FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002458264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002458264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151671
Sequences flagged as poor quality0
Sequence length35-250
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCACGTGGCTGTCACTGCGGCTCCCACTCAGGCCACCGTCTTCTCCTC6735744.409939935782056No Hit
ACTCAACCTCGAAAGCTCCAGAGCCCCCAATGGATGCCTGAATCCAGCCT5193534.24187880346276No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN75424.972605178313587No Hit
GCACGTGGCTGTCACTGCGGCTCCCACTCAGGCCACCGTCTTCTCCTCCA33192.1882891258051966No Hit
CTGTACGTGGCTGTCACTGCGGCTCCCACTCAGGCCACCGTCTTCTCCTC25291.6674248867614772No Hit
ACTTAACCTCGAAAGCTCCAGAGCCCCCAATGGATGCCTGAATCCAGCCT20671.3628182051941373No Hit
CTGCACGTGGCTGTCACTGCGGCTCCCACTCAGGCCACCGTCGCTGGGCA9950.6560252124664571No Hit
CTGCACGTGGCTGTCACTGCGGCTCCAACCAAAACCGTGAATGGTGTCTG8550.563720157446051No Hit
CAACCTCGAAAGCTCCAGAGCCCCCAATGGATGCCTGAATCCAGCCTCCC6630.4371303677037799No Hit
CTCAACCTCGAAAGCTCCAGAGCCCCCAATGGATGCCTGAATCCAGCCTC5200.3428473472186509No Hit
CTGCACGTGGCTGTCACTGGTACAGTTATCTGGAGCTTTCGAGGTTGAGT4520.29801346335159656No Hit
ACTCAACCTCGAAAGCTCCAGATAACTGTACCAGTGACAGCCACGTGCAG4000.26372872862973146No Hit
TGCACGTGGCTGTCACTGCGGCTCCCACTCAGGCCACCGTCTTCTCCTCC3400.22416941933527174No Hit
CTGCACGTGGCTGTCACTGCGGCTCCCACTCAGGCCACCGTCTTCTCCAA3320.21889484476267712No Hit
CTGCACGTGGCTGTCACTGCGGCTCCCACTCAGTGGACCATCGCTCCAAC2940.19384061554285265No Hit
ACTCAACCTCGAAAGCTCCAGAGCCCCCCCCGTGGGGGGTGCTGGGCACG2690.1773575700034944No Hit
CGTCCTCTAAGTGGGGAGGCTGGATTCAGGCATCCATTGGGGGCTCTGGAGCTTTCGAGGTTGAGT1560.10285420416559526No Hit
CTGCACGTGGCTGTCACTGCGGCCCCCACTCAGGCCACCGTCTTCTCCTC1520.10021691687929796No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)