FastQCFastQC Report
Fri 1 Jul 2022
EGAF00002455893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002455893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17378428
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC9746035.608119445556295No Hit
TTTGTTCGTTCGGCTCGCGTGATGGAATTCTCGGGTGCCAAGGAACTCCA8738525.028371956312734RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA6721933.867973558943306No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC5454663.1387534016310337No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGC3681452.1184021938002675No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGC3567142.0526252432038157No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA2935791.689330013048361RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGC2775661.597187041313518No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC2543051.4633371902222687No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA2106921.2123766315342217No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA1974701.1362938005670018No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC1687190.9708530598970172No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1596310.9185583414103968RNA PCR Primer, Index 1 (100% over 28bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC1429930.8228189569275195No Hit
TTTGTTCGTTCGGCTCGCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCA1314050.7561385874487612RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA1285590.7397619623593112RNA PCR Primer, Index 1 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC1256680.7231263955520028No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1210400.6964956784353568RNA PCR Primer, Index 1 (100% over 30bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1209270.6958454470105121RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGC1190940.6852978877030765No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC1143390.6579363795160299No Hit
TCCCTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCCAA1028130.5916127741818765No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC1025000.5898116906776608No Hit
TTTGGCAATGGTAGAACTCACATGGAATTCTCGGGTGCCAAGGAACTCCA1004520.5780269653849013RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA994150.5720597973533624RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG968230.5571447544047138RNA PCR Primer, Index 1 (100% over 29bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG953290.548547889371812No Hit
TTTGGCAATGGTAGAACTCACACTTGGAATTCTCGGGTGCCAAGGAACTC880760.5068122387134211RNA PCR Primer, Index 1 (100% over 26bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC860120.4949354452543118No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCC840850.48384698546957183No Hit
TCCCTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCAAG781860.44990260338852284No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA777440.44735922029311276No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA748600.4307639333085823No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA670800.3859957874210487No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCATGGAATTCTCGGGTGCC625980.36020519232234355No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA591160.34016885762049365RNA PCR Primer, Index 1 (100% over 28bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA573440.3299723081972662No Hit
TTTGGCAATGGTAGAACTCACACTGGAATTCTCGGGTGCCAAGGAACTCC552860.3181300403005381RNA PCR Primer, Index 1 (100% over 27bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGAATTCTCGGGTGCC547510.3150515109882206No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA530270.3051311660640421No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA522500.30066010573568563RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA519190.29875544554432654No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGACTTGGAATTCTCGGGTGCC514930.2963041306152662No Hit
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA483990.2785004489473962RNA PCR Primer, Index 1 (100% over 28bp)
TTTGTTCGTTCGGCTCGCGTGAATGGAATTCTCGGGTGCCAAGGAACTCC464880.26750405732900584RNA PCR Primer, Index 1 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGCC456320.26257841042929775No Hit
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA412060.2371100539128165RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC405220.23317413980136753RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC403340.23209233884675876RNA PCR Primer, Index 1 (100% over 27bp)
TTTGGCAATGGTAGAACTCACTGGAATTCTCGGGTGCCAAGGAACTCCAG400340.2303660607277022RNA PCR Primer, Index 1 (100% over 29bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC395300.22746591348768713No Hit
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG394840.22720121750943184No Hit
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA379310.2182648511131156RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC365970.21058866774371077RNA PCR Primer, Index 1 (100% over 27bp)
GCATGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCCA358510.2062959894876568No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCATGGAATTCTCGGGTGCCA358010.20600827646781403No Hit
TGAGATGAAGCACTGTAGCTCATGGAATTCTCGGGTGCCAAGGAACTCCA356190.20496100107558635RNA PCR Primer, Index 1 (100% over 28bp)
TTTGTTCGTTCGGCTCGCGTGTTGGAATTCTCGGGTGCCAAGGAACTCCA343780.19781996392308904RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA342750.1972272751022129RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG339240.19520752970291674RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT337550.1942350596958482RNA PCR Primer, Index 1 (100% over 30bp)
GGTTCCATGGTGTAATGGTTAGCACTCTGGACTCTGGAATTCTCGGGTGC337340.19411422022751423No Hit
GCATGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAG334040.19221531429655203No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA331030.1904832819170986RNA PCR Primer, Index 1 (100% over 28bp)
TAACACTGTCTGGTAAAGATGTGGAATTCTCGGGTGCCAAGGAACTCCAG323970.18642077407691882RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA317070.1824503344030887No Hit
GGTAAAATGGCTGAGTGAAGCATTGGACTGTATGGAATTCTCGGGTGCCA314810.18114987155339943No Hit
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA310480.1786582768015611RNA PCR Primer, Index 1 (100% over 28bp)
TTTGATCGTTCGGCTCGCGTGATGGAATTCTCGGGTGCCAAGGAACTCCA308560.1775534588053649RNA PCR Primer, Index 1 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA294390.16939967182302104No Hit
TGGAAGACTAGTGATTTTGTTGTTGGAATTCTCGGGTGCCAAGGAACTCC292520.1683236251288091RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA274580.15800048197685085RNA PCR Primer, Index 1 (100% over 28bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG268720.1546284853842937RNA PCR Primer, Index 1 (100% over 29bp)
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGTC261410.1504221210341925No Hit
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG256970.14786722941798877RNA PCR Primer, Index 1 (100% over 29bp)
TTTGTTCGTTCGGCTCGCGTGATTGGAATTCTCGGGTGCCAAGGAACTCC250260.14400612069169894RNA PCR Primer, Index 1 (100% over 27bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC246050.14158357706462288RNA PCR Primer, Index 1 (100% over 27bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC237620.1367327355500739No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC236710.1362090978539601RNA PCR Primer, Index 1 (100% over 26bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCC233810.1345403623388721No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCGTGGAATTCTCGGGTGCC232640.13386711387244002No Hit
GGTAAAATGGCTGAGTGAAGCATTGGACTGCCATGGAATTCTCGGGTGCC227900.13113959444433065No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT219750.12644987222089363No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC218630.12580539505644583No Hit
TCCCTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAAGG217290.12503432416326724Illumina Small RNA Adapter 2 (100% over 21bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA214420.12338285142936979RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA214400.12337134290857608No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA212940.12253122089063521No Hit
TTTGTTCGTTCGGCTCGCGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG212440.12224350787079245RNA PCR Primer, Index 1 (100% over 29bp)
AGCAGCATTGTACAGGGCTATGTTGGAATTCTCGGGTGCCAAGGAACTCC210330.12102935892705599RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAAGTTGTATTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA208260.11983822702490697RNA PCR Primer, Index 1 (100% over 28bp)
AGCAGAGTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAG207650.1194872171406988No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTTGGAATTCTCGGGTG205390.11818675429100953No Hit
TGAGGTAGGAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA202980.11679997753536742RNA PCR Primer, Index 1 (100% over 28bp)
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA195360.11241523111296373RNA PCR Primer, Index 1 (100% over 28bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA194530.11193762750002474RNA PCR Primer, Index 1 (100% over 28bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA185570.10678181018444245RNA PCR Primer, Index 1 (100% over 22bp)
ATAGGCGATAGAAATTGAAACCTTGGAATTCTCGGGTGCCAAGGAACTCC182880.1052339141376884RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA176750.10170655251441614RNA PCR Primer, Index 1 (100% over 28bp)
TTTGTTCGTTCGGCTCGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC175400.10092972736084069RNA PCR Primer, Index 1 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)