FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002450354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002450354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56438
Sequences flagged as poor quality0
Sequence length301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGA596510.569120096388957No Hit
TGAGGAATATTGGTCAATGGGCGAGAGCCTGAACCAGCCAAGTAGCGTGA51889.19238810730359No Hit
TGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGG42757.574683723732237No Hit
TGAGGAATATTGGTCAATGGGCGCGAGCCTGAACCAGCCAAGTAGCGTGG42247.484319075799993No Hit
TGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCGACGCCGCGTGA32825.815230872816188No Hit
TGAGGAATATTGGTCAATGGACGAGAGTCTGAACCAGCCAAGTAGCGTGA27584.886778411708423No Hit
TGAGGAATATTGGTCAATGGGCGCGAGCCTGAACCAGCCAAGTAGCGTGA22243.940607392182572No Hit
TCGGGAATATTGCGCAATGGAGGAAACTCTGACGCAGTGACGCCGCGTGC21583.823664906623197No Hit
TGGGGGATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGG19883.5224494135157167No Hit
TGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCAACGCCGCGTGA14782.618802934193274No Hit
TGGGGAATATTGGGCAATGGGCGCAAGCCTGACCCAGCAACGCCGCGTGA13522.3955490981253766No Hit
TGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGA12572.227222793153549No Hit
TGGGGAATATTGGGCAATGGAGGAAACTCTGACCCAGCAACGCCGCGTGG11862.1014210283851305No Hit
TGGGGGATATTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA10901.9313228675714944No Hit
TGGGGAATATTGCACAATGGAGGAAACTCTGATGCAGCGACGCCGCGTGA10761.9065168857861723No Hit
TGGGGAATATTGGGCAATGGGGGAAACCCTGACCCAGCAACGCCGCGTGA10021.7753995534923277No Hit
TGGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCAACGCCGCGTGA9381.6620007796165706No Hit
TCGGGAATATTGCGCAATGGAGGAAACTCTGACGCAGTGACGCCGCGTAT9251.638966653673057No Hit
TGAGGAATATTGGTCAATGGCCGAGAGGCTGAACCAGCCAAGTCGCGTGA8281.4670966370176124No Hit
TGAGGAATATTGGTCAATGGATGAGAGTCTGAACCAGCCAAGTAGCGTGA7431.3164888904638719No Hit
TGAGGAATATTGGTCAATGGACGGAAGTCTGAACCAGCCATGCCGCGTGC6551.1605655763847054No Hit
TGAGGAATATTGGTCAATGGGCGAGAGCCTGAACCAGCCAAGTAGCGTGC5630.997554838938304No Hit
TTGGGAATATTGGACAATGGAGGAAACTCTGATCCAGTGACGCCGCGTGA4600.8150536872320069No Hit
TGGGGAATCTTCCGCAATGGGCGAAAGCCTGACGGAGCAACGCCGCGTGA4010.7105141925652929No Hit
TGAGGAATATTGGTCAATGGACGCAAGTCTGAACCAGCCATGCCGCGTGC3980.7051986250398667No Hit
TGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCGATGCCGCGTGG2910.5156100499663347No Hit
TGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCAACGCCGCGTGA2650.46954179807930824No Hit
TGGGGGATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGA2650.46954179807930824No Hit
TAGGGAATTTTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA2650.46954179807930824No Hit
TGGGGAATATTGGGCAATGGACGCAAGTCTGACCCAGCAACGCCGCGTGA2610.4624543747120734No Hit
TGGGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGA2470.4376483929267515No Hit
TGAGGAATATTGGTCAATGGTCGGCAGACTGAACCAGCCAAGTCGCGTGA2330.41284241114142955No Hit
TGGGGGATATTGGACAATGGGGGAAACCCTGATCCAGCGATGCCGCGTGA2240.39689570856515116No Hit
TGGGGAATATTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA1710.30298734894928947No Hit
TGGGGGATATTGCACAATGGGGGAAACCCTGATGCAGCAACGCCGCGTGA1580.27995322300577624No Hit
TGAGGGATATTGGTCAATGGGGGAAACCCTGAACCAGCAACGCCGCGTGA1520.269322087954924No Hit
TAGGGAATCTTCGGCAATGGGGGCAACCCTGACCGAGCAACGCCGCGTGA1510.26755023211311524No Hit
TGGGGAATATTGCACAATGGGGGGAACCCTGATGCAGCGACGCCGCGTGA1500.26577837627130657No Hit
TGGGGGATATTGCACAATGGAGGAAACTCTGATGCAGCGACGCCGCGTGA1490.26400652042949785No Hit
TGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA1460.25869095290407174No Hit
TGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCGACGCCGCGTGC1390.24628796201141076No Hit
TGAGGAATATTGGTCAATGGGCGATAGCCTGAACCAGCCAAGTCGCGTGA1380.244516106169602No Hit
TGGGGGATATTGCGCAATGGGGGCAACCCTGACGCAGCAACGCCGCGTGA1330.23565682696055848No Hit
TAGGGAATTTTCGGCAATGGGGGAAACCCTGACCGAGCAACGCCGCGTGA1240.2197101243842801No Hit
TTGGGAATATTGGACAATGGAGGGAACTCTGATCCAGTGACGCCGCGTGA1180.20907898933342786No Hit
TGGGGAATCTTGCGCAATGGGGGGAACCCTGACGCAGCGACGCCGCGTGC1170.2073071334916191No Hit
TGAGGAATATTGGGCAATGGAGGCAACTCTGACCCAGCCATGCCGCGTGA1160.2055352776498104No Hit
TCGGGAATATTGCGCAATGGAGGAAACTCTGACGCAGGGACGCCGCGTGC1150.20376342180800172No Hit
TGAGGAATATTGGTCAATGGGCGAGAGCCTGAACCAGCCAAGTCGCGTGA1040.18427300754810588No Hit
TTAGGAATATTCGTCAATGGGGGAAACCCTGAACGAGCAATGCCGCGTGA1030.18250115170629716No Hit
TGAGGAATATTGGTCAATGGGCGGAAGCCTGAACCAGCCATGCCGCGTGA980.17364187249725363No Hit
TGGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCGACGCCGCGTGA940.16655444913001877No Hit
TCGAGAATCATTCACAATGGGGGAAACCCTGATGGTGCGACGCCGCGTGG880.15592331407916651No Hit
TGGGGAATATTGCACAATGGGGGGAACCCTGATGCAGCGACGCCGCGTGG850.1506077465537404No Hit
TGGGGAATATTGCGCAATGGGGGCAACCCTGACGCAGCAACGCCGCGTGC810.14352032318650554No Hit
TCGGGAATATTGCGCAATGGAGGAAACTCTGACGCAGTGATGCCGCGTGC760.134661043977462No Hit
TCGGGAATATTGCGCAATGGAGGGAACTCTGACGCAGTGACGCCGCGTAT750.13288918813565329No Hit
TGGGGGATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGA720.12757362061022715No Hit
TGGGGAATATTGCGCAATGGGGGCAACCCTGACGCAGCCATGCCGCGTGA580.1027676388249052No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)