FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002450739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002450739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32711
Sequences flagged as poor quality0
Sequence length301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA15844.842407752743726No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA9893.0234477698633486No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNNAGCA5841.7853321512641007No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA5541.693619883219712No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTNAGCA5151.5743939347620066No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA3961.2106019381859314No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA3891.189202408975574No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGTTGCAGCTTAGCA3791.1586316529607776No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA3481.0638623093149093No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTGACAGTCTAGTG3461.05774815811195No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA3100.9476934364586835No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA3040.9293509828498059No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA2760.8437528660083763No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA2500.7642689003699061No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGTTAGTA2300.7031273883403136No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA2290.700070312738834No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAANGTCAGTTGCAGCNNAGCA2080.6358717251077619No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA2060.6297575739048027No Hit
CCTGTTTGATACCNACACTTTCGAGCNTCAANGTNAGTTGCNGCNTAGCA1950.5961297422885268No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGNTGCAGCTTAGCA1870.5716731374766898No Hit
CCTGTTTGANACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA1830.5594448350707713No Hit
CCTGTTTGATACCNACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA1790.5472165326648528No Hit
CNTGTTTGATACCCACACTTTCGAGCCTCAATGTNAGTTGCAGCTTAGCA1780.5441594570633731No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG1770.5411023814618936No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA1740.5319311546574547No Hit
CCTGTTTGATACCCACACTTTCGAGCCTNAATGTCAGTTGCAGCNTAGCA1740.5319311546574547No Hit
CCTGTTNGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCNTAGCA1710.5227599278530158No Hit
CCTGTTTGATACCCACACTTTNGAGCCTCAATGTCAGTTGCAGCTTAGCA1700.5197028522515362No Hit
CCTGTTNGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA1460.4463330378160252No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNNAGTA1430.43716181101158635No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCNAGTA1390.4249335086056678No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG1360.415762281801229No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1330.4065910549967901No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA1320.40353397939531044No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTGACAGTNNAGTG1260.38519152578643273No Hit
CCTGTTTGCTACCNACACTTTCGAGCNTCAGNGTNAGTTGGNGCNCAGTA1260.38519152578643273No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGNGTCAGTTGGTGCNNAGTA1210.3699061477790346No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTGACAGTNTAGTG1210.3699061477790346No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA1210.3699061477790346No Hit
CCTGTTTGCTACCCACACTTTNGAGCCTCAGCGTCAGTTGGTGCCCAGTA1200.36684907217755497No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTGACAGTCNAGTG1200.36684907217755497No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNNAGTA1190.36379199657607536No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGNTGGTGCCCAGTA1140.3485066185686772No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1140.3485066185686772No Hit
CCTGTTNGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1130.34544954296719754No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA1130.34544954296719754No Hit
CCTGTTTGCTACCNACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA1130.34544954296719754No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA1100.3362783161627587No Hit
CNTGTTTGCTACCCACACTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA1080.33016416495979944No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNCAGCA1030.31487878695240135No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCNAGTA1020.31182171135092174No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCNAGCA910.2781938797346458No Hit
CCTGTTTGCNACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA910.2781938797346458No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNNAGTA910.2781938797346458No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTNAGCGTCAGTTGGTGCNCAGTA910.2781938797346458No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNNAGCA880.26902265293020694No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTNCAGTA860.2629085017272477No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA830.25373727492280884No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGNTAGTA820.25068019932132923No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCNAGTA810.24762312371984957No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCNAGCA790.24150897251689032No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGNNAGTA760.23233774571245147No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGTNAGTA750.22928067011097186No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGTTGGTGCCCAGTA740.2262235945094922No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTNAGTTACAGTCCAGCA740.2262235945094922No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTNNAGCA740.2262235945094922No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCCTAGCA720.22010944330653298No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTNAGTGACAGTCTAGTG720.22010944330653298No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA700.21399529210357374No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTNCAGAA700.21399529210357374No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTNCAGTG670.20482406529913483No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGCA670.20482406529913483No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCA660.20176698969765522No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA660.20176698969765522No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGTCCAGTA660.20176698969765522No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTNCAGCA660.20176698969765522No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG640.19565283849469597No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCNCAGAG630.19259576289321637No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTNNAGAA610.1864816116902571No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA610.1864816116902571No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTNAGTTACAGGTTAGTA600.18342453608877748No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCNAGAA580.17731038488581824No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCNAGTG580.17731038488581824No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTNNAGTG560.17119623368285897No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGTTACCGTCCAGTA560.17119623368285897No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCNAGAG560.17119623368285897No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA560.17119623368285897No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCNNAGAG540.16508208247989972No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTA540.16508208247989972No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGTA500.15285378007398123No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTNAGTA500.15285378007398123No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGATGCCCAGTA490.1497967044725016No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTNAGNGACAGTCTAGTG490.1497967044725016No Hit
CNTGTTTGATACCCACACTTTCGAGCATCAGTGTNAGTGACAGTCTAGTG480.14673962887102196No Hit
CCTGTTTGANACCCACACTTTCGAGCATCAGTGTCAGTGACAGTNTAGTG470.14368255326954238No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTNGGTA460.14062547766806274No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGNGTCAGTTATCGTNNAGTA450.1375684020665831No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAANGTNAGTTACNGTNCAGTA450.1375684020665831No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTGTCGTCNAGAA440.13451132646510347No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTNAGTTACACTCCAGTG440.13451132646510347No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGNGTCAGTTGGTGCNNAGTA440.13451132646510347No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGNGTCAGTGACAGTNNAGTG430.13145425086362386No Hit
CCTGTTNGATACCCACACTTTCGAGCATCAGTGTCAGTGACAGTCTAGTG430.13145425086362386No Hit
CCTGTTTGCTACCNATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA420.12839717526214423No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTNAGATATAGTTTGGTA410.12534009966066462No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCCNAGCA410.12534009966066462No Hit
CCTGTTTGATACCCACACTTTCGAGCATNAGTGTCAGTGACAGTNTAGTG410.12534009966066462No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTNAGCGTCAGTTATCGTNCAGTA410.12534009966066462No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTNAGACACAGTCCAGAA410.12534009966066462No Hit
CCTGTTTGATACCCACACTTTCGAGCATNAGCGTCAGTTACAGTNCAGCA410.12534009966066462No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTNTGGTA410.12534009966066462No Hit
CCTGTTNGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTNCAGCA400.122283024059185No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTNNGGTA390.11922594845770536No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTGTCGTNNAGAA390.11922594845770536No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCNAGTA380.11616887285622574No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTNTAGTA380.11616887285622574No Hit
CCTGTTTGATACCCACACTTTNGAGCATCAGTGTCAGTGACAGTCTAGTG380.11616887285622574No Hit
CCTGTTNGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA380.11616887285622574No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTNAGCGTCAGTTGGTGCNCAGTA370.1131117972547461No Hit
CCTGTTTGATACCNACACTTTCGAGCATCAGCGTCAGTTACAGTNCAGCA370.1131117972547461No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAANGTCAGTTACAGGNNAGTA370.1131117972547461No Hit
CCTGTTTGATACCNACACTTTCGAGCATCAGTGTCAGTGACAGTNTAGTG370.1131117972547461No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA370.1131117972547461No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTNAGNTGGTGCCCAGTA370.1131117972547461No Hit
CCTGTTTGATACCNACACTTTCGAGCNTCAGNGTNAGTGACNGTNTAGTG360.11005472165326649No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTNAGTTACCGTCCAGTA360.11005472165326649No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTNNAGTA360.11005472165326649No Hit
CNTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGTTATCGTCCAGTA360.11005472165326649No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTNCAGTA360.11005472165326649No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTACAGTCTAGTG360.11005472165326649No Hit
CCTGTTTGCTCCCCACGCTTTNGAGCCTCAGCGTCAGTTATCGTCCAGTA350.10699764605178687No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTNAGTTACAGTCCAGCA350.10699764605178687No Hit
CCTGTTNGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCNCAGTA350.10699764605178687No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGNGTCAGTTACAGTNNAGCA350.10699764605178687No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTNAGNTATCGTCCAGTA350.10699764605178687No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCNTGGCA340.10394057045030725No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA340.10394057045030725No Hit
CCTGTTTGCTCCCNACGCTTTCGAGCNTCAGNGTNAGTTATNGTNCAGTA330.10088349484882761No Hit
CCTGTTNGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA330.10088349484882761No Hit
CCTGTTNGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA330.10088349484882761No Hit
CCTGTTTGCNCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTNCAGTA330.10088349484882761No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTNAACGTCAGTTACCGTNCAGTA330.10088349484882761No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)