FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002450463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002450463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43294
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA690615.951402041853374No Hit
CCCGTTTGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA27566.365778167875456No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA17934.14145147133552No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA16733.8642768051000136No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTAACAGTCTAGTG14053.245253383840717No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGCA12762.947290617637548No Hit
CCTGTTTGCTCCCCACGCTTTCGTGACTCAGCGTCAGTAACAGTCCAGTA7971.8409017415808198No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG7661.769298286136647No Hit
CNCGTTTGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA6731.55448791980413No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA6081.4043516422598974No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGG5381.2426664202891855No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCATGTCCCAGAG4971.1479650759920543No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA4280.9885896429066383No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA4270.9862798540213426No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA3290.7599205432623458No Hit
CNTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTAACAGTCTAGTG3250.7506813877211623No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA3080.7114149766711322No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGAA2960.6836975100475816No Hit
CNTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGCA2750.6351919434563681No Hit
CCCGTTTGCTACCCTGGCTTTCGCAGCTCAGCGTCAGACACAGTCCAGAA2520.5820667990945627No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTGAACGTCAGTTACTGTCCAGCA2210.5104633436503904No Hit
CNTGTTTGCTCCCCACGCTTTCGTGACTCAGCGTCAGTAACAGTCCAGTA2140.49429482145331916No Hit
NNTGTTTGCTCCCNACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA2070.478126299256248No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAG2020.4665773548297686No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGCACGTCAGTTACTGTCCAGCA1980.45733819928858505No Hit
NNTGTTTGNTCNCCACGCTTTCGAGCCTGNACGTCAGTTACTGTCCAGCA1880.4342403104356262No Hit
CNTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG1780.41114242158266734No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAG1780.41114242158266734No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG1670.38573474384441264No Hit
CNTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA1510.3487781216796785No Hit
CNTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGG1490.3441585439090867No Hit
CNTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCATGTCCCAGAG1370.3164410772855361No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG1280.29565297731787316No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCTAGCA1260.29103339954728136No Hit
CCCGTTTGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGCA1200.2771746662355061No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG1050.2425278329560678No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTATCGTCCAGTA960.22173973298840488No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGTA960.22173973298840488No Hit
NNCGTTTGCTACCNTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA940.2171201552178131No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA890.20557121079133367No Hit
CNCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA890.20557121079133367No Hit
NNCGTTTGNTANCCTGGCTTTCGCATCTCNGCGTCAGACACAGTCCAGAA880.20326142190603777No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAGCGTCAGTTACTGTCCAGCA870.2009516330207419No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA860.198641844135446No Hit
CCCGTTTGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGTA830.19171247747955836No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA780.18016353305307897No Hit
CNTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA770.17785374416778305No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTTCAGCA710.16399501085600776No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGAA670.15475585531482422No Hit
CNCGTTTGCTACCCTGGCTTTCGCAGCTCAGCGTCAGACACAGTCCAGAA660.15244606642952832No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCT650.15013627754423245No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTGCACGTCAGTTACTGTCCAGCA600.13858733311775304No Hit
NNTGTTTGCTCCCNACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA600.13858733311775304No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCTGCA540.12472859980597772No Hit
CNTGTTTGCTCCCCACGCTTTCGCGCCTGAACGTCAGTTACTGTCCAGCA530.12241881092068185No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACCGTCCAGCA480.11086986649420244No Hit
NNTGTTTGATACCNACACTTTCGAGCATCAGTGTCAGTAACAGTCTAGTG470.10856007760890656No Hit
CNTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAG450.10394049983831477No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGTCCAGCA450.10394049983831477No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCTAGCA440.10163071095301889No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)