FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002450731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002450731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26670
Sequences flagged as poor quality0
Sequence length301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCCAGTA10663.997000374953131No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA10203.824521934758155No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA6542.4521934758155233No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATNCAGTA4171.5635545556805397No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATNNAGTA4121.5448068991376078No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCNAGTA4031.5110611173603299No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNNAGCA3981.4923134608173978No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTNAGCA3311.2410948631421073No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA3161.1848518935133108No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTNAGTTACAATCCAGTA2831.0611173603299586No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA2671.001124859392576No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA2660.9973753280839894No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGTTGCAGCTTAGCA2590.9711286089238845No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA2390.8961379827521561No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA2220.8323959505061868No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA2170.8136482939632546No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA2150.8061492313460819No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA1790.6711661042369704No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGTTAGTA1550.5811773528308962No Hit
CCTGTTTGATACCNACACTTTCGAGCATCAGCGTCAGTTACAATNCAGTA1540.5774278215223096No Hit
CCTGTTTGATACCNACACTTTCGAGCNTCAGNGTNAGTTACNATNCAGTA1530.5736782902137233No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTNAGNTACAATCCAGTA1470.5511811023622047No Hit
CCTGTTTGATACCCACACTTTCGAGCATNAGCGTCAGTTACAATNCAGTA1460.5474315710536183No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGNTGCAGCTTAGCA1380.5174353205849269No Hit
CNTGTTTGATACCCACACTTTCGAGCATCAGCGTNAGTTACAATCCAGTA1380.5174353205849269No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA1370.5136857892763405No Hit
CCTGTTNGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA1340.5024371953505812No Hit
CCTGTTTGATACCNACACTTTCGAGCNTCAANGTNAGTTGCNGCNTAGCA1330.4986876640419947No Hit
CCTGTTTGATACCNACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA1310.4911886014248219No Hit
CCTGTTTGATACCCACACTTTNGAGCATCAGCGTCAGTTACAATCCAGTA1310.4911886014248219No Hit
CCTGTTNGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATNCAGTA1280.4799400074990626No Hit
CCTGTTNGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCCAGTA1270.4761904761904762No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGNGTCAGTTACAATNNAGTA1260.47244094488188976No Hit
CNTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGTTGCAGCTTAGCA1250.46869141357330335No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG1210.4536932883389576No Hit
CCTGTTNGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA1200.44994375703037126No Hit
CCTGTTTGANACCCACACTTTCGAGCATCAGCGTCAGTTACAATNCAGTA1180.4424446944131984No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTNNAGCA1130.4236970378702662No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA1120.4199475065616798No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1100.4124484439445069No Hit
CCTGTTTGATACCCACACTTTCGAGCCTNAATGTCAGTTGCAGCNTAGCA1090.4086989126359205No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTNCAGCA1080.40494938132733405No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAANGTCAGTTGCAGCNNAGCA1070.40119985001874764No Hit
CCTGTTTGANACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA1070.40119985001874764No Hit
CCTGTTTGATACCCACACTTTNGAGCCTCAATGTCAGTTGCAGCTTAGCA1060.3974503187101612No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTNNAGAA1020.38245219347581555No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA1020.38245219347581555No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG1010.3787026621672291No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCNAGTA970.3637045369328834No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNNAGTA970.3637045369328834No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGNGTCAGTTGGTGCNNAGTA960.35995500562429694No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCNAGCA910.34120734908136485No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGCA880.3299587551556056No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTNCAGAA850.31871016122984624No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCTAGTG840.31496062992125984No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCNAGAA800.29996250468691416No Hit
CCTGTTTGCNACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA760.2849643794525684No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTNNAGTA750.281214848143982No Hit
CNTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA750.281214848143982No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA740.2774653168353956No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGNTGGTGCCCAGTA730.27371578552680914No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTNNAGTG710.26621672290963627No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA700.26246719160104987No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTACAATCCAGTA690.25871766029246346No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTNAGCGTCAGTTGGTGCNCAGTA680.254968128983877No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGNNAGTA680.254968128983877No Hit
CCTGTTTGCTACCNACACTTTCGAGCNTCAGNGTNAGTTGGNGCNCAGTA660.24746906636670418No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCNAGTA650.24371953505811775No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCNAGTG650.24371953505811775No Hit
CCTGTTTGCTACCNACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA650.24371953505811775No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA640.2399700037495313No Hit
CCTGTTTGCTACCCACACTTTNGAGCCTCAGCGTCAGTTGGTGCCCAGTA630.23622047244094488No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCCTAGCA610.228721409823772No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTNAGTTACAGTCCAGCA610.228721409823772No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTNAGTTACAGGTTAGTA600.22497187851518563No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGTTTTCGTCCAGAA600.22497187851518563No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTA570.21372328458942633No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGTNAGTA550.20622422197225346No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTNCAGTA540.20247469066366702No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCNNAGAG540.20247469066366702No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCNAGAG540.20247469066366702No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCATGTCCCAGAG490.1837270341207349No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA490.1837270341207349No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNNAGTA470.17622797150356206No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNCAGTA470.17622797150356206No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCNAGTA460.17247844019497563No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAANGTNAGTTACNGTNCAGTA460.17247844019497563No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGNTAGTA440.1649793775778028No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTNCAGTG430.16122984626921635No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTNAGNTACAGTCCAGCA430.16122984626921635No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGNGTCAGTTTTCGTNNAGAA430.16122984626921635No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA420.15748031496062992No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTNAGCGTCAGTTATCGTNCAGTA400.14998125234345708No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA400.14998125234345708No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAGNGTNAGTTACNGTNCAGCA390.14623172103487064No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA380.1424821897262842No Hit
CCTGTTNGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA380.1424821897262842No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCNCAGAG370.1387326584176978No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGTTACTGTCCAGTA370.1387326584176978No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA360.13498312710911137No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCNNGGCA360.13498312710911137No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA350.13123359580052493No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA350.13123359580052493No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCNTGGCA350.13123359580052493No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA350.13123359580052493No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCCTCAGTGTCAGTTACAGTNCAGCA350.13123359580052493No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA340.1274840644919385No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTNAGCGTCAGTTTTCGTNCAGAA340.1274840644919385No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA330.12373453318335209No Hit
CCTGTTTGCTCCCCACGCTTTNGAGCCTCAGCGTCAGTTATCGTCCAGTA330.12373453318335209No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCCNAGCA330.12373453318335209No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA330.12373453318335209No Hit
CNTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGTTTTCGTCCAGAA320.11998500187476566No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTNAGTGTCAGTTACAGTNCAGCA320.11998500187476566No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTNGGCA320.11998500187476566No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAGNGTNAGTTATNGTNCAGTA310.11623547056617922No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGNGTCAGTTATCGTNNAGTA310.11623547056617922No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA310.11623547056617922No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGNTATCGTCCAGTA310.11623547056617922No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA300.11248593925759282No Hit
CCTGTTTGATACCNACACTTTCGAGCNTCAANGTNAGTTACNGGNTAGTA300.11248593925759282No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGTTACCGTCCAGTA300.11248593925759282No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGNTACTGTCCAGTA300.11248593925759282No Hit
CCTGTTTGATACCCACACTTTNGAGCATCAATGTCAGTTACAGGTTAGTA300.11248593925759282No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA290.10873640794900638No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTNAGTTGCAGTCCAGTG290.10873640794900638No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTNAGTTACAGTCCAGCA290.10873640794900638No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTNCAGCA290.10873640794900638No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTNAGTGACGGCCCAGAG290.10873640794900638No Hit
CCTGTTTGCNCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTNCAGCA280.10498687664041995No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGTA280.10498687664041995No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGNGTCAGTTACAGTNNAGCA270.10123734533183351No Hit
CCTGTTTGCTCCCNACGCTTTCGCGCNTCAGNGTNAGTTTTNGTNCAGAA270.10123734533183351No Hit
CCTGTTTGCTCCCCACGCTTTNGCGCCTCAGCGTCAGTTTTCGTCCAGAA270.10123734533183351No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)