FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002450719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002450719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57323
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA19393.3825863963853955No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA18833.284894370497008No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA10581.8456814891055946No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA7671.3380318545784415No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACAGTTTAGCA7521.3118643476440521No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA7201.2560403328506882No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNNAGTA6521.1374143014147897No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCNAGTA6441.123458297716449No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA6401.1164802958672784No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA6371.1112467944804005No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCGCTCCCGTC4720.8234042182021178No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA4500.7850252080316802No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA4440.7745582052579244No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTNCAGTA4060.7082671876908048No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTNNAGTA4020.7012891858416342No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCNAGTA3890.6786106798318301No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCA3550.619297664113881No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAACGTCAGTGGCGGCTTAGCA3170.5530066465467613No Hit
CCCGTTCGCTCCCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA3140.5477731451598834No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGAA2990.5216056382254941No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGNGTCAGTTATCGTNNAGTA2500.4361251155731556No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGCTTAGTG2490.434380615110863No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA2470.4308916141862778No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA2450.42740261326169254No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACAGTTNAGCA2440.42565811279939986No Hit
CCTGTTTGCNACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA2440.42565811279939986No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTAATCGTCCAGTA2390.4169356104879368No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACAGTNNAGCA2390.4169356104879368No Hit
CCTGTTTGCTACCCACACTTTNGAGCCTCAGCGTCAGTTGGTGCCCAGTA2380.41519111002564413No Hit
CCTGTTTGCTACCNACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA2380.41519111002564413No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACAGTNTAGCA2340.40821310817647366No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA2330.4064686077141811No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAGNGTNAGTTATNGTNCAGTA2300.4012351063273032No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGNGTCAGTTGGTGCNNAGTA2280.39774610540271793No Hit
CCTGTTTGCTCCCCACGCTTTNGAGCCTCAGCGTCAGTTATCGTCCAGTA2280.39774610540271793No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTNAGCGTCAGTTATCGTNCAGTA2260.39425710447813267No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA2220.3872791026289622No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGNTGGTGCCCAGTA2210.38553460216666957No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA2210.38553460216666957No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTNAGCGTCAGTTGGTGCNCAGTA2200.38379010170437694No Hit
CCTGTTTGCTACCNACACTTTCGAGCNTCAGNGTNAGTTGGNGCNCAGTA2170.3785566003174991No Hit
CNTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA2160.3768120998552065No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGNTATCGTCCAGTA2150.37506759939291384No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA2130.3715785984683286No Hit
CCTGTTTGCNCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA2080.3628560961568655No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA1960.341922090609354No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCCAGTA1860.32447708598642777No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA1860.32447708598642777No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCGCTNNCGTC1770.3087765818257942No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCGCTNCCGTC1670.29133157720286795No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTNAGTTACAGTTTAGCA1650.28784257627828275No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA1630.28435357535369743No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCGCTCNCGTC1600.2791200739668196No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1600.2791200739668196No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA1590.27737557350452696No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCNNAGCA1540.26865307119306386No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGNGTCAGTAATCGTNNAGTA1450.25295256703243024No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCCTCAGCGTCAGTAATCGTNCAGTA1450.25295256703243024No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA1440.2512080665701376No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA1390.2424855642586745No Hit
CCTGTTTGCTCCCCACGCTTTNGAGCCTCAGCGTCAGTAATCGTCCAGTA1350.23550756240950402No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAGNGTNAGTAATNGTNCAGTA1300.22678506009804092No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTAATCGTCCAGTA1290.2250405596357483No Hit
CCTGTTTGCNCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTNCAGTA1270.22155155871116305No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTNNAGAA1270.22155155871116305No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCNCAGCA1260.21980705824887042No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAACGTCAGTGGCGGCNTAGCA1260.21980705824887042No Hit
CCCGTTCGCTCCCCTGGCTTTCGCATCTCAGCGTCAGACACAGTNNAGAA1260.21980705824887042No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCNAGCA1230.21457355686199256No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTNAGCGTCAGTAATCGTNCAGTA1220.21282905639969993No Hit
CCCGTTCGCTCCCCTGGCTTTCGCATCTCAGCGTCAGACACAGTNCAGAA1170.20410655408823683No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA1150.2006175531636516No Hit
CCCGTTCGCTCCCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCNAGAA1140.19887305270135897No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTNAGTTAGAGCCCAGCA1120.19538405177677373No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA1090.19015055038989587No Hit
CCTGTTCGATACCCGCACCTTCGAGCATCAGCGTCAGTAATGCTGCGGCA1090.19015055038989587No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAACGTCAGTGGCGGCNNAGCA1080.18840604992760324No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTNCAGTA1080.18840604992760324No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTNCAGAA1080.18840604992760324No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTNAGTTGCGCTCCCGTC1070.1866615494653106No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTGGCGGTTTAGCA1070.1866615494653106No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGNAATCGTCCAGTA1070.1866615494653106No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCNAGAA1060.18491704900301797No Hit
CCTGTTNGATACCCACACTTTCGAGCATGAACGTCAGTTACAGTTTAGCA1050.18317254854072537No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTACAGCTTAGCA1050.18317254854072537No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAACGTCAGTGGCGGCTNAGCA1020.1779390471538475No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGCA1010.17619454669155488No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA980.170961045304677No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA970.16921654484238438No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTNGGTA940.16398304345550652No Hit
CNTGTTTGATACCCACACTTTCGAGCATGAACGTNAGTTACAGTTTAGCA940.16398304345550652No Hit
CCTGTTTGANACCCACACTTTCGAGCATGAACGTCAGTTACAGTNTAGCA930.16223854299321389No Hit
CCTGTTNGATACCCACACTTTCGAGCATGAACGTCAGTTACAGTNTAGCA910.15874954206862865No Hit
CCTGTTTGATACCNACACTTTCGAGCATGAACGTCAGTTACAGTNTAGCA900.15700504160633602No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTNAGNTACAGTTTAGCA870.15177154021945816No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGCNTAGTG870.15177154021945816No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAANGTCAGTTACAGTNNAGCA860.15002703975716555No Hit
CCTGTTTGATACCCACACTTTCGAGCATNAACGTCAGTTACAGTNTAGCA850.14828253929487292No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTNTGGTA840.1465380388325803No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAACGTNAGTGGCGGCTTAGCA830.1447935383702877No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGCTNAGTG820.14304903790799506No Hit
CCTGTTTGATACCCACACTTTNGAGCATGAACGTCAGTTACAGTTTAGCA800.1395600369834098No Hit
CCTGTTTGATACCNACACTTTCGAGCNTGAANGTNAGTTACNGTNTAGCA790.13781553652111717No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTNNGGTA780.13607103605882453No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTNAGATATAGTTTGGTA760.1325820351342393No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA760.1325820351342393No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGCNNAGTG740.12909303420965407No Hit
CCCGTTCGCTCCCCTGGCTTTCGCATCTCAGCGTNAGACACAGTCCAGAA730.12734853374736144No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTNAGNTGCGCTCCCGTC730.12734853374736144No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTNNAGTA720.1256040332850688No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNNAGCA700.12211503236048357No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAGTTGGGCGCCGGTG680.11862603143589832No Hit
CCCGTTTGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA680.11862603143589832No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTNNAGTA680.11862603143589832No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCTAGAA670.11688153097360571No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCNAGCA660.11513703051131308No Hit
CNTGTTCGATACCCGCACTTTCGAGCCTCAGCGTNAGTTGCGCTCCCGTC660.11513703051131308No Hit
CCTGTTNGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCGCTNCCGTC650.11339253004902046No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCNNAGTA640.11164802958672784No Hit
CCTGTTCGANACCCGCACTTTCGAGCCTCAGCGTCAGTTGCGCTNCCGTC640.11164802958672784No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGNGTCAGTTGCGCTNNCGTC640.11164802958672784No Hit
CCTGTTCGATACCCGCACCTTCGAGCATCAGCGTCAGTAATGCTNCGGCA630.10990352912443521No Hit
CCTGTTCGATACCCGCACCTTCGAGCATCAGCGTCAGTAATGCTNNGGCA630.10990352912443521No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATNNAGTA630.10990352912443521No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA620.1081590286621426No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCNCAGTA610.10641452819984996No Hit
CCTGTTCGATACCNGCACTTTCGAGCNTCAGNGTNAGTTGCNCTNCCGTC610.10641452819984996No Hit
CCTGTTCGATACCNGCACTTTCGAGCCTCAGCGTCAGTTGCGCTNCCGTC610.10641452819984996No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA610.10641452819984996No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCNAGTA600.10467002773755735No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCNAGTA600.10467002773755735No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA590.10292552727526473No Hit
CCTGTTCGATACCCGCACTTTNGAGCCTCAGCGTCAGTTGCGCTCCCGTC580.1011810268129721No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)