FastQCFastQC Report
Fri 1 Jul 2022
EGAF00002456136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002456136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40630839
Sequences flagged as poor quality0
Sequence length5-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGATTTTATGTGAATCCCTGCGTAGGCAGTGGAGCAGGTTATAATAAG2455630.6043759027471719No Hit
GGTAGATCTCATTTTCTTTGGTTTCAGGATGAACCTGGCTGACTATCAGC1468850.36151111720828605No Hit
GTCAGTTTTTATTTTTTAAGAGGAGACCTGTTACACCCAAGCATGGATTC1345510.33115486490446333No Hit
CTCATTTTCTTTGGTTTCAGGATGAACCTGGCTGACTATCAGCTCCATGC1252680.3083076871732824No Hit
GCTCATTCCAGGATCGCAATATGCTGACTATCAGGCTCAGAAAGTCTTTT1113610.2740799913090645No Hit
CTCAGAAATAGATGAAATGGATGTGGGCTTAGCAGTTGTTGTTGTGGATGATTCGGCACTTCAGGAGCTTGAGAT1103140.2715031309099967No Hit
CGTTTATCGAATTCGCTGAACATTTCTGAGGAGAGGTTATGGATGTAGTGGGACAGGACGACGGCGCGGTCAAAC1098880.27045466622040465No Hit
GCCCTCTAGAAGCCGTTTGGTTTGCTCCTCAATCTCTACAGCTTTGGATAGGATAGCCTCCGGGGCTTCTTGCAT1086020.2672895826738897No Hit
GGGCTTAGCAGTTGTTGTTGTGGATGATTCGGCACTTCAGGAGCTTGAGA1030190.25354878839691203No Hit
CTCAGAAAGTCTTTTTGATTCATCTGTTGGGCTTGCTCCTTGTCTTCGGG1001530.24649503299698045No Hit
GGCAGCTGTTGATGGCCTTGGTAATGAACCCCCGGCCATGGGTATACCGTTTATCGAATTCGCTGAACATTTCTG977550.24059311204476974No Hit
GGATGATTCGGCACTTCAGGAGCTTGAGATAATTGTCGATTTTATGTGAA870380.21421659542890564No Hit
CCCTGCGTAGGCAGTGGAGCAGGTTATAATAAGCAGAAAGGCGAGACTCTTCATCAGCCATCTGCAGGGATGGA788030.1939487392815098No Hit
CCTGCGTAGGCAGTGGAGCAGGTTATAATAAGCAGAAAGGCGAGACTCTT784900.19317838846497853No Hit
CTCTAGAAGCCGTTTGGTTTGCTCCTCAATCTCTACAGCTTTGGATAGGATAGCCTCCGGGGCTTCTTGCATACC767290.1888442421777212No Hit
CCTCAATCTCTACAGCTTTGGATAGGATAGCCTCCGGGGCTTCTTGCATACCACGTACTTCCGTGACCAGATGAT756270.18613201661919904No Hit
CGGCACTTCAGGAGCTTGAGATAATTGTCGATTTTATGTGAATCCCTGCGTAGGCAGTGGAGCAGGTTATAATA746700.18377666284469293No Hit
GCTTGAGATAATTGTCGATTTTATGTGAATCCCTGCGTAGGCAGTGGAGCAGGTTATAATAAGCAGAAAGGCG742670.18278480540359995No Hit
GCAGGTTATAATAAGCAGAAAGGCGAGACTCTTCATCAGCCATCTGCAGGGATGGAAGTCCCGACCAGACAGGGT722470.1778132122745484No Hit
GTGGGCTTAGCAGTTGTTGTTGTGGATGATTCGGCACTTCAGGAGCTTGA714100.17575320066612457No Hit
GTAGATCTCATTTTCTTTGGTTTCAGGATGAACCTGGCTGACTATCAGCT709830.17470227479181513No Hit
GGACCTTCTCAGAAATAGATGAAATGGATGTGGGCTTAGCAGTTGTTGTT692460.17042719693777428No Hit
GGAGAGGTTATGGATGTAGTGGGACAGGACGACGGCGCGGTCAAACAGGTCTCGAAGGGTCACCTGGCATCGGGC687430.1691892210249461No Hit
CCCGACCAGACAGGGTAGATCTCATTTTCTTTGGTTTCAGGATGAACCTGGCTGACTATCAGCTCCATGCCCTCT682610.16800292999118233No Hit
GTGGATGATTCGGCACTTCAGGAGCTTGAGATAATTGTCGATTTTATGTG677560.16676003170891943No Hit
GCTTGCAATGGAACGGATCATTAAGGACCTTCTCAGAAATAGATGAAATG638970.15726231988465708No Hit
CGCTGAACATTTCTGAGGAGAGGTTATGGATGTAGTGGGACAGGACGACG584140.14376764407941467No Hit
CACGTACTTCCGTGACCAGATGATACAGAGGCTCATTCCAGGATCGCAAT572000.14077976583254903No Hit
CTTCTCAGAAATAGATGAAATGGATGTGGGCTTAGCAGTTGTTGTTGTGGATGATTCGGCACTTCAGGAGCTTG559020.1375851480694258No Hit
CTTAGCAGTTGTTGTTGTGGATGATTCGGCACTTCAGGAGCTTGAGATAA555690.1367655735585475No Hit
GGCACTTCAGGAGCTTGAGATAATTGTCGATTTTATGTGAATCCCTGCGT553960.13633978860244556No Hit
CTCTAAGGAGTCAGTTTTTATTTTTTAAGAGGAGACCTGTTACACCCAAGCATGGATTCAAAAGAGATACAACTA552210.1359090812769089No Hit
CTCGAAGGGTCACCTGGCATCGGGCAGCCCCGCCGGGACAGATGGGCAAGGGGGCCACGCTCTGGCACAGGAGC535410.1317742909517571No Hit
CTTTGGTTTCAGGATGAACCTGGCTGACTATCAGCTCCATGCCCTCTAGA524960.129202352922124No Hit
CCAGGATCGCAATATGCTGACTATCAGGCTCAGAAAGTCTTTTTGATTCA511970.12600527397428343No Hit
GGAACGGATCATTAAGGACCTTCTCAGAAATAGATGAAATGGATGTGGGC500990.12330289315463064No Hit
GTCCCGACCAGACAGGGTAGATCTCATTTTCTTTGGTTTCAGGATGAACC500670.12322413524367538No Hit
GCTGAACATTTCTGAGGAGAGGTTATGGATGTAGTGGGACAGGACGACGG497690.12249070219790441No Hit
CCGGGGCTTCTTGCATACCACGTACTTCCGTGACCAGATGATACAGAGGC494630.12173757967439461No Hit
GCTGACTATCAGGCTCAGAAAGTCTTTTTGATTCATCTGTTGGGCTTGCTCCTTGTCTTCGGGGGTGGCAAGGGA493160.12137578552094384No Hit
GGCTTAGCAGTTGTTGTTGTGGATGATTCGGCACTTCAGGAGCTTGAGAT491830.12104844795353598No Hit
GGCAAGGGAAGAAGTGTGGCAGCTGTTGATGGCCTTGGTAATGAACCCCC482770.11881861459961485No Hit
CTCCGGGGCTTCTTGCATACCACGTACTTCCGTGACCAGATGATACAGAG457750.1126607304367995No Hit
GCCGTTTGGTTTGCTCCTCAATCTCTACAGCTTTGGATAGGATAGCCTCC450580.11089606099445792No Hit
GACAGGACGACGGCGCGGTCAAACAGGTCTCGAAGGGTCACCTGGCATCGGGCAGCCCCGCCGGGACAGATGGGC448730.11044074182174775No Hit
CTAGAAGCCGTTTGGTTTGCTCCTCAATCTCTACAGCTTTGGATAGGATA447800.11021185164303399No Hit
GGCTCATTCCAGGATCGCAATATGCTGACTATCAGGCTCAGAAAGTCTTT444420.10937997120856893No Hit
CTTGCAATGGAACGGATCATTAAGGACCTTCTCAGAAATAGATGAAATGG443880.10924706723383193No Hit
GTTGGGCTTGCTCCTTGTCTTCGGGGGTGGCAAGGGAAGAAGTGTGGCAG434450.10692617004536874No Hit
CTTGTCTTCGGGGGTGGCAAGGGAAGAAGTGTGGCAGCTGTTGATGGCCTTGGTAATGAACCCCCGGCCATGGGT420080.10338944760653354No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)