCAGTTGTCGTNCAGCA500.11594202898550725No Hit
CNTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTNAGTTGTCGTCCAGCA500.11594202898550725No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTNAGTTAAAGCCCAGCA500.11594202898550725No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTNAGNTGTCGTCCAGCA500.11594202898550725No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTAGTGCCCAGTA490.1136231884057971No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTNACCGTCAGTTGTCGTNCAGCA480.11130434782608695No Hit
CNTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGTTTTCGTCCAGAA480.11130434782608695No Hit
CCTGTTTGCTCCCCACGCTTTNGCGCCTCACCGTCAGTTGTCGTCCAGCA480.11130434782608695No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTNAGTTGCTGTCCAGCA480.11130434782608695No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTNAGTTACAGTCCAGCA460.10666666666666667No Hit
CCTGTTNGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA460.10666666666666667No Hit
CCTGTTTGCTCCCNACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNCAGCA450.10434782608695652No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTT450.10434782608695652No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTGCTGTNCAGCA450.10434782608695652No Hit
CNTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTNAGTGACGGCCCAGAG440.10202898550724637No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAGCGTNAGTAAAAGCCCAGCA440.10202898550724637No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)