FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002458265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002458265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151671
Sequences flagged as poor quality0
Sequence length35-250
%GC66

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCAACCTCGAAAGCTCCAGAGCCCCCAATGGATGCCTGAATCCAGCCT6322241.68364420357221No Hit
CTGCACGTGGCTGTCACTGCGGCTCCCACTCAGGCCACCGTCTTCTCCTC4861532.05293035583598No Hit
GCACGTGGCTGTCACTGCGGCTCCCACTCAGGCCACCGTCTTCTCCTCCA15931.0502996617679057No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9050.5966862485247675No Hit
CTGCACGTGGCTGTCACTGCGGCTCCCACTCAGGCCACCGTCGCTGGGCA8730.5755879502343889No Hit
CTGCACGTGGCTGTCACTGCGGCTCCAACCAAAACCGTGAATGGTGTCTG7630.5030625498612128No Hit
CTCAACCTCGAAAGCTCCAGAGCCCCCAATGGATGCCTGAATCCAGCCTC6920.4562507005294354No Hit
ACTCAACCTCGAAAGCTCCAGATAACTGTACCAGTGACAGCCACGTGCAG4680.30856261249678585No Hit
CTGCACGTGGCTGTCACTGGTACAGTTATCTGGAGCTTTCGAGGTTGAGT4090.2696626250239004No Hit
ACTCAACCTCGAAAGCTCCAGAGCCCCCAATGGCTGCCTGAATCCAGCCT4050.2670253377376031No Hit
ACTCAACCTCGCAAGCTCCAGAGCCCCCAATGGATGCCTGAATCCAGCCT3480.22944399390786638No Hit
ACTCAACCTCGAAAGCTCCAGAGCCCCCAATGGATGCCTGAATCCACCCT3300.21757620111952847No Hit
CTGCACGTGGCTGTCACTGCGGCCCCCACTCAGGCCACCGTCTTCTCCTC3180.2096643392606365No Hit
ACTCAACCTCGAAAGCTCCAGAGCCCCCCCCGTGGGGGGTGCTGGGCACG3050.20109315558017024No Hit
CTGCACGTGGCTGTCACTGCGGCTCCCACTCAGGCCACCGTCTTCTCCAA2990.19713722465072425No Hit
CTGCACGTGGCTGTCACTGCGGCTCCCACTCAGTGGACCATCGCTCCAAC2460.16219316810728485No Hit
TGCACGTGGCTGTCACTGCGGCTCCCACTCAGGCCACCGTCTTCTCCTCC2330.15362198442681857No Hit
ACTCAACCTCGACAGCTCCAGAGCCCCCAATGGATGCCTGAATCCAGCCT1950.1285677552069941No Hit
CAACCTCGAAAGCTCCAGAGCCCCCAATGGATGCCTGAATCCAGCCTCCC1780.1173592842402305No Hit
ACTCAACCTCGAAACCTCCAGAGCCCCCAATGGATGCCTGAATCCAGCCT1720.11340335331078452No Hit
ACTCAACCTCGAAAGCTCCAGAGCCCCCAATGGACGCCTGAATCCAGCCT1690.11142538784606154No Hit
ACTCAACCTCGAAAGCTCCAGAGCCCCCAATGGATGCCTGAATCCAGCCTCCCCACTTAGAGGACG1550.10219488234402094No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)