FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002450685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002450685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128184
Sequences flagged as poor quality0
Sequence length301
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT1712813.362042064532234No Hit
CCTGTTTGCTCCCCATGCTTTCGTACCTCAGTGTCAGTATTAGGCCAGAT100787.862135679960057No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGTGTCAGTATTATGCCAGAA40633.1696623603569867No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG25842.015852212444611No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG13051.0180677775697433No Hit
CCTGTTTGCTCCCCACGCCTTCGCACCTCAGTGTCAGTATCAGTCCAGGT8010.6248829807152219No Hit
CCTGTTTGCTCCCCACGCTCTCGCACCTCAGTGTCAGTATCAGTCCAGGT7770.6061598951507209No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGCT7350.573394495412844No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATGGTCCAGAA7130.5562316669787181No Hit
CCTGTTCGCTCCCCACGCTTTCGTTCATCAGCGTCAATCAGTACGTAGTA6690.5219060101104662No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCAGTCCAGGT6610.5156649815889659No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCCGGT5330.41580852524496037No Hit
CCTGTTTGCTCCCCATGCTTTCGTACCTCAGTGTCAGTATTAGGCCAGCT5280.41190788241902265No Hit
CCTGTTTGCTCCCCATGCTCTCGTACCTCAGTGTCAGTATTAGGCCAGAT4980.3885040254633964No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGTGTCAGTATTATGCCAGCA4530.3533982400299569No Hit
CCTGTTTGCTCCCCATGCTTTCGTACCTCAGCGTCAGTATTAGGCCAGAT4190.32687386881358044No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATTAGTCCAGGT3590.2800661549023279No Hit
CCTGTTTGCTCCCCATGCCTTCGTACCTCAGTGTCAGTATTAGGCCAGAT3250.2535417836859514No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCTAGGT3050.23793921238220056No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCCGTATCAGTCCAGGT2950.23013792673032518No Hit
CCTGCTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT2710.2114148411658241No Hit
CCTGTTTGCTCCACACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT2450.191131498470948No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCCGTCCAGGT2400.1872308556450103No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGTGTCAATATGATGCCAGAA2390.18645072707982277No Hit
CCTGTTTGCTCCCCATGCTTTCGTACCTCAGTGTCCGTATTAGGCCAGAT2300.1794295699931349No Hit
CCTGTTTGCTACCCACGCCTTCGAGCCTCAGTGTCAGTATTATGCCAGAA2300.1794295699931349No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCCGTGTCAGTATCAGTCCAGGT2250.17552892716719715No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAATCTTGACCCAGGG2220.17318854147163454No Hit
CCTGTTCGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT2180.17006802721088435No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTATTATGCCAGAA2110.16460712725457155No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGACCAGAG2090.16304687012419647No Hit
CCTGTTTGCTACCCACGCTCTCGAGCCTCAGTGTCAGTATTATGCCAGAA2080.16226674155900891No Hit
CCTGTTTGCTCCCCACACTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT2080.16226674155900891No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGTGTCAGTATTATGCCAGAA2050.1599263558634463No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTCAGTGTCAGTATTAGGCCAGAT2040.15914622729825875No Hit
CCTGTTTGCTCCCCACGCTTTCGCAACTCAGTGTCAGTATCAGTCCAGGT1960.1529051987767584No Hit
CCTGTTTGCTCCCCATGCTTTCGTACCTCAGTGTCAGTATTCGGCCAGAT1900.14822442738563316No Hit
CCTGCTTGCTCCCCATGCTTTCGTACCTCAGTGTCAGTATTAGGCCAGAT1860.145103913124883No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCACAGTGTCAGTATCAGTCCAGGT1770.1380827560381951No Hit
CCTGTTTGCTCCCCACGCTTTCGCATCTGAGCGTCAGTCTTTGTCCAGGG1740.13574237034263248No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT1740.13574237034263248No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGCG1630.1271609561255695No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGTT1610.1256006989951944No Hit
CCTGTTTGCTCCCCATGCTTTCGTACCTCCGTGTCAGTATTAGGCCAGAT1600.12482057043000687No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCAGTCCAGCT1500.11701928477813144No Hit
CCTGTTTGCTCCCCACGCCTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG1460.11389877051738127No Hit
CCTGTTTGCTCCCCACGCTCTCGCACCTGAGCGTCAGTCTTTGTCCAGGG1420.11077825625663108No Hit
CCTGTTTGCTCCACATGCTTTCGTACCTCAGTGTCAGTATTAGGCCAGAT1370.10687761343069338No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTCTTTGTCCAGGG1350.10531735630031828No Hit
CCTGTTTGCTCCCCATGCTTTCGTACCACAGTGTCAGTATTAGGCCAGAT1340.10453722773513074No Hit
CCTGTTTACTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT1330.1037570991699432No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCTGGT1300.10141671347438058No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)