FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002450715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002450715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24112
Sequences flagged as poor quality0
Sequence length301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA6412.6584273390842736No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTTCAGTCCAGTA4942.048772395487724No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGAA4111.7045454545454544No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA3531.4640013271400132No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA3471.4391174518911745No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA2761.1446582614465826No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA2621.0865958858659588No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA2501.0368281353682813No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA2400.9953550099535502No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA2280.9455872594558725No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTTCAGTNNAGTA2050.8501990710019907No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTTCAGTCNAGTA1980.8211678832116789No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTTCAGTNCAGTA1880.7796947577969475No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA1710.7091904445919044No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTNNAGAA1570.6511280690112806No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA1510.626244193762442No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCNCAGCA1500.6220968812209687No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA1460.6055076310550763No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCNAGAA1460.6055076310550763No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACGGTCCAGAA1440.5972130059721301No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA1420.5889183808891838No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTNCAGAA1250.5184140676841407No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNNAGCA1190.4935301924353019No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNCAGCA1190.4935301924353019No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG1170.4852355673523556No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCNAGCA1160.4810882548108826No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCNNAGCA1150.4769409422694095No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCNAGCA1150.4769409422694095No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTNAGTTTCAGTCCAGTA1100.45620437956204374No Hit
CCTGTTNGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1040.43132050431320507No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT1040.43132050431320507No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA1020.4230258792302588No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGTTGTCGTCCAGCA1000.41473125414731254No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA970.4022893165228932No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA950.3939946914399469No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGNTGGTGCCCAGTA930.38570006635700066No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTNAGTTACTGTCCAGAA880.36496350364963503No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA880.36496350364963503No Hit
CCTGTTNGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA860.35666887856668883No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTNCAGCA850.35252156602521567No Hit
CNTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA850.35252156602521567No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGNGTCAGTTGGTGCNNAGTA850.35252156602521567No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTNAGCGTCAGTTGGTGCNCAGTA840.3483742534837425No Hit
CCTGTTTGCNACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA820.34007962840079625No Hit
CCTGTTTGCTACCCATGCTTTNGAGCCTCAGCGTCAGTTGGTGCCCAGTA820.34007962840079625No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA820.34007962840079625No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTNNAGCA800.33178500331785005No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA780.3234903782349038No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA770.3193430656934307No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACGGTCNAGAA760.3151957531519575No Hit
CCTGTTTGCTACCNATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA760.3151957531519575No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCNAGCA750.31104844061048437No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAANGTCAGTTTCAGTNNAGTA750.31104844061048437No Hit
CCTGTTTGCTCCCNACACTTTCGTGCCTCAACGTCAGTTTCAGTNCAGTA700.2903118779031188No Hit
CCTGTTTGCTCCCNACACTTTCGTGCNTCAANGTNAGTTACNGTNCAGAA690.28616456536164564No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA660.2737226277372263No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTNAGTTAAAGCCCAGCA660.2737226277372263No Hit
CCTGTTTGCTCCCCACACTTTNGTGCCTCAACGTCAGTTTCAGTCCAGTA660.2737226277372263No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNCAGTA650.2695753151957532No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTNAACGTCAGTTACTGTNCAGAA650.2695753151957532No Hit
CCTGTTTGCTACCNATGCTTTCGAGCNTCAGNGTNAGTTGGNGCNCAGTA650.2695753151957532No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTGCTGTCCAGCA620.25713337757133375No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACGGTNCAGAA610.25298606502986065No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTNCAGTA590.24469143994691442No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACGGTNNAGAA590.24469143994691442No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTNGGTA580.2405441274054413No Hit
CNTGTTTGCTCCCCACACTTTCGTGCCTCAACGTNAGTTTCAGTCCAGTA550.22810218978102187No Hit
CCTGTTTGCNCCCCACACTTTCGTGCCTCAACGTCAGTTTCAGTNCAGTA550.22810218978102187No Hit
CCTGTTNGCTCCCCACACTTTCGTGCCTCAACGTCAGTTTCAGTCCAGTA540.2239548772395488No Hit
CCTGTTTGCTCCCNACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNCAGCA530.21980756469807566No Hit
CCTGTTTGCTCCCNACACTTTCGTGCNTCAANGTNAGTTTCNGTNCAGTA530.21980756469807566No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCNNAGAG520.21566025215660253No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTNNAGTA520.21566025215660253No Hit
CNTGTTTGCTCCCCACACTTTCGTGCCTCAACGTNAGTTACTGTCCAGAA510.2115129396151294No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTATTGTCCAGAA510.2115129396151294No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTNNGGTA500.20736562707365627No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAANGTCAGTTACTGTNNAGAA500.20736562707365627No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTNAGTTGTCGTCCAGCA500.20736562707365627No Hit
CCTGTTTGCTACCCACGCTTTNGTGCCTCAGCGTCAGTTAAAGCCCAGCA490.20321831453218314No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCNAGTA480.19907100199071004No Hit
CCTGTTTGCTCCCCACGCTTTNGCGCCTCAGCGTCAGTTGTCGTCCAGCA480.19907100199071004No Hit
CCTGTTNGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTNCAGAA480.19907100199071004No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTNAGNTACTGTCCAGAA480.19907100199071004No Hit
CCTGTTTGCNACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCNCAGCA480.19907100199071004No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTNAGNTAAAGCCCAGCA470.19492368944923688No Hit
CCTGTTNGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNCAGCA470.19492368944923688No Hit
CCTGTTTGCTCCCCACACTTTNGTGCCTCAACGTCAGTTACTGTCCAGAA470.19492368944923688No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGNGTCAGTTGTCGTNNAGCA470.19492368944923688No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTNTGGTA470.19492368944923688No Hit
CCTGTTNGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA460.19077637690776378No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCNAGTA460.19077637690776378No Hit
CCTGTTTGCNCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTNCAGAA450.18662906436629065No Hit
CCTGTTNGCTCCCCACACTTTCGTGCCTCAACGTCAGTTTCAGTNCAGTA450.18662906436629065No Hit
CCTGTTTGCTCCCNACGCTTTCGCGCNTCAGNGTNAGTTGTNGTNCAGCA450.18662906436629065No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCNAGAG450.18662906436629065No Hit
CCTGTTTGCTACCNACGCTTTCGTGCNTCAGNGTNAGTTAANGCNCAGCA440.18248175182481752No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNCAGTA430.17833443928334441No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGNGTCAGTTAAAGCNNAGCA430.17833443928334441No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTNAGNTTCAGTCCAGTA430.17833443928334441No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGNTGTCGTCCAGCA430.17833443928334441No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTNAACGTCAGTTTCAGTNCAGTA430.17833443928334441No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTNAGCGTCAGTTGTCGTNCAGCA420.17418712674187126No Hit
CCTGTTNGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGAA410.17003981420039813No Hit
CNTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA410.17003981420039813No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNNAGTA400.16589250165892502No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCNCAGAG400.16589250165892502No Hit
CCTGTTTGCTCCCNACACTTTCGTGCCTCAACGTCAGTTACTGTNCAGAA390.1617451891174519No Hit
CNTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTNAGTTAAAGCCCAGCA390.1617451891174519No Hit
CCTGTTTGCTACCNACGCTTTCGTGCCTCAGCGTCAGTTAAAGCNCAGCA390.1617451891174519No Hit
CCTGTTTGCNCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTNCAGCA380.15759787657597876No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNCAGCA380.15759787657597876No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCNNAGTT380.15759787657597876No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA380.15759787657597876No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTNACCGTCAGTTGTCGTNCAGCA360.14930325149303253No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTNAGCGTCAGTTAAAGCNCAGCA360.14930325149303253No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA360.14930325149303253No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTNNAGAA350.1451559389515594No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTNAGCA350.1451559389515594No Hit
CCTGTTNGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCNCAGCA350.1451559389515594No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCNCAGTT340.14100862641008627No Hit
CCTGTTNGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA340.14100862641008627No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCNAGTT340.14100862641008627No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTNAGATATAGTTTGGTA330.13686131386861314No Hit
CCTGTTNGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTNCAGCA330.13686131386861314No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCNAGCA320.13271400132714No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNNAGTA320.13271400132714No Hit
CCTGTTTGCTCCCNACGCTTTCGCGCNTCACNGTNAGTTGTNGTNCAGCA320.13271400132714No Hit
CNTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGTTGTCGTCCAGCA310.12856668878566688No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTGCAGTCCAGAA310.12856668878566688No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTNAGTGACGGCCCAGAG310.12856668878566688No Hit
CCTGTTCGCTACCCACGCTCTCGTGCATCAGCGTCAGTTACAGTCTGGTA310.12856668878566688No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTNNAGTA300.12441937624419377No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNNAGCA300.12441937624419377No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCNAGAA300.12441937624419377No Hit
CCTGTTTGCTACCNACACTTTCGAGCNTCAGNGTNAGTTGGNGCNCAGTA300.12441937624419377No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCNAGTA290.12027206370272064No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACNGTCAGTTGTCGTNNAGCA290.12027206370272064No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA290.12027206370272064No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTNAACGTCAGATATAGTNTGGTA280.1161247511612475No Hit
CCTGTTTGCTCCCCACGCTTTNGCGCCTCACCGTCAGTTGTCGTCCAGCA280.1161247511612475No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTNTAGTA280.1161247511612475No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG280.1161247511612475No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTAACAGTCTAGTG280.1161247511612475No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTATCGTCCAGTA280.1161247511612475No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNNAGCA280.1161247511612475No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGNTGGTGCCCAGTA270.1119774386197744No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTNAGTAGCAGTCCAGTA270.1119774386197744No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGTTGCAGCTTAGCA270.1119774386197744No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTCAGTCCAGAA270.1119774386197744No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTATTGTCNAGAA270.1119774386197744No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTGCTGTCNAGCA270.1119774386197744No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCA260.10783012607830127No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTGCTGTNCAGCA260.10783012607830127No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA260.10783012607830127No Hit
CCTGTTTGCTCCCCACGCTTTNGAGCCTCAACGTCAGTTACCGTCCAGTA250.10368281353682814No Hit
CCTGTTTGCTACCCACACTTTNGAGCCTCAGCGTCAGTTGGTGCCCAGTA250.10368281353682814No Hit
CCTGTTTGCNCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTNCAGCA250.10368281353682814No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTT250.10368281353682814No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTNAGNTATAGTTTGGTA250.10368281353682814No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTNAGTTACAGTCCAGCA250.10368281353682814No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTNAGTTACGGTCCAGAA250.10368281353682814No Hit
CCTGTTNGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA250.10368281353682814No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)