FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002458263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002458263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145609
Sequences flagged as poor quality0
Sequence length35-250
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTCTCAACGACAGGTTGCTACAAAGAGAAGTCTTATCGGTCTTAATTC6116542.0063320261797No Hit
CGTGTGGAGGGGTTTTTAAGCCCCCAGCAAAGTGACCCTGATGCCATGAA5845840.147243645653774No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10830.7437727063574368No Hit
GGTCTCAACGACAGGTTGCTACAAAGAGAAGTCTTATCGGTCTTAATTCA6550.44983483163815424No Hit
AGGTCTCAACGACAGGTTGCTACAAAGAGAAGTCTTATCATTGGCCAGGA5190.35643401163389626No Hit
AGGTCTCAACGACAGGTTGCTACAAAGAGAAGCCTTATCGGTCTTAATTC3360.23075496706934326No Hit
GTGTGGAGGGGTTTTTAAGCCCCCAGCAAAGTGACCCTGATGCCATGAAC2950.20259736692100078No Hit
CGTGTGGAGGGGTTTTTAAGCCCCCAGCAAAGCGACCCTGATGCCATGAA2410.16551174721342773No Hit
CGTGTGGAGGGGGTTTTAAGCCCCCAGCAAAGTGACCCTGATGCCATGAA2350.1613911228014752No Hit
CGTGTGGAGGGGCTTTTAAGCCCCCAGCAAAGTGACCCTGATGCCATGAA2200.15108956177159377No Hit
GTGGAGGGGTTTTTAAGCCCCCAGCAAAGTGACCCTGATGCCATGAACCT1950.13392029338845812No Hit
CGTGTGGAGGGGTTTTTAAGCCCCCAGCAAAGTGACCCCCAGTTCTGCCA1920.13185998118248185No Hit
AGGTCTCAACGACAGGTTGCTACAAAGAGAAGTCTTATCGGCCTTAATTC1850.12705258603520386No Hit
GTCTCAACGACAGGTTGCTACAAAGAGAAGTCTTATCGGTCTTAATTCAT1820.12499227382922759No Hit
CGTGTGGAGGGGTTTTTAAGCCCCCAGCAAAGTGACCCTTTTCTGTGAAT1800.12361873235857673No Hit
AGGTCTCAACGACAGGTTGCTACAAAGAGAGGTCTTATCGGTCTTAATTC1690.11606425426999704No Hit
AGGTCTCAACGACAGGTTGCTACAAAGAGAAGTCTCTAGATCTATCCCTT1530.10507592250479023No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)