FastQCFastQC Report
Fri 1 Jul 2022
EGAF00002456079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002456079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26838444
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGTTCGTTCGGCTCGCGTGATGGAATTCTCGGGTGCCAAGGAACTCCA22790018.491554130336318RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC13170704.907400742010229No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC8053223.000628501413867No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA7702852.870080694693031No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA6025552.2451189793268194RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC4170901.5540766819417697No Hit
TTTGGCAATGGTAGAACTCACATGGAATTCTCGGGTGCCAAGGAACTCCA3245231.2091721859881297RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGC3196651.1910712856527748No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGC3082121.1483974257225942No Hit
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGTC2987681.1132090966227401No Hit
TTTGTTCGTTCGGCTCGCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCA2968251.1059694816882828RNA PCR Primer, Index 1 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGC2907811.0834495472241237No Hit
TTTGGCAATGGTAGAACTCACACTTGGAATTCTCGGGTGCCAAGGAACTC2821521.0512979068384143RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA2709951.0097269424412236RNA PCR Primer, Index 1 (100% over 28bp)
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT2547260.949108674109423No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGC2070020.7712891254053328No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC1920990.7157605709183439No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC1835670.6839703523795939No Hit
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCTTGATGAAAGCACA1806100.6729525750449616No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC1742520.649262677076212No Hit
TTTGGCAATGGTAGAACTCACACTGGAATTCTCGGGTGCCAAGGAACTCC1718610.6403538148485807RNA PCR Primer, Index 1 (100% over 27bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA1700970.6337811536317083No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1692160.6304985490216944RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC1626860.6061677793243155No Hit
CACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGT1588860.5920089853197152No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1538110.5730995433267294RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1498940.5585048075067243RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA1464830.5457954268883844No Hit
GCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGAA1458660.5434964858618481No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC1425640.5311932390715348No Hit
TTTGTTCGTTCGGCTCGCGTGAATGGAATTCTCGGGTGCCAAGGAACTCC1415200.527303296718692RNA PCR Primer, Index 1 (100% over 27bp)
TTTGGCAATGGTAGAACTCACTGGAATTCTCGGGTGCCAAGGAACTCCAG1397500.5207082795112861RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA1390610.5181410665983467RNA PCR Primer, Index 1 (100% over 28bp)
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT1343810.5007033939821549No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC1212100.4516282687625259No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA1157420.4312545093895905No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA1119860.41725965931556985No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA1084560.40410688488498064No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1083280.40362995708693095RNA PCR Primer, Index 1 (100% over 30bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC978150.36445853567367764No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA970400.36157088689642364RNA PCR Primer, Index 1 (100% over 28bp)
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATA951320.35446168190674543No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC911450.33960612619718195No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC882270.32873366280101785RNA PCR Primer, Index 1 (100% over 27bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCG881500.3284467609225036No Hit
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG861390.3209537780953322RNA PCR Primer, Index 1 (100% over 29bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGGG841790.31365082118769627No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCC825380.30753645777676236No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC787770.2935229777106303RNA PCR Primer, Index 1 (100% over 27bp)
TTTGTTCGTTCGGCTCGCGTGTTGGAATTCTCGGGTGCCAAGGAACTCCA784920.2924610681602853RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA784440.2922822202360167No Hit
TCAAGTGATGGAGAGCAATACCCGGGGATGACTGTGACCACATTGGGATG783980.29211082430859253No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA782310.2914885825720746RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA762750.2842005296581277RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT753950.2809216510465361RNA PCR Primer, Index 1 (100% over 30bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC750380.27959146960978815No Hit
TAACACTGTCTGGTAAAGATGTGGAATTCTCGGGTGCCAAGGAACTCCAG725270.27023548757148513RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC670000.24964189429163627RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA627940.23397034492759713RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA625760.23315807727154375RNA PCR Primer, Index 1 (100% over 28bp)
TTTGATCGTTCGGCTCGCGTGATGGAATTCTCGGGTGCCAAGGAACTCCA625330.23299785933938644RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA623250.23222285166755569No Hit
TTTGGCAATGGTAGAACTCATGGAATTCTCGGGTGCCAAGGAACTCCAGT619730.230911300222919RNA PCR Primer, Index 1 (100% over 30bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCATGGAATTCTCGGGTGCC611620.22788951550246356No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA603480.2248565527867413No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGG599320.22330653744307977No Hit
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG584480.21777715578444115RNA PCR Primer, Index 1 (100% over 29bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGAATTCTCGGGTGCC575030.2142560872754024No Hit
CTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACACA563900.21010905103142344No Hit
TCCCTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCCAA557530.20773559003644174No Hit
TTTGTTCGTTCGGCTCGCGTGATTGGAATTCTCGGGTGCCAAGGAACTCC553150.20610360272749045RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA521640.19436298169893904RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA512000.19077111921987727RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCC498680.18580808932142265RNA PCR Primer, Index 1 (100% over 27bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG497540.18538332550128467No Hit
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGACTGCTGACGCGGG492980.1836842702207326No Hit
AACATTCAACCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG488620.18205973490862587RNA PCR Primer, Index 1 (100% over 29bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA487850.1817728330301116RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC463400.17266276688767798RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG460160.1714555433988647RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCAAG439540.16377253465215794No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCTGATGCCATCACCG435150.16213682134478438No Hit
TTGGAGGATGAAAGTACGGAGTGATCCATCGGCTAAGTGTCTTGTCACAA432750.1612425817234412No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG425700.15861575283574564No Hit
GGCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGA423010.15761345926015682No Hit
TTTGTTCGTTCGGCTCGCGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG421240.1569539575394162RNA PCR Primer, Index 1 (100% over 29bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA413620.15411474674165163No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG411730.1534105330398439No Hit
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG408130.15206917360782912RNA PCR Primer, Index 1 (100% over 29bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA407710.15191268167409408RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG405130.15095137408115017RNA PCR Primer, Index 1 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC395180.14724400565099824No Hit
TTTGTTCGTTCGGCTCGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC394540.1470055417519734RNA PCR Primer, Index 1 (100% over 31bp)
CGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTCTGA389420.14509783055977463No Hit
TGAGGTAGTAAGTTGTATTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA388520.14476249070177094RNA PCR Primer, Index 1 (100% over 28bp)
TATGGCACTGGTAGAATTCACTTGGAATTCTCGGGTGCCAAGGAACTCCA377720.14073841240572665RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT374050.13937097098475604RNA PCR Primer, Index 1 (100% over 30bp)
AGCAGCATTGTACAGGGCTATGTTGGAATTCTCGGGTGCCAAGGAACTCC373370.13911760309204213RNA PCR Primer, Index 1 (100% over 27bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC363870.13557790459089208RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC361160.13456815901845876RNA PCR Primer, Index 1 (100% over 26bp)
GACCAATGATGACAAATACCGGCGTATGAGTCTTGGATGATGAATAATAC357780.1333087715517338No Hit
CTGCGATGATGGCATTTCTTAGGACACCTTTGGATTAATAATGAAAACAA349080.13006715292436477No Hit
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG341140.12710871017708777RNA PCR Primer, Index 1 (100% over 29bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA338260.12603562263147594No Hit
TGGAAGACTAGTGATTTTGTTGTTGGAATTCTCGGGTGCCAAGGAACTCC335090.12485448113161851RNA PCR Primer, Index 1 (100% over 27bp)
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA331850.12364725764280522No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTTGGAATTCTCGGGTG326420.1216240404995163No Hit
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA310910.11584501694658603RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTATGATGAATTTGATTGCATTGATCGTCTGACATGATAATGTATTT309800.1154314311217148No Hit
AGTGATGATGACCCCAGGTAACTCTTGAGTGTGTCGCTGATGCCATCACC301170.11221589448330163No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA300930.11212647052116732RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA295880.11024484131792439RNA PCR Primer, Index 1 (100% over 28bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA295430.11007717138892255RNA PCR Primer, Index 1 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCATGGAATTCTCGGGTGCCA294710.1098088995025196No Hit
TTTTGTTCGTTCGGCTCGCGTGATGGAATTCTCGGGTGCCAAGGAACTCC293990.10954062761611663RNA PCR Primer, Index 1 (100% over 27bp)
TTTGAACGTTCGGCTCGCGTGATGGAATTCTCGGGTGCCAAGGAACTCCA292070.1088252359190421RNA PCR Primer, Index 1 (100% over 28bp)
TTGTTCGTTCGGCTCGCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG288130.10735719254067039RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGGAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA269890.10056097141846226RNA PCR Primer, Index 1 (100% over 28bp)
CAGTGCAATAGTATTGTCAAAGCTGGAATTCTCGGGTGCCAAGGAACTCC268600.1000803176219903RNA PCR Primer, Index 1 (100% over 27bp)
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAATC268390.10000207165512277No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)