FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002450287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002450287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77874
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA21292.7339034851169837No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTCCGGTA21212.7236304800061637No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA13621.7489791201171123No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA8581.1017797981354496No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA8441.0838020391915144No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTNCGGTA8201.0529830238590543No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA7951.020879882887742No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA7831.0054703752215117No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTCNGGTA7350.9438323445565914No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA6930.8898990677247862No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTNNGGTA6900.8860466908082287No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTTATGCCCCGGCA5810.7460769961733056No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTNAGTTGCGCTCCGGTA5780.7422246192567481No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA5430.6972802218969104No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA5420.6959960962580579No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA5410.6947119706192053No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA5240.6728818347587128No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA5190.6664612065644502No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA4420.5675835323728073No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA4330.5560264016231349No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA3710.4764106120142795No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA3670.4712741094588695No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTNNAGCA3510.45072809923722934No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA3260.4186249582659167No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCNAGCA3110.3993630736831292No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTNAGNTGCGCTCCGGTA3110.3993630736831292No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTNCAGCA3060.39294244548886664No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGNTGGTGCCCAGTA2850.36597580707296407No Hit
CCTGTTTGCTACCNACACTTTCGAGCNTCAGNGTNAGTTGGNGCNCAGTA2780.3569869276009965No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA2690.34542979685132397No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGNGTCAGTTGGTGCNNAGTA2660.3415774199347664No Hit
CNTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTNAGTTGCGCTCCGGTA2650.34029329429591393No Hit
CCTGTTCGATACCNGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTNCGGTA2620.3364409173793564No Hit
CCTGTTTGCTACCCACACTTTNGAGCCTCAGCGTCAGTTGGTGCCCAGTA2610.3351567917405039No Hit
CCTGTTNGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTNCGGTA2570.33002028918509385No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA2560.32873616354624136No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTNAGCGTCAGTTGCGCTNCGGTA2530.32488378662968387No Hit
CNTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA2510.3223155353519788No Hit
CCTGTTCGANACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTNCGGTA2500.3210314097131263No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNNAGCA2500.3210314097131263No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCCAGTT2480.31846315843542133No Hit
CCTGTTTGCTACCNACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA2470.31717903279656884No Hit
CCTGTTTGCNACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA2450.3146107815188638No Hit
CCTGTTCGATACCCGCGCTTTNGAGCCTCAGCGTCAGTTGCGCTCCGGTA2430.3120425302411588No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTNAGCGTCAGTTGGTGCNCAGTA2400.30819015332460126No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGNGTCAGTTGCGCTNNGGTA2380.30562190204689627No Hit
CCTGTTCGATACCNGCGCTTTCGAGCNTCAGNGTNAGTTGCNCTNCGGTA2340.3004853994914862No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA2330.29920127385263373No Hit
CCTGTTNGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTCCGGTA2310.29663302257492874No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA2310.29663302257492874No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTT2210.28379176618640367No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTTATGCCNNGGCA2170.27865526363099363No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA2140.27480288671443615No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCNAGTA2070.26581400724246856No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTTATGCCNCGGCA2060.26452988160361607No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTTATGCCCNGGCA2040.2619616303259111No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGNGTCAGTTGGTGCNNAGTA2010.25810925340935353No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTNAGCA2010.25810925340935353No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGTTAGTA1970.25297275085394355No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCNNAGTA1960.251688625215091No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTAACAGTCTAGTG1950.2504044995762385No Hit
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGAATCCAGACCAGA1890.2426997457431235No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA1840.23627911754886097No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCNNAGCA1810.23242674063230348No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTNCAGAA1790.22985848935459843No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCNAGAA1780.22857436371574594No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCNCAGTA1780.22857436371574594No Hit
CCTGTTTGCTACCNATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1780.22857436371574594No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTNNAGAA1760.22600611243804095No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCCAGTA1760.22600611243804095No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTNAGTTGTCGTCCAGCA1720.2208696098826309No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCNCAGCA1700.21830135860492592No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGNTGGTGCCCAGTA1670.21444898168836837No Hit
CCTGTTNGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1650.21188073041066338No Hit
CCTGTTTGCTACCCATGCTTTNGAGCCTCAGCGTCAGTTGGTGCCCAGTA1650.21188073041066338No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTNAGCGTCAGTTGGTGCNCAGTA1630.20931247913295836No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA1610.20674422785525334No Hit
CNTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA1580.20289185093869586No Hit
CCTGTTTGCTACCNATGCTTTCGAGCNTCAGNGTNAGTTGGNGCNCAGTA1550.1990394740221383No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTCGCACCCTAGCA1530.19647122274443332No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGTTGCAGCTTAGCA1530.19647122274443332No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCNAGCA1520.1951870971055808No Hit
CCTGTTCGATACCCACGCCTTCGAGCTTCAGCGTCAGTGCGGCGCTGGCA1520.1951870971055808No Hit
CCTGTTNGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1490.1913347201890233No Hit
CCTGTTTGCNACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1440.18491409199476078No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTNTGGTA1380.17720933816164575No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTNAGTTATGCCCCGGCA1360.17464108688394073No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTNGGTA1340.1720728356062357No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTNNGGTA1310.1682204586896782No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA1270.16308395613426818No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCNAGCA1260.1617998304954157No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTAAAGCCCAGTA1250.16051570485656316No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTNAGTTAAAGCCCAGCA1220.15666332794000565No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNNAGCA1150.14767444846803812No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA1150.14767444846803812No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNCAGCA1130.1451061971903331No Hit
CCTGTTNGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA1070.13740144335721807No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTGCTGTCCAGCA1050.13483319207951305No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACNGTCAGTTGTCGTNNAGCA1040.13354906644066056No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNCAGTA1030.13226494080180803No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTNNAGTT1010.12969668952410304No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCNAGTA1010.12969668952410304No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGTTTTCGTCCAGAA1010.12969668952410304No Hit
CCTGTTTGCTCCCNACGCTTTCGCGCCTCACCGTCAGTTGTCGTNCAGCA1000.12841256388525052No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTNAGTTACAGTCCAGCA990.12712843824639802No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTNCAGTT980.1258443126075455No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCNAGTT980.1258443126075455No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA970.12456018696869302No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTNAGNTGTCGTCCAGCA970.12456018696869302No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTNACCGTCAGTTGTCGTNCAGCA970.12456018696869302No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNNAGTA940.1207078100521355No Hit
CCTGTTTGCTCCCCACGCTTTNGCGCCTCACCGTCAGTTGTCGTCCAGCA940.1207078100521355No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGNGTCAGTTATGCCNNGGCA930.11942368441328301No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNNAGTA920.11813955877443048No Hit
CCTGTTTGATACCNACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA920.11813955877443048No Hit
CCTGTTNGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTNCAGCA900.11557130749672549No Hit
CNTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTNAGTTGTCGTCCAGCA900.11557130749672549No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG890.11428718185787297No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTAAAAGCNNAGTT890.11428718185787297No Hit
CCTGTTNGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA880.11300305621902047No Hit
CCTGTTTGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTCCGGTA880.11300305621902047No Hit
CCTGTTTGCTCCCNACGCTTTCGCGCNTCACNGTNAGTTGTNGTNCAGCA880.11300305621902047No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGNTGCAGCTTAGCA850.10915067930246296No Hit
CCTGTTTGATACCNGCACTTTCGAGCNTCAGNGTNAGTTATNCCNCGGCA850.10915067930246296No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCNAGTA850.10915067930246296No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTA840.10786655366361043No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTNAGATATAGTTTGGTA840.10786655366361043No Hit
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGAATCCAGNCCAGA840.10786655366361043No Hit
CCTGTTTGANACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA840.10786655366361043No Hit
CCTGTTTGATACCNACACTTTCGAGCNTCAANGTNAGTTGCNGCNTAGCA840.10786655366361043No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG840.10786655366361043No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTNCAGAA830.10658242802475794No Hit
CCTGTTTGCNCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTNCAGCA820.10529830238590544No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA810.10401417674705292No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGNTAAAGCCCAGTA800.10273005110820042No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTNAGCGTCAGTTATGCCNCGGCA800.10273005110820042No Hit
CCTGTTTGATACCCACACTTTNGAGCCTCAATGTCAGTTGCAGCTTAGCA800.10273005110820042No Hit
CCTGTTNGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA800.10273005110820042No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTAACAGTNNAGTG790.10144592546934793No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAANGTCAGTTGCAGCNNAGCA790.10144592546934793No Hit
CCTGTTTGCTACCCACACTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA790.10144592546934793No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGCA780.10016179983049542No Hit
CNTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGTTGCAGCTTAGCA780.10016179983049542No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTAAAAGCNCAGTT780.10016179983049542No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)