FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002450436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002450436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93326
Sequences flagged as poor quality0
Sequence length301
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGGAATATTGCGCAATGGAGGAAACTCTGACGCAGTGACGCCGCGTGC1173812.577416797034052No Hit
TGGGGGATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGG1055711.311960225446285No Hit
TGGGGAATATTGGGCAATGGGCGCAAGCCTGACCCAGCAACGCCGCGTGA72227.738465165120116No Hit
TCGGGAATATTGCGCAATGGAGGAAACTCTGACGCAGTGACGCCGCGTAT68337.321646700812207No Hit
TGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGA38784.155326489938496No Hit
TGAGGAATATTGGTCAATGGACGAGAGTCTGAACCAGCCAAGTAGCGTGA33863.628142211173735No Hit
TGGGGAATATTGGGCAATGGGGGGAACCCTGACCCAGCAACGCCGCGTGG32423.4738443734864886No Hit
TGAGGAATATTGGTCAATGGACGCAAGTCTGAACCAGCCATGCCGCGTGC31673.393480916357714No Hit
TGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGA29553.1663202108737116No Hit
TGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGG23702.539485245269271No Hit
TGGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCAACGCCGCGTGA22402.400188586246062No Hit
TGAGGAATATTGGTCAATGGGCGAGAGCCTGAACCAGCCAAGTAGCGTGA18031.9319375093757367No Hit
TGGGGAATATTGGGCAATGGGGGAAACCCTGACCCAGCAACGCCGCGTGA16541.7722821078799047No Hit
TGGGGAATATTGGGCAATGGAGGAAACTCTGACCCAGCAACGCCGCGTGG16111.7262070591260743No Hit
TGGGGAATATTGCACAATGGGCGCAAGCTTGATGCAGCAACGCCGCGTGA15881.7015622656065834No Hit
TGAGGAATATTGGTCAATGGACGCGAGTCTGAACCAGCCAAGTAGCGTGA14661.5708377086771104No Hit
TGGGGAATCTTCCGCAATGGGCGAAAGCCTGACGGAGCAACGCCGCGTGA14451.5483359406810535No Hit
TGAGGAATATTGGACAATGGATGGAAATCTGATCCAGCCATGCCGCGTGG11881.2729571609197865No Hit
TGAGGAATATTGGTCAATGGGCGGAAGCCTGAACCAGCCAAGTCGCGTGA8830.9461457685961039No Hit
TAGGGAATTTTCGTCAATGGGGGGAACCCTGAACGAGCAATGCCGCGTGA8400.9000707198422733No Hit
TAGGGAATATTGGGCAATGGGCGCAAGCCTGACCCAGCAACGCCGCGTGA8150.8732829007993486No Hit
TGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA8050.8625677731821786No Hit
TGGGGGATATTGCACAATGGAGGAAACTCTGATGCAGCGACGCCGCGTGA7740.8293508775689518No Hit
TGGGGGATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGA6750.7232711141589697No Hit
TGGGGAATATTGCACAATGGAGGAAACTCTGATGCAGCGACGCCGCGTGA6590.7061269099714977No Hit
TGGGGGATATTGCGCAATGGGGGCAACCCTGACGCAGCAACGCCGCGTGA6550.7018408589246298No Hit
TGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCGACGCCGCGTGA5210.5582581488545528No Hit
TGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCGACGCCGCGTGA4940.5293273042881941No Hit
TGAGGGATATTGGGCAATGGGGGAAACCCTGACCCAGCAACGCCGCGTGA4920.52718427876476No Hit
TAGGGAATCTTCGGCAATGGGGGCAACCCTGACCGAGCAACGCCGCGTGA4600.49289587038981636No Hit
TGGGGGATATTGCACAATGGGGGAAACCCTGATGCAGCAACGCCGCGTGA4050.4339626684953818No Hit
TGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCAACGCCGCGTGA3970.42539056640164585No Hit
TGGGGAATATTGGGCAATGGACGCAAGTCTGACCCAGCAACGCCGCGTGA3780.405031823929023No Hit
TGAGGGATATTGGTCAATGGGGGAAACCCTGAACCAGCAACGCCGCGTGA3670.3932451835501361No Hit
TGAGGAATATTGGTCAATGGCCGAGAGGCTGAACCAGCCAAGTCGCGTGA3630.38895913250326813No Hit
TGAGGAATATTGGTCAATGGGCGGAAGCCTGAACCAGCCAAGTAGCGTGC3310.3546707241283244No Hit
TCGGGAATTTTGCGCAATGGAGGAAACTCTGACGCAGTGACGCCGCGTAT3180.3407410582260035No Hit
TGGGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGA3160.3385980327025695No Hit
TGGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGA3160.3385980327025695No Hit
TGGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCGACGCCGCGTGA3050.32681139232368256No Hit
TGAGGAATATTGGTCAATGGGCGCGAGCCTGAACCAGCCAAGTAGCGTGC2980.31931080299166364No Hit
TGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCGACGCCGCGTGC2890.3096671881362107No Hit
TTAGGAATATTCGTCAATGGAGGAAACTCTGAACGAGCAATGCCGCGTGA2840.30430962432762576No Hit
TGGGGAATATTGGGCAATGGGCGAAAGCCTTACCCAGCAACGCCGCGTGA2360.2528770117652101No Hit
TGGGGAATATTGCACAATGGGGGGAACCCTGATGCAGCGACGCCGCGTGA2070.22180314167541734No Hit
TTAGGAATATTCGTCAATGGGGGAAACCCTGAACGAGCAATGCCGCGTGA1880.2014443992027945No Hit
TGGGGAATATTGCACAATGGGGGGAACCCTGATGCAGCGATGCCGCGTGG1850.19822986091764355No Hit
TGAGGAATATTGGTCAATGGGCGAGAGCCTGAACCAGCCAAGTCGCGTGA1850.19822986091764355No Hit
TGAGGAATATTGGTCAATGGACGGAAGTCTGAACCAGCCATGCCGCGTGC1810.19394380987077556No Hit
TAGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGA1790.19180078434734157No Hit
TGAGGAATATTGGTCAATGGACGAGAGTCTGAACCAGCCAAGTCGCGTGA1660.17787111844502068No Hit
TGGGGAATATTGGGCAATGGGGGGAACCCTGACCCAGCGACGCCGCGTGG1610.17251355463643572No Hit
TGGGGAATATTGGGCAATGGGGGCAACCCTGACCCAGCGACGCCGCGTGG1570.16822750358956776No Hit
TGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGT1560.16715599082785076No Hit
TGAGGAATATTGGTCAATGGGCGTAAGCCTGAACCAGCCAAGTCGCGTGA1540.16501296530441678No Hit
GCGCGAAACCTCCGCAATGTGAGAAATCGCGACGGGGGGATCCCAAGTGC1500.16072691425754881No Hit
TGAGGAATATTGGTCAATGGACGTAAGTCTGAACCAGCCAAGTCGCGTGA1240.132867582452907No Hit
TGGGGGATATTGCACAATGGAGGAAACTCTGATGCAGCAACGCCGCGTGA1190.12751001864432207No Hit
TGAGGAATATTGGTCAATGGTCGGCAGACTGAACCAGCCAAGTCGCGTGA1160.12429548035917108No Hit
TGAGGAATATTGGTCAATGGGCGCAGGCCTGAACCAGCCAAGTAGCGTGA1160.12429548035917108No Hit
TGGGGAATATTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA1120.12000942931230311No Hit
TGAGGAATATTGGTCAATGGATGAGAATCTGAACCAGCCAAGTAGCGTGC1120.12000942931230311No Hit
TGGGGAATCTTGCGCAATGGGGGGAACCCTGACGCAGCGACGCCGCGTGC1090.11679489102715214No Hit
TGGGGAATATTGGGCAATGGGGGGAACCCTGACCCAGCAACGCCGCGTGA1010.10822278893341619No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)