FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002450636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002450636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115699
Sequences flagged as poor quality0
Sequence length301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGGAATATTGGTCAATGGACGAGAGTCTGAACCAGCCAAGTAGCGTGA2499021.599149517281912No Hit
TGAGGAATATTGGTCAATGGGCGCGAGCCTGAACCAGCCAAGTAGCGTGA1246510.773645407479753No Hit
TGAGGAATATTGGTCAATGGGCGATGGCCTGAACCAGCCAAGTAGCGTGA67905.868676479485561No Hit
TGAGGAATATTGGTCAATGGACGCAAGTCTGAACCAGCCATGCCGCGTGC41413.5791147719513563No Hit
TGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGT39893.447739392734596No Hit
TGAGGAATATTGGTCAATGGGCGAGAGCCTGAACCAGCCAAGTAGCGTGA39243.391559131885323No Hit
TGAGGAATATTGGTCAATGGCCGAGAGGCTGAACCAGCCAAGTCGCGTGA38503.3276000656876894No Hit
TGAGGAATATTGGTCAATGGGCGCTAGCCTGAACCAGCCAAGTAGCGTGA38313.3111781432855945No Hit
TGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGG36563.159923594845245No Hit
TGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA34382.9715036430738384No Hit
TGGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGA34242.95940327919861No Hit
TGAGGAATATTGGTCAATGGTCGGCAGACTGAACCAGCCAAGTCGCGTGA31702.73986810603376No Hit
TGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGA25362.191894484827008No Hit
TGGGGGATATTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA22961.984459675537386No Hit
TGAGGAATATTGGTCAATGGACGCGAGTCTGAACCAGCCAAGTAGCGTGA22541.9481585839117017No Hit
TGGGGAATATTGGGCAATGGGCGCAAGCCTGACCCAGCAACGCCGCGTGA22071.907535933759151No Hit
TGGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCAACGCCGCGTGA15511.3405474550341836No Hit
TGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGA13581.1737352958971123No Hit
TGAGGAATATTGGTCAATGGGCGCAGGCCTGAACCAGCCAAGTAGCGTGA10750.9291350832764328No Hit
TGAGGAATATTGGTCAATGGACGGAAGTCTGAACCAGCCATGCCGCGTGC9450.8167745615778874No Hit
TGGGGAATATTGGGCAATGGGGGAAACCCTGACCCAGCAACGCCGCGTGA8490.7338006378620385No Hit
TGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCAACGCCGCGTGA7670.6629270780214177No Hit
TGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCGACGCCGCGTGA7370.6369977268602149No Hit
TGAGGAATATTGGTCAATGGGCGTAAGCCTGAACCAGCCAAGTCGCGTGA7110.6145256225205058No Hit
TAGGGAATATTGGGCAATGGGCGCAAGCCTGACCCAGCAACGCCGCGTGA7030.6076111288775183No Hit
TGGGGAATTTTGGACAATGGGCGCAAGCCTGATCCAGCTATTCCGCGTGT6240.5393305041530178No Hit
TGGGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGA5970.5159940881079352No Hit
TCGAGAATCATTCACAATGGGGGAAACCCTGATGGTGCGACGCCGCGTGG5290.45722089214254236No Hit
TGAGGAATATTGGTCAATGGACGAGAGTCTGAACCAGCCAAGTCGCGTGA5040.4356130995082067No Hit
TGGGGGATATTGGACAATGGGGGCAACCCTGATCCAGCGACGCCGCGTGA4440.38375439718580107No Hit
TGAGGAATATTGGTCAATGGGCGAAAGCCTGAACCAGCCAAGTAGCGTGA4310.37251834501594655No Hit
TGAGGAATCTTGGTCAATGGACGAGAGTCTGAACCAGCCAAGTAGCGTGA4310.37251834501594655No Hit
TGGGGAATATTGGGCAATGGGCGAAAGCCTTACCCAGCAACGCCGCGTGA4150.35868935772997174No Hit
TGAGGAATATTGGTCAATGGGCGAGAGCCTGAACCAGCCAAGTCGCGTGA3970.34313174703325006No Hit
TGAGGAATATTGGTCAATGGACGTAAGTCTGAACCAGCCAAGTCGCGTGA3870.3344886299795158No Hit
TGGGGGATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGG3710.320659642693541No Hit
TGAGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCCATGCCGCGTGC3180.2748511223087494No Hit
TGAGGGATATTGGGCAATGGGGGAAACCCTGACCCAGCAACGCCGCGTGA3010.2601578233174012No Hit
TGAGGAATATTGGTCAATGGGCGGTAGCCTGAACCAGCCAAGTAGCGTGA2530.21867086145947673No Hit
TGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCGACGCCGCGTGC2080.17977683471767258No Hit
TGGGGAATATTGGGCAATGGACGCAAGTCTGACCCAGCAACGCCGCGTGA1740.1503902367349761No Hit
TGGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCGACGCCGCGTGA1680.14520436650273555No Hit
TGGGGGATATTGCACAATGGAGGAAACTCTGATGCAGCGACGCCGCGTGA1670.14434005479736212No Hit
TAGGGAATTTTCGTCAATGGGGGGAACCCTGAACGAGCAATGCCGCGTGA1620.14001849627049498No Hit
TGGGGGATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGA1540.13310400262750757No Hit
TGGGGAATATTGCACAATGGAGGAAACTCTGATGCAGCGACGCCGCGTGA1510.1305110675113873No Hit
TAGGGAATTTTCGGCAATGGGGGAAACCCTGACCGAGCAACGCCGCGTGA1430.12359657386839991No Hit
TGAGGAATATTGGTCAATGGGCGCGAGCCTGAACCAGCCAAGTCGCGTGA1400.12100363875227962No Hit
TGAGGAATATTGGTCAATGGACGAGAGCCTGAACCAGCCAAGTAGCGTGA1350.11668208022541249No Hit
TGGGGGATATTGCACAATGGAGGGAACTCTGATGCAGCAACGCCGCGTGA1330.11495345681466565No Hit
TGGGGAATATTGCACAATGGGGGGAACCCTGATGCAGCGATGCCGCGTGG1290.11149620999317193No Hit
TGGGGGATATTGCACAATGGGGGAAACCCTGATGCAGCAACGCCGCGTGA1160.10026015782331742No Hit
TGGGGGATATTGCACAATGGGGGAAACCCTGATGCAGCAACGCCGCGTGT1160.10026015782331742No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)