FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002450733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002450733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14253
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA5193.6413386655440956No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCCAGTA4523.1712621904160527No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA3822.6801375149091418No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA2211.5505507612432472No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA2011.4102294253841297No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA1841.29095628990388No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA1791.2558759559391004No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATNCAGTA1731.2137795551813653No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1721.2067634883884095No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1541.0804742861152037No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCNAGTA1481.0383778853574686No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATNNAGTA1461.0243457517715568No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA1441.0103136181856451No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA1400.9822493510138216No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNNAGCA1340.9401529502560865No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1160.8138637479828807No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA1100.7717673472251456No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTNAGCA1070.7507191468462779No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGTTGCAGCTTAGCA1070.7507191468462779No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTNAGTTACAATCCAGTA1040.7296709464674104No Hit
CCTGTTTGCTACCNACACTTTCGAGCNTCAGNGTNAGTTGGNGCNCAGTA860.6033817441942048No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGNTGGTGCCCAGTA840.5893496106082929No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGTA830.5823335438153371No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNNAGTA810.5683014102294254No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA800.5612853434364695No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA780.5472532098505577No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTNNAGCA750.5262050094716901No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCTAGTG750.5262050094716901No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCNAGTA700.4911246755069108No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCNAGCA690.48410860871395495No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTNCAGCA680.4770925419209991No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTNAGNTACAATCCAGTA670.47007647512804324No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA660.4630604083350874No Hit
CCTGTTTGCTACCNACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA660.4630604083350874No Hit
CCTGTTTGATACCCACACTTTNGAGCCTCAATGTCAGTTGCAGCTTAGCA660.4630604083350874No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTNNAGAA650.45604434154213147No Hit
CCTGTTTGATACCNACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA650.45604434154213147No Hit
CCTGTTTGATACCNACACTTTCGAGCATCAGCGTCAGTTACAATNCAGTA640.4490282747491756No Hit
CCTGTTTGATACCCACACTTTCGAGCATNAGCGTCAGTTACAATNCAGTA630.44201220795621976No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGNGTCAGTTGGTGCNNAGTA630.44201220795621976No Hit
CNTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA630.44201220795621976No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTNCAGAA610.42798007437030805No Hit
CCTGTTTGATACCNACACTTTCGAGCNTCAGNGTNAGTTACNATNCAGTA610.42798007437030805No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG600.4209640075773522No Hit
CCTGTTNGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCCAGTA590.41394794078439623No Hit
CCTGTTNGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATNCAGTA560.39289974040552866No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTNAGCGTCAGTTGGTGCNCAGTA560.39289974040552866No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA550.3858836736125728No Hit
CCTGTTTGCNACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA540.3788676068196169No Hit
CCTGTTTGATACCNACACTTTCGAGCNTCAANGTNAGTTGCNGCNTAGCA540.3788676068196169No Hit
CCTGTTTGCTACCCACACTTTNGAGCCTCAGCGTCAGTTGGTGCCCAGTA520.3648354732337052No Hit
CCTGTTTGATACCCACACTTTCGAGCCTNAATGTCAGTTGCAGCNTAGCA520.3648354732337052No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA510.3578194064407493No Hit
CCTGTTTGATACCCACACTTTNGAGCATCAGCGTCAGTTACAATCCAGTA510.3578194064407493No Hit
CNTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGTTGCAGCTTAGCA510.3578194064407493No Hit
CCTGTTTGANACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA510.3578194064407493No Hit
CCTGTTTGANACCCACACTTTCGAGCATCAGCGTCAGTTACAATNCAGTA500.3508033396477934No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA500.3508033396477934No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA470.32975513926892586No Hit
CCTGTTNGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA470.32975513926892586No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGNGTCAGTTACAATNNAGTA460.32273907247597No Hit
CNTGTTTGATACCCACACTTTCGAGCATCAGCGTNAGTTACAATCCAGTA460.32273907247597No Hit
CCTGTTNGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA460.32273907247597No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA450.3157230056830141No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAANGTCAGTTGCAGCNNAGCA440.30870693889005824No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGNTGCAGCTTAGCA430.3016908720971024No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCNAGAA430.3016908720971024No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTNNAGTG410.2876587385111906No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTNAGTTACAGTCCAGCA410.2876587385111906No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTNCAGTA380.266610538132323No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG370.25959447133936714No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA370.25959447133936714No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCNNAGAG370.25959447133936714No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTNNAGTA360.2525784045464113No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGTTAGTA350.2455623377534554No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCNAGTG340.23854627096049955No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCNAGAG340.23854627096049955No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTA330.2315302041675437No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCNAGTA320.2245141373745878No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGNGTCAGTTATCGTNNAGTA310.21749807058163195No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTNTAGTG310.21749807058163195No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGTTTTCGTCCAGAA310.21749807058163195No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCCTAGCA300.2104820037886761No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAGNGTNAGTTATNGTNCAGTA280.19644987020276433No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTNAGTTACAGTCCAGCA280.19644987020276433No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA270.18943380340980845No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA270.18943380340980845No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTNAGNTACAGTCCAGCA270.18943380340980845No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAANGTNAGTTACNGTNCAGTA260.1824177366168526No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNCAGTA260.1824177366168526No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA260.1824177366168526No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA250.1754016698238967No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCNCAGAG250.1754016698238967No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAGNGTNAGTTACNGTNCAGCA240.16838560303094086No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCATGTCCCAGAG240.16838560303094086No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGNTATCGTCCAGTA240.16838560303094086No Hit
CNTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA240.16838560303094086No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCNAGTA230.161369536237985No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNNAGTA230.161369536237985No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA230.161369536237985No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTNAGTGACGGCCCAGAG230.161369536237985No Hit
CCTGTTTGCTCCCNACGCTTTCGCGCCTCAGCGTCAGTTTTCGTNCAGAA220.15435346944502912No Hit
CCTGTTTGCTCCCCACGCTTTNGCGCCTCAGCGTCAGTTTTCGTCCAGAA220.15435346944502912No Hit
CCTGTTTGCTCCCCACGCTTTNGAGCCTCAGCGTCAGTTATCGTCCAGTA220.15435346944502912No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTNCAGTA220.15435346944502912No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGNTTTCGTCCAGAA220.15435346944502912No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA210.14733740265207323No Hit
CCTGTTTGCTCCCNACGCTTTCGCGCNTCAGNGTNAGTTTTNGTNCAGAA210.14733740265207323No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGNGTCAGTTGCAGTNNAGTG210.14733740265207323No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGCA210.14733740265207323No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTNTAGTA210.14733740265207323No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA210.14733740265207323No Hit
CCTGTTNGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA200.14032133585911738No Hit
CCTGTTTGCTCCCCACGCTTTNGAGCCTCAGTGTCAGTTACAGTCCAGCA200.14032133585911738No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTNCAGTA200.14032133585911738No Hit
CCTGTTNGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTNCAGAA200.14032133585911738No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAANGTCAGTTACTGTNNAGTA190.1333052690661615No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTNAGCGTCAGTTATCGTNCAGTA190.1333052690661615No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTNAGCGTCAGTTTTCGTNCAGAA190.1333052690661615No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTNAGTGTCAGTTACAGTNCAGCA190.1333052690661615No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCCTCAGTGTCAGTTACAGTNCAGCA190.1333052690661615No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTNNAGTA180.12628920227320564No Hit
CCTGTTTGCNCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA180.12628920227320564No Hit
CNTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTNAGTTTTCGTCCAGAA180.12628920227320564No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTNAGTTGCAGTCTAGTG180.12628920227320564No Hit
CCTGTTTGCTCCCCACGCTTTNGAGCCTCAACGTCAGTTACTGTCCAGTA180.12628920227320564No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA180.12628920227320564No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCNAGTA170.11927313548024977No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGNTGGTGCCCAGTA170.11927313548024977No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTNCAGCA170.11927313548024977No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGNTACTGTCCAGTA170.11927313548024977No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGNGTCAGTTTTCGTNNAGAA160.1122570686872939No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTNAGCGTCAGTTGGTGCNCAGTA160.1122570686872939No Hit
CCTGTTNGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA160.1122570686872939No Hit
CCTGTTTGCNCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTNCAGCA160.1122570686872939No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGNTAGTA160.1122570686872939No Hit
CCTGTTTGCTACCCATGCTTTNGAGCCTCAGCGTCAGTTGGTGCCCAGTA160.1122570686872939No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGTTACTGTCCAGTA160.1122570686872939No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTNAGTTATGGTTTAGTA160.1122570686872939No Hit
CCTGTTNGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA150.10524100189433805No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTACAATCCAGTA150.10524100189433805No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA150.10524100189433805No Hit
CCTGTTTGCTCCCCACGCTTTNGAGCCTCAGCGTCAGTTACAGTCCAGTA150.10524100189433805No Hit
CCTGTTTGCNCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTNCAGAA150.10524100189433805No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)