FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002450639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002450639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50104
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA46599.298658789717388No Hit
CCTGTTTGATACCCGCACCTTCGAGCCTCAGCGTCAGTAATGCCCCGGTA27245.43669168130289No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA25285.045505348874341No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTAACAGTCTAGTG15563.1055404758103142No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA15093.0117355899728566No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG13222.6385118952578637No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACAGTTTAGCA12672.5287402203416893No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA10011.9978444834743734No Hit
CCTGTTTGCTACCCACACTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA8471.690483793709085No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA7891.5747245728883923No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA6801.3571770716908829No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA6501.297301612645697No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA6001.1975091809037202No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA4780.9540156474532971No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA3880.7743892703177391No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG2900.5787961041034648No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA2670.5328915855021555No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCGCATCCCAGGG2400.4790036723614881No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGCCGTCCAGTT2370.4730161264569695No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA2290.4570493373782532No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA2150.42910745649049975No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCTAGTG2000.3991697269679068No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG1860.37122784608015325No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG1840.3672361488104742No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTTACCGTCCAGGT1780.355261057001437No Hit
CCTGTTTGCTACACACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1590.31733993293948587No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG1430.28540635478205334No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAG1370.2734312629730161No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA1170.23351429027622544No Hit
CCTGTTTGATACACACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA1070.2135558039278301No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA1010.20158071211879292No Hit
CCTGTTTGCTACCCACACTTTCGCGCCTCAGCGTCAGTTGGTGCCCAGTA1000.1995848634839534No Hit
CCTGTTTGATACACGCACCTTCGAGCCTCAGCGTCAGTAATGCCCCGGTA990.19758901484911384No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCCTAGCA870.17363883123103943No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTATCGTCCAGTA870.17363883123103943No Hit
CCTGTTTGATACACACACTTTCGAGCATCAGTGTCAGTAACAGTCTAGTG860.17164298259619992No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCA820.16365958805684178No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTAGTGCCCAGTA810.16166373942200224No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGCG800.1596678907871627No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG770.1536803448826441No Hit
CCTGTTTGCTACCCACACTTTCGAGACTCAGCGTCAGTTGGTGCCCAGTA710.1417052530736069No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA700.13970940443876737No Hit
CCTGTTTGATACCCGCACCTTCGAGCCTCAGCGTCAGTAATCCCCCGGTA680.1357177071690883No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTAGTGCCCAGTA660.13172600989940922No Hit
CCTGTTTGATACCCACACTTTCGCGCCTCAATGTCAGTTGCAGCTTAGCA640.12773431262973017No Hit
CCTGTTTGATACACACACTTTCGAGCATGAACGTCAGTTACAGTTTAGCA630.1257384639948906No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA620.1237426153600511No Hit
CCTGTTTGATACCCGCACCTTCGCGCCTCAGCGTCAGTAATGCCCCGGTA620.1237426153600511No Hit
CCTGTTCGATACCCACGCTTTCGTGCTTCAGCGTCAGTTATAGCCCAGTA610.12174676672521156No Hit
CCTGTTTGATACCCACACTTTCGAGACTCAATGTCAGTTGCAGCTTAGCA580.11575922082069295No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCCGCGTCAGTTGGTGCCCAGTA560.1117675235510139No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGCGCCCAGTA560.1117675235510139No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA540.10777582628133482No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCCGTTGGTGCCCAGTA540.10777582628133482No Hit
CCTGTTTGCTACCCACACTCTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA530.1057799776464953No Hit
CCTGTTTGATACACACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA510.10178828037681621No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)