FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002450471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002450471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68325
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG39625.798755945847055No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA37425.476765459202341No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA22163.2433223563849247No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA19852.9052323454079767No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA18892.764727405781193No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA14602.1368459568240032No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTATTGTCCAGAA12871.8836443468715696No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG12301.8002195389681668No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA11391.6670325649469446No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA11041.6158068057080133No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAATTGACCCCTCGCG7941.1620929381631906No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA7031.0289059641419687No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA6140.8986461763629711No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTACAGCTTAGCA6090.8913282107574095No Hit
CCCGTTTGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA6070.8884010245151848No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTTATGGCTTGGCA5980.8752286864251737No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA5820.8518111964873765No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCACCCCGGAT5690.8327844859129163No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA4830.7069154774972558No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTTCTGTCCAGAA4780.6995975118916942No Hit
CCTGTTTGCTCCCCATGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT4320.6322722283205269No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT4320.6322722283205269No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA4230.6190998902305159No Hit
CCTGTTCTATCCCCACGCTTTCGTGCCTCAGCGTCAGTTACGGATTCGCC4190.6132455177460666No Hit
CCTATTTGCTCCCCACGCTTTCGGGAATGAGCGTCAGTTGCGGCCCAGAC4180.6117819246249543No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGCTTAGTG4040.5912916209293817No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG3890.5693377241126967No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA3750.5488474204171241No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA3740.5473838272960118No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTCAGTCCAGAA3630.531284302963776No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTT3480.509330406147091No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAATAGACCCCTCGCG3300.4829857299670692No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTGCACTCCGGAC3270.4785949506037322No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGAA3210.4698133918770582No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCGCTCCAGTG2930.42883278448591294No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTTATGCCCCGGCA2830.4141968532747896No Hit
CCTATTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTCTCTGTCTAGGT2810.4112696670325649No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG2770.4054152945481156No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA2720.39809732894255395No Hit
CCTGTTCGATACCCGCACCTTCGAGCATCAGCGTCAGTAATGCTGCGGCA2700.3951701427003293No Hit
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGAATCCAGACCAGA2670.3907793633369923No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA2640.38638858397365533No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGG2630.384924990852543No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA2600.380534211489206No Hit
CCTGTTTGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAGAGCCCAGTA2490.36443468715697036No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA2390.3497987559458471No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCCAGTA2290.33516282473472375No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA2170.31759970728137576No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGTA2080.3044273691913648No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCA1930.28247347237467985No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA1710.2502744237102086No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA1660.24295645810464692No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTCGCACCCTAGCA1640.24002927186242226No Hit
CCTGTTCGATACCCGCGCTTTCGAGCTTCAGCGTCAGTTGCGCCCCAGTG1630.2385656787413099No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTGCTGCCCCGGCA1620.23710208562019758No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAACGTCAGTTACAGTCCAGTA1610.23563849249908525No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA1560.2283205268935236No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA1520.22246615440907427No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA1510.22100256128796195No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTGACAGTCTAGTG1510.22100256128796195No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAA1500.21953896816684962No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTA1380.20197585071350166No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAG1350.19758507135016465No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCCAGTT1310.1917306988657153No Hit
CCTGTTCGCTCCCCCAGCTTTCGCTCCTCAGCGTCAGTGCGCGCCCAGAG1290.18880351262349068No Hit
CCTGTTCGCTACCCACGCTCTCGTGCATCAGCGTCAGTTACAGTCTGGTA1250.18294914013904134No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGCA1240.18148554701792902No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCA1220.17855836077570436No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGCGTCAATACCGGTCCAGAC1190.17416758141236738No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTT1120.16392242956458106No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA1120.16392242956458106No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG1110.16245883644346873No Hit
CCTGTTTGCTCCCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA1030.15075009147457008No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1030.15075009147457008No Hit
CCTGTTCGCTCCCCACGCTTTCGAGTCTCAGCGTCAGTTATAGTCCAGCA1010.14782290523234543No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA970.1419685327478961No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTGCTGTCCAGCA970.1419685327478961No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG920.13465056714233442No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCCTAGCA900.13172338090010977No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGTTAGTA890.13025978777899744No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTTTGCCCCAGGG890.13025978777899744No Hit
CCTGTTCGATACCCGCACTTTCGAGCGTCAGCGTCAGTTGCGCTCCAGTG860.12586900841566045No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTGATCGTCCAGTA860.12586900841566045No Hit
CCCATTTGCTACCCTGGCTTTCGTTCCTCAGTGTCAATTACGGCCCAGTA840.12294182217343577No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA830.12147822905232346No Hit
CCTGTTTGCTACCCACACTTTCGCGCCTCAGCGTCAGTTGGTGCCCAGTA800.11708744968898646No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTAATTGTCCAGAA800.11708744968898646No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG780.1141602634467618No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAGTGTCAGTAACAGTCCAGAA780.1141602634467618No Hit
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGCAACAGACCAGAC780.1141602634467618No Hit
CCTGTTCGATACCCGCACTTTCGCGCTTCAGCGTCAGTTGCGCTCCAGTG760.11123307720453714No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA760.11123307720453714No Hit
CCTGTTCGCTACCCACGCTCTCGCGCATCAGCGTCAGACCGAGCCTGGGA750.10976948408342481No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGTTGTCGTCCAGTA740.10830589096231247No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTGTCAGTCCAGAA730.10684229784120014No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGTTACAGTCCAGAA730.10684229784120014No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCTAGTG730.10684229784120014No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAGGCCCAGTA710.10391511159897548No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTGCGTCCCAGGG710.10391511159897548No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTGCAGTCCAGAA690.10098792535675083No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)