FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002450593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002450593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41009
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA14913.6357872662098565No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA14563.5504401472847427No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA12363.0139725426125974No Hit
CCTGTTTGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA7071.7240118022873028No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCNNAGCA6141.497232314857714No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCNAGCA5431.3240995878953399No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCNCAGCA5421.321661098783194No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA5201.2680143383159794No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA5131.2509449145309566No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA5121.2485064254188105No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA4771.1631593064936965No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA4601.1217049915872126No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA4421.0778121875685827No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTNAGTTAAAGCCCAGCA3470.8461557219147017No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA3380.8242093199053866No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA2830.6900924187373503No Hit
CCTGTTTGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCNNAGCA2670.6510765929430125No Hit
CCTGTTTGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCNCAGCA2560.6242532127094053No Hit
CCTGTTTGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCCNAGCA2300.5608524957936063No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA2140.5218366699992685No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGNTGGTGCCCAGTA2130.5193981808871224No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGNGTCAGTTAAAGCNNAGCA2080.5072057353263918No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA2000.4876978224292229No Hit
CCTGTTTGCTACCNACGCTTTCGTGCCTCAGCGTCAGTTAAAGCNCAGCA1930.47062839864420003No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA1920.46818990953205397No Hit
CCTGTTNGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCNCAGCA1920.46818990953205397No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTNAGNTAAAGCCCAGCA1900.46331293130776174No Hit
CNTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTNAGTTAAAGCCCAGCA1880.45843595308346946No Hit
CCTGTTTGCTACCCACGCTTTNGTGCCTCAGCGTCAGTTAAAGCCCAGCA1850.4511204857470311No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1840.448681996634885No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA1830.44624350752273895No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGNTGGTGCCCAGTA1810.4413665292984467No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGNGTCAGTTGGTGCNNAGTA1760.4291740837377161No Hit
CCTGTTTGCNACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCNCAGCA1750.42673559462557004No Hit
CNTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA1730.4218586164012778No Hit
CCTGTTTGCTACCNATGCTTTCGAGCNTCAGNGTNAGTTGGNGCNCAGTA1730.4218586164012778No Hit
CCTGTTTGCTACCNACGCTTTCGTGCNTCAGNGTNAGTTAANGCNCAGCA1720.41942012728913164No Hit
CCTGTTTGCTACCNACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1700.4145431490648394No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTNAGCGTCAGTTAAAGCNCAGCA1660.404789192616255No Hit
CNTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA1650.4023507035041089No Hit
CCTGTTTGCTACCNACACTTTCGAGCNTCAGNGTNAGTTGGNGCNCAGTA1620.3950352361676705No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGNGTCAGTTGGTGCNNAGTA1610.3925967470555244No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1590.38771976883123216No Hit
CCTGTTNGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA1590.38771976883123216No Hit
CCTGTTTGCTACCCACACTTTNGAGCCTCAGCGTCAGTTGGTGCCCAGTA1580.38528127971908604No Hit
CCTGTTNGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1540.3755273232705016No Hit
CCTGTTTGCNACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1520.37065034504620936No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA1490.363334877709771No Hit
CCTGTTTGCTACCCATGCTTTCGTGCCTCAGCGTNAGTTAAAGCCCAGCA1470.3584578994854788No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTNAGCGTCAGTTGGTGCNCAGTA1440.35114243214904045No Hit
CCTGTTNGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1400.341388475700456No Hit
CCTGTTTGCTACCCATGCTTTNGAGCCTCAGCGTCAGTTGGTGCCCAGTA1390.3389499865883099No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTNAGCGTCAGTTGGTGCNCAGTA1360.33163451925187154No Hit
CCTGTTTGCNACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1340.3267575410275793No Hit
CCTGTTTGCTACCNATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1300.31700358457899486No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAACGTCAGTGGCGGTTTAGCA1270.3096881172425565No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTACAGCTTAGCA1130.2755492696725109No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGCA1020.24872588943890364No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT1020.24872588943890364No Hit
CCTGTTTGCTACCCATGCTTTCGTGCCTCAGNGTCAGTTAAAGCNNAGCA990.24141042210246533No Hit
CCTGTTTGCTACCNATGCTTTCGTGCCTCAGCGTCAGTTAAAGCNCAGCA950.23165646565388087No Hit
CCTGTTTGCTACCCATGCTTTCGTGCCTCAGCGTNAGNTAAAGCCCAGCA940.22921797654173473No Hit
CCCGTTCGCTCCCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA930.22677948742958862No Hit
CCTGTTTGCTACCNATGCTTTCGTGCNTCAGNGTNAGTTAANGCNCAGCA860.20971006364456582No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTNCAGCA830.2023945963081275No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAACGTCAGTGGCGGGTTAGCA820.19995610719598134No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA810.19751761808383525No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNCAGTA800.19507912897168914No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCNAGCA790.19264063985954302No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTNNAGCA780.1902021507473969No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTACAGCCCAGCA770.1877636616352508No Hit
CCTGTTTGCTACCCATGCTTTNGTGCCTCAGCGTCAGTTAAAGCCCAGCA770.1877636616352508No Hit
CCTGTTNGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCNCAGCA750.18288668341095857No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA720.17557121607452023No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCNAGTA710.1731327269623741No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNNAGTA700.170694237850228No Hit
CCTGTTTGCTACCCATGCTTTCGTGCCTNAGCGTCAGTTAAAGCNCAGCA700.170694237850228No Hit
CNTGTTTGCTACCCATGCTTTCGTGCCTCAGCGTNAGTTAAAGCCCAGCA660.16094028140164354No Hit
CCTGTTTGCNACCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCNCAGCA650.15850179228949743No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCNAGTA640.15606330317735131No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTNNAGTA590.14387085761662075No Hit
CCTGTTNGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA580.14143236850447463No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTNCAGTA570.1389938793923285No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNNAGTA570.1389938793923285No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA570.1389938793923285No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA560.1365553902801824No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA560.1365553902801824No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA550.13411690116803626No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNNAGCA540.13167841205589018No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCNAGCA510.12436294471945182No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA510.12436294471945182No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNCAGCA510.12436294471945182No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAACGTCAGTGGCGGCTTAGCA510.12436294471945182No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCNCAGCA500.12192445560730572No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCNNAGCA500.12192445560730572No Hit
CCTGTTCGATACCCGCACCTTCGAGCATCAGCGTCAGTGGTGCTGCGGCA490.11948596649515959No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGTTACCGTCCAGTA490.11948596649515959No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCNAGTA470.11460898827086737No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTAACAGTCCAGTA470.11460898827086737No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTACAGCNTAGCA470.11460898827086737No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTACAGCNNAGCA460.11217049915872125No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG460.11217049915872125No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAACGTCAGTGGCGGTNNAGCA450.10973201004657514No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA440.10729352093442902No Hit
CCCGTTCGCTCCCCTGGCTTTCGCATCTCAGCGTCAGACACAGTNCAGAA440.10729352093442902No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAACGTCAGTGGCGGTTNAGCA430.10485503182228291No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCNAGTT430.10485503182228291No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAACGTCAGTGGCGGTNTAGCA420.1024165427101368No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA420.1024165427101368No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTNAGTTGTCGTCCAGCA420.1024165427101368No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)