FastQCFastQC Report
Sat 13 May 2023
EGAF00002454998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002454998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31099155
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCGATGTATCTCGTA14639704.707426938127419Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG4802581.5442799008526116Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCA3104520.9982650654012947Illumina Paired End Adapter 2 (100% over 32bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCGAGATCGGAAGAGCGGTTCAGC478280.15379195994231998Illumina Paired End Adapter 2 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)