FastQCFastQC Report
Fri 1 Jul 2022
EGAF00002456137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002456137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40630839
Sequences flagged as poor quality0
Sequence length2-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAAACCAAAGAAAATGAGATCTACCCTGTCTGGTCGGGACTTCCATCC1276590.31419237983247156No Hit
CCTGAAACCAAAGAAAATGAGATCTACCCTGTCTGGTCGGGACTTCCATC1178060.28994232681239984No Hit
GTGTGTTTCCTGCAACGATCACGAACATGAACATCAAAGGATCGCCATGG1134990.2793420042347637No Hit
CTCTGGTGAAGTGTGTTTCCTGCAACGATCACGAACATGAACATCAAAGG1054110.2594359422408186No Hit
CTTCTCTGGTGAAGTGTGTTTCCTGCAACGATCACGAACATGAACATCAA929140.22867851682806745No Hit
CGAACATGAACATCAAAGGATCGCCATGGAAAGGGTCCCTCCTGCTGCTGCTGGTGTCAAACCTGCTCCTGTGCC902090.2220210121676296No Hit
CGGAAGTACGTGGTATGCAAGAAGCCCCGGAGGCTATCCTATCCAAAGCT871560.21450701522555318No Hit
ATGAGATCTACCCTGTCTGGTCGGGACTTCCATCCCTGCAGATGGCTGATGAAGAGTCTCGCCTTTCTGCTTATT842800.20742864797844812No Hit
CCGAAGACAAGGAGCAAGCCCAACAGATGAATCAAAAAGACTTTCTGAGC769000.18926510476438846No Hit
CAGGGATTCACATAAAATCGACAATTATCTCAAGCTCCTGAAGTGCCGAA717310.17654324096039464No Hit
CTTCCATCCCTGCAGATGGCTGATGAAGAGTCTCGCCTTTCTGCTTATTA706990.1740032983320871No Hit
GTTTCCTGCAACGATCACGAACATGAACATCAAAGGATCGCCATGGAAAGGGTCCCTCCTGCTGCTGCTGGTGTC698840.17199743278744503No Hit
CCCGAAGACAAGGAGCAAGCCCAACAGATGAATCAAAAAGACTTTCTGAG676210.16642777177207688No Hit
CCTATCCAAAGCTGTAGAGATTGAGGAGCAAACCAAACGGCTTCTAGAGG648130.15951676508575174No Hit
CTTCGAGACCTGTTTGACCGCGCCGTCGTCCTGTCCCACTACATCCATAA625980.1540652409368165No Hit
CACTTCTTCCCTTGCCACCCCCGAAGACAAGGAGCAAGCCCAACAGATGAATCAAAAAGACTTTCTGAGCCTGAT617020.15186001943006888No Hit
GTCGGGACTTCCATCCCTGCAGATGGCTGATGAAGAGTCTCGCCTTTCTGCTTATTATAACCTGCTCCACTGCCT541110.13317716624064788No Hit
CAAGGAGCAAGCCCAACAGATGAATCAAAAAGACTTTCTGAGCCTGATAGTCAGCATATTGCGATCCTGGAATG525780.1294041700689469No Hit
GATGAATCAAAAAGACTTTCTGAGCCTGATAGTCAGCATATTGCGATCCT506120.12456548091463236No Hit
GGCTGATGAAGAGTCTCGCCTTTCTGCTTATTATAACCTGCTCCACTGCC504900.12426521637911538No Hit
CGCAGGGATTCACATAAAATCGACAATTATCTCAAGCTCCTGAAGTGCCGAATCATCCACAACAACAACTGCTA472130.11619991406035203No Hit
ATCCTATCCAAAGCTGTAGAGATTGAGGAGCAAACCAAACGGCTTCTAGA468580.11532619348569198No Hit
CAGCATATTGCGATCCTGGAATGAGCCTCTGTATCATCTGGTCACGGAAGTACGTGGTATGCAAGAAGCCCCGG448830.11046535366892127No Hit
CGAAGACAAGGAGCAAGCCCAACAGATGAATCAAAAAGACTTTCTGAGCC448820.11046289248420393No Hit
GTTTGACCGCGCCGTCGTCCTGTCCCACTACATCCATAACCTCTCCTCAG443670.10919538235476753No Hit
GCGAATTCGATAAACGGTATACCCATGGCCGGGGGTTCATTACCAAGGCC442670.10894926388303229No Hit
CTCTCCTCAGAAATGTTCAGCGAATTCGATAAACGGTATACCCATGGCCGGGGGTTCATTACCAAGGCCATCAAC431060.10609182842618634No Hit
GTCAGCATATTGCGATCCTGGAATGAGCCTCTGTATCATCTGGTCACGGA413560.10178475517081988No Hit
GTTCATTACCAAGGCCATCAACAGCTGCCACACTTCTTCCCTTGCCACCCCCGAAGACAAGGAGCAAGCCCAAC413280.10171584199873403No Hit
CTCTGTATCATCTGGTCACGGAAGTACGTGGTATGCAAGAAGCCCCGGAGGCTATCCTATCCAAAGCTGTAG411790.10134912547584854No Hit
ATAAAATCGACAATTATCTCAAGCTCCTGAAGTGCCGAATCATCCACAAC410210.10096025829050687No Hit
GTCCTGTCCCACTACATCCATAACCTCTCCTCAGAAATGTTCAGCGAATT407200.10021944169058385No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)