FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002450497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002450497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44687
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA10062.2512139995971983No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTTCAGTCCAGTA9322.0856177411775234No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA8901.9916306755879787No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA4931.1032291270391836No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTTCAGTNNAGTA3930.8794503994450288No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTTCAGTCNAGTA3790.848121377581847No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA3780.8458835903059057No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA3730.8346946539261978No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA3500.7832255465795421No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTTCAGTNCAGTA3200.7160919283012956No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNNAGTA3130.7004274173697048No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA3060.684762906438114No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA3040.6802873318862309No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCNAGTA3010.6735739700584062No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTCCGGTA2790.6243426499876922No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA2760.6176292881598675No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA2620.5863002662966859No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA2390.5348311589500302No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACGGTCCAGAA2370.5303555843981471No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA2370.5303555843981471No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA2120.4744109024996084No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA1980.4430818806364267No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTNAGTTTCAGTCCAGTA1930.4318929442567189No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1920.4296551569807774No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA1920.4296551569807774No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA1890.4229417951529528No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT1810.4050394969454204No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA1800.40280170966947887No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA1770.3960883478416542No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG1720.3848994114619464No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTT1610.3602837514265894No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA1510.3379058786671739No Hit
CCTGTTTGCTACCCACACTTTNGAGCCTCAGCGTCAGTTGGTGCCCAGTA1380.30881464407993375No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTNAGCGTCAGTTGGTGCNCAGTA1370.3065768568039922No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGNTGGTGCCCAGTA1360.3043390695280507No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1310.29315013314834293No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA1300.2909123458724014No Hit
CCTGTTTGCTACCNACACTTTCGAGCNTCAGNGTNAGTTGGNGCNCAGTA1290.2886745585964598No Hit
CCTGTTTGCTACCNACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1280.2864367713205183No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1240.2774856222167521No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA1220.27301004766486897No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAANGTCAGTTTCAGTNNAGTA1210.27077226038892743No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAGNGTNAGTTATNGTNCAGTA1200.2685344731129859No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTCATCGTCCAGTA1190.26629668583704436No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTT1180.26405889856110276No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTNCGGTA1160.2595833240092197No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA1160.2595833240092197No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA1150.25734553673327815No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGNGTCAGTTGGTGCNNAGTA1120.2506321749054535No Hit
CCTGTTTGCNACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1110.24839438762951196No Hit
CCTGTTTGCTCCCNACACTTTCGTGCCTCAACGTCAGTTTCAGTNCAGTA1070.23944323852574576No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCNNAGCA1070.23944323852574576No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCNAGTA1060.2372054512498042No Hit
CCTGTTTGCNCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA1060.2372054512498042No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA1050.23496766397386265No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA1050.23496766397386265No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTNAGCGTCAGTTATCGTNCAGTA1030.23049208942197957No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGNGTCAGTTATCGTNNAGTA1020.228254302146038No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCNCAGCA1020.228254302146038No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTNCAGTA1010.22601651487009644No Hit
CCTGTTTGCTCCCCACGCTTTNGAGCCTCAGCGTCAGTTATCGTCCAGTA1010.22601651487009644No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGNTATCGTCCAGTA1010.22601651487009644No Hit
CCTGTTTGCTCCCCACACTTTNGTGCCTCAACGTCAGTTTCAGTCCAGTA1000.2237787275941549No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTNNAGTA990.22154094031821334No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTA980.21930315304227183No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG970.21706536576633026No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACGGTCNAGAA940.21035200393850562No Hit
CNTGTTTGCTCCCCACACTTTCGTGCCTCAACGTNAGTTTCAGTCCAGTA940.21035200393850562No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACGGTNNAGAA940.21035200393850562No Hit
CCTGTTNGCTCCCCACACTTTCGTGCCTCAACGTCAGTTTCAGTNCAGTA940.21035200393850562No Hit
CNTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA940.21035200393850562No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA930.20811421666256405No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTNCAGCA920.2058764293866225No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTNAACGTCAGTTTCAGTNCAGTA920.2058764293866225No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACGGTNCAGAA910.20363864211068095No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCCAGTT900.20140085483473943No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG890.19916306755879787No Hit
CCTGTTNGCTCCCCACACTTTCGTGCCTCAACGTCAGTTTCAGTCCAGTA890.19916306755879787No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTNAGNTTCAGTCCAGTA880.1969252802828563No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCNAGCA870.19468749300691476No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTNNAGAA850.19021191845503166No Hit
CCTGTTTGCTCCCNACACTTTCGTGCNTCAANGTNAGTTTCNGTNCAGTA840.18797413117909012No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTNNAGTA840.18797413117909012No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGCA820.18349855662720702No Hit
CCTGTTTGCNCCCCACACTTTCGTGCCTCAACGTCAGTTTCAGTNCAGTA820.18349855662720702No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTNAGTAGCAGTCCAGTA820.18349855662720702No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG800.1790229820753239No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAACGTCAGTGGCGGCTTAGCA790.17678519479938237No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTNNGGTA790.17678519479938237No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNNAGTA760.17007183297155773No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTNAGTTGCGCTCCGGTA760.17007183297155773No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTNCAGTG760.17007183297155773No Hit
CCTGTTTGCTACCNATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA750.16783404569561616No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCNAGTA740.16559625841967462No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTNCAGAA740.16559625841967462No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCNAGCA730.16335847114373306No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTCNGGTA730.16335847114373306No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTNNAGTT710.15888289659184998No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNCAGTA700.15664510931590844No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG690.15440732203996688No Hit
CCTGTTTGCNACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA690.15440732203996688No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTNCAGTT690.15440732203996688No Hit
CCTGTTNGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA680.15216953476402534No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCNAGTT680.15216953476402534No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCNAGCA670.14993174748808377No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCNNAGTT670.14993174748808377No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCNAGTA660.14769396021214223No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCNAGAA660.14769396021214223No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTNNAGCA640.14321838566025916No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGNGTCAGTTGGTGCNNAGTA620.13874281110837605No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTNAGCGTCAGTTGGTGCNCAGTA610.13650502383243449No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTNCAGTA610.13650502383243449No Hit
CCTGTTTGCTACCNATGCTTTCGAGCNTCAGNGTNAGTTGGNGCNCAGTA600.13426723655649295No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNNAGCA600.13426723655649295No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTNNGGTA590.13202944928055138No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA590.13202944928055138No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNCAGCA570.1275538747286683No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTNAGTTAAAGCCCAGCA560.12531608745272674No Hit
CNTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA540.12084051290084363No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGTTACAGTCCAGTA540.12084051290084363No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTNTGGTA530.1186027256249021No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGTTGTCGTCCAGTA520.11636493834896054No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCNAGTG520.11636493834896054No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTAAAAGCCNAGTT510.114127151073019No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGNTGGTGCCCAGTA510.114127151073019No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTNAGNTGCGCTCCGGTA510.114127151073019No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTNAGACACAGTCCAGAA510.114127151073019No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGTTACCGTCCAGTA510.114127151073019No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGTTGTCGTCCAGCA510.114127151073019No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTNAGTTGCGCTCCAGTG500.11188936379707745No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTNNAGTG500.11188936379707745No Hit
CCTGTTTGCTCCCCACACTTTNGAGCCTCAGCGTCAGTTAAAGCCCAGCA500.11188936379707745No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNNAGCA490.10965157652113591No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCNCAGTT490.10965157652113591No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAANGTNAGTTACNGTNCAGTA490.10965157652113591No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCA480.10741378924519435No Hit
CCTGTTNGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA480.10741378924519435No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA480.10741378924519435No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTNAGTTGTCGTCCAGCA480.10741378924519435No Hit
CCTGTTNGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTNCAGTA470.10517600196925281No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTNAGTTACGGTCCAGAA470.10517600196925281No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTNGGTA460.10293821469331126No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTGCTGTCCAGCA460.10293821469331126No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCNAGTT460.10293821469331126No Hit
CCTGTTCGATACCNGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTNCGGTA450.10070042741736972No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)