FastQCFastQC Report
Tue 5 Jul 2022
EGAF00002456118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002456118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47745217
Sequences flagged as poor quality0
Sequence length8-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGAACTTGCTGTAGGTCTGCTTGAAGATCTGCCCAGTCCGGGGGCTGC1510700.3164086572273826No Hit
GTCGGAATAGACTCTGAGAAACAGAGGGAGGTCTGGGGGTTCTGCAGGAA1359060.2846484078185256No Hit
CGTAGTTCTTGAGTAGTGCGTCATCGTTGTGTGAGTTTGTGTCGAACTTG1129600.23658914357850758No Hit
GTCAAAGGCCAGCTGGTGCAGACGATGGGCGCGGAGCATAGCGTTGTCAA1049510.21981468845350519No Hit
GTTGGATTTCTGTTGTGTTTCCTCCCTGTTGGAGGGTGTCGGAATAGACT964800.2020725971357508No Hit
CCTGGATAAGGGAATGGTTGGGAAGGCACTGCCCTCTTGAAGCCAGGGCA916570.1919710617295969No Hit
GTGAGTTTGTGTCGAACTTGCTGTAGGTCTGCTTGAAGATCTGCCCAGTC915930.19183701688904253No Hit
CTCGACCTTGTCCATGTCCTTCCTGAAGCAGTAGAGCAGCCCGTAGTTCTTGAGTAGTGCGTCATCGTTGTGTG874420.18314295230870978No Hit
GTCAGAGGCGCCGTACACCAGGCTGTTGGCGAAGACACTCCTGAGGAACT835580.17500810604756495No Hit
GTGCAGACGATGGGCGCGGAGCATAGCGTTGTCAAAAAGCCTGGATAAGG828090.17343936252295178No Hit
GTCACAGGGATGCCACCCGGGCAGCTAGAAGCCACAGCTGCCCTCCACAG811160.1698934576001613No Hit
GGCTTCTTCAAACTCCTGGTAGGTGTCAAAGGCCAGCTGGTGCAGACGATGGGCGCGGAGCATAGCGTTGTCAAA791580.16579252325945026No Hit
CTGGTGGGCACTGGAGTGGCAACTTCCAGGGCCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGC718320.15044857791723934No Hit
CCGTAGTTCTTGAGTAGTGCGTCATCGTTGTGTGAGTTTGTGTCGAACTT688220.14414428150991546No Hit
GTAGTTCTTGAGTAGTGCGTCATCGTTGTGTGAGTTTGTGTCGAACTTGCTGTAGGTCTGCTTGAAGATCTGCCC683320.14311800069942085No Hit
GTTGTGTTTCCTCCCTGTTGGAGGGTGTCGGAATAGACTCTGAGAAACAGAGGGAGGTCTGGGGGTTCTGCAGGA674610.14129373419750088No Hit
CACAGGGATGCCACCCGGGCAGCTAGAAGCCACAGCTGCCCTCCACAGAG655830.13736035590748286No Hit
GCTTCTTCAAACTCCTGGTAGGTGTCAAAGGCCAGCTGGTGCAGACGATGGGCGCGGAGCATAGCGTTGTCAAAA650050.13614976344122595No Hit
GTCCTTTAGGAGGTCATAGACGTTGCTGTCAGAGGCGCCGTACACCAGGC631670.13230016317655444No Hit
CCTTCCTCTAGGTCCTTTAGGAGGTCATAGACGTTGCTGTCAGAGGCGCC608420.1274305654532893No Hit
GTTGGGAAGGCACTGCCCTCTTGAAGCCAGGGCAGGCAGAGCAGGCCAAA601860.12605660583760672No Hit
CTTTGGGATATAGGCTTCTTCAAACTCCTGGTAGGTGTCAAAGGCCAGCTGGTGCAGACGATGGGCGCGGAGCAT584200.12235780601855889No Hit
GTCATCGTTGTGTGAGTTTGTGTCGAACTTGCTGTAGGTCTGCTTGAAGA570060.11939625282256022No Hit
GCCCGTAGTTCTTGAGTAGTGCGTCATCGTTGTGTGAGTTTGTGTCGAAC552010.11561576942879954No Hit
GTTCCTTTGGGATATAGGCTTCTTCAAACTCCTGGTAGGTGTCAAAGGCCAGCTGGTGCAGACGATGGGCGCGG551130.11543145777303725No Hit
GTCCTTCCTGAAGCAGTAGAGCAGCCCGTAGTTCTTGAGTAGTGCGTCAT540710.113249040212761No Hit
GCAACTTAATTTTATTAGGACAAGGCTGGTGGGCACTGGAGTGGCAACTTCCAGGGCCAGGAGAGGCACTGGGG538910.11287203909870176No Hit
GTCCATGTCCTTCCTGAAGCAGTAGAGCAGCCCGTAGTTCTTGAGTAGTG534230.11189183620214775No Hit
GTCTCGACCTTGTCCATGTCCTTCCTGAAGCAGTAGAGCAGCCCGTAGTT530670.11114621177656392No Hit
GTCTGCTTGAAGATCTGCCCAGTCCGGGGGCTGCCATCTTCCAGCCTCCCCATCAGCGTTTGGATGCCTTCCTCT523980.10974502430264375No Hit
CTCCTGGTAGGTGTCAAAGGCCAGCTGGTGCAGACGATGGGCGCGGAGCATAGCGTTGTCAAAAAGCCTGGATA514440.1077469183981298No Hit
CCTTCCTGAAGCAGTAGAGCAGCCCGTAGTTCTTGAGTAGTGCGTCATCG509530.10671854313700155No Hit
GTCAAAAAGCCTGGATAAGGGAATGGTTGGGAAGGCACTGCCCTCTTGAAGCCAGGGCAGGCAGAGCAGGCCAAA507830.10636248652927895No Hit
GGCAACTTCCAGGGCCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCC505790.1059352186000118No Hit
GGGGTCACAGGGATGCCACCCGGGCAGCTAGAAGCCACAGCTGCCCTCCA504630.10569226232650696No Hit
GGCGAAGACACTCCTGAGGAACTGCACGGGCTCCAGCCACGACTGGATGA478710.10026344628405397No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)