FastQCFastQC Report
Fri 1 Jul 2022
EGAF00002456003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002456003
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24537082
Sequences flagged as poor quality0
Sequence length13-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGATTTTATGTGAATCCCTGCGTAGGCAGTGGAGCAGGTTATAATAAG1171870.4775914267230309No Hit
GGTAGATCTCATTTTCTTTGGTTTCAGGATGAACCTGGCTGACTATCAGC588810.2399674093276454No Hit
CTCATTTTCTTTGGTTTCAGGATGAACCTGGCTGACTATCAGCTCCATGC546860.22287083688272308No Hit
GCCCTCTAGAAGCCGTTTGGTTTGCTCCTCAATCTCTACAGCTTTGGATAGGATAGCCTCCGGGGCTTCTTGCAT459710.18735316611812278No Hit
GCTCATTCCAGGATCGCAATATGCTGACTATCAGGCTCAGAAAGTCTTTT430700.17553024438684273No Hit
CCCTGCGTAGGCAGTGGAGCAGGTTATAATAAGCAGAAAGGCGAGACTCTTCATCAGCCATCTGCAGGGATGGA409830.16702475053879676No Hit
CGTTTATCGAATTCGCTGAACATTTCTGAGGAGAGGTTATGGATGTAGTGGGACAGGACGACGGCGCGGTCAAAC376640.15349828475936952No Hit
CTCAGAAAGTCTTTTTGATTCATCTGTTGGGCTTGCTCCTTGTCTTCGGG370520.15100410065059894No Hit
GGCAGCTGTTGATGGCCTTGGTAATGAACCCCCGGCCATGGGTATACCGTTTATCGAATTCGCTGAACATTTCTG363580.148175728474967No Hit
CCTCAATCTCTACAGCTTTGGATAGGATAGCCTCCGGGGCTTCTTGCATACCACGTACTTCCGTGACCAGATGAT363470.14813089836843682No Hit
CTCAGAAATAGATGAAATGGATGTGGGCTTAGCAGTTGTTGTTGTGGATGATTCGGCACTTCAGGAGCTTGAGAT356820.14542071465547532No Hit
CCTGCGTAGGCAGTGGAGCAGGTTATAATAAGCAGAAAGGCGAGACTCTT348380.14198102284534078No Hit
GGATGATTCGGCACTTCAGGAGCTTGAGATAATTGTCGATTTTATGTGAA336660.13720457876776057No Hit
CTCGAAGGGTCACCTGGCATCGGGCAGCCCCGCCGGGACAGATGGGCAAGGGGGCCACGCTCTGGCACAGGAGC335930.1369070698789693No Hit
GGAGAGGTTATGGATGTAGTGGGACAGGACGACGGCGCGGTCAAACAGGTCTCGAAGGGTCACCTGGCATCGGGC324560.1322732670494397No Hit
GTGGATGATTCGGCACTTCAGGAGCTTGAGATAATTGTCGATTTTATGTG322890.13159266452302681No Hit
CTCTAGAAGCCGTTTGGTTTGCTCCTCAATCTCTACAGCTTTGGATAGGATAGCCTCCGGGGCTTCTTGCATACC322730.13152745709534655No Hit
GGGCTTAGCAGTTGTTGTTGTGGATGATTCGGCACTTCAGGAGCTTGAGA293900.11977789372020684No Hit
GTCAGTTTTTATTTTTTAAGAGGAGACCTGTTACACCCAAGCATGGATTC288940.11775646346211827No Hit
GCTTGAGATAATTGTCGATTTTATGTGAATCCCTGCGTAGGCAGTGGAGCAGGTTATAATAAGCAGAAAGGCG288270.11748340735870712No Hit
GTCCCGACCAGACAGGGTAGATCTCATTTTCTTTGGTTTCAGGATGAACC281690.11480175189535577No Hit
CCCGACCAGACAGGGTAGATCTCATTTTCTTTGGTTTCAGGATGAACCTGGCTGACTATCAGCTCCATGCCCTCT272930.11123164522986066No Hit
CTTTGGTTTCAGGATGAACCTGGCTGACTATCAGCTCCATGCCCTCTAGA271210.1105306653822977No Hit
GGACCTTCTCAGAAATAGATGAAATGGATGTGGGCTTAGCAGTTGTTGTT266630.10866410276494981No Hit
CGGCACTTCAGGAGCTTGAGATAATTGTCGATTTTATGTGAATCCCTGCGTAGGCAGTGGAGCAGGTTATAATA260800.10628810711884974No Hit
CTGGCATCGGGCAGCCCCGCCGGGACAGATGGGCAAGGGGGCCACGCTCT250470.10207815256924194No Hit
GACAGGACGACGGCGCGGTCAAACAGGTCTCGAAGGGTCACCTGGCATCGGGCAGCCCCGCCGGGACAGATGGGC250180.10195996410657143No Hit
GTAGATCTCATTTTCTTTGGTTTCAGGATGAACCTGGCTGACTATCAGCT249000.1014790593274294No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)