FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002458414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002458414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182857
Sequences flagged as poor quality0
Sequence length35-301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC91505.003910159304812No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC74074.050706289614289No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC56333.0805492816791262No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC55673.044455503480862No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT49302.696095856324888No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN48042.627189552491838No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT46292.531486352723713No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT45072.4647675505996487No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT43352.3707049771132636No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT42972.349923710877899No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT40292.2033610963758563No Hit
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC36882.016876575684825No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT33901.8539076983653893No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT32231.762579502015236No Hit
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC31541.7248450975352325No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT31411.7177357169810288No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT30761.682188814210011No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT29321.6034387526865257No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT29111.5919543687143507No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC24671.3491416790169366No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC24541.342032298462733No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC21441.1725009160163407No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC16450.8996100778203733No Hit
CCTACGGGGGGCTGCAGTCGGGAATTTTGCTCAATGGGCGAAAGCCTGAA14180.7754693558354343No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT13610.7442974564823879No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC11960.6540630109867274No Hit
CCTACGGGTGGCTGCAGTCGGGAATTTTGCTCAATGGGCGAAAGCCTGAA11740.642031751587306No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT11530.630547367615131No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC11470.6272661150516523No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC11280.6168754819339702No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT11080.6059379733890418No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC11060.6048442225345488No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT10910.5966410911258524No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT9700.5304691644290347No Hit
CCTACGGGAGGCTGCAGTCGGGAATTTTGCTCAATGGGCGAAAGCCTGAA9080.4965628879397562No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC8770.47960974969511694No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT8640.4725003691409134No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC8530.4664847394412027No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT8460.46265661145047765No Hit
CCTACGGGCGGCTGCAGTCGGGAATTTTGCTCAATGGGCGAAAGCCTGAA8350.45664098175076695No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT7800.42656283325221345No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT7260.3970315601809064No Hit
CCTACGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC7140.39046905505394924No Hit
CCTACGGGGTGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC7130.38992217962670284No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT7110.38882842877220997No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT6800.37187529052757073No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC6480.35437527685568504No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC6450.35273465057394576No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT6330.3461721454469886No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT5970.3264846300661172No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5860.3204690003664065No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC5830.3188283740846673No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT5780.3160939969484351No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT5450.298047107849303No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5390.29476585528582444No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT5360.29312522900408516No Hit
CCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC5250.28710959930437446No Hit
CCTACGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC5030.27507833990495306No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT4980.2723439627687209No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC4890.2674220839235031No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT4630.25320332281509594No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGGACAATGGGCGAAAGCCCGAT4610.25210957196060313No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC4440.24281268969741382No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3840.21000016406262817No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTGGACAATGGGCGAAAGCCCGAT3820.2089064132081353No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT3540.19359390124523534No Hit
CCTACGGGGGGCAGCAGTCGGGAATTTTGCTCAATGGGCGAAAGCCTGAA3520.19250015039074247No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC3030.16570325445566755No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTGGACAATGGGCGAAAGCCCGAT2970.162422001892189No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT2880.15750012304697114No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2760.150937617920014No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTGGACAATGGGCGAAAGCCCGAT2670.14601573907479615No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT2640.14437511279305687No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT2590.14164073565682472No Hit
CCTACGGGGGGCTGCAGATGTGGGATCTGTGATGTCACAGGCTTCTCCCA2490.13617198138436046No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2340.12796884997566405No Hit
CCTACGGGGAGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT2320.12687509912117118No Hit
CCTACGGGGTGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC2290.1252344728394319No Hit
CCTACGGGGAGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2260.12359384655769262No Hit
CCTACGGGGAGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2220.12140634484870691No Hit
CCTACGGGGAGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC2210.12085946942146047No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2150.11757821685798192No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2120.11593759057624264No Hit
CCTACGGGTGGCAGCAGTCGGGAATTTTGCTCAATGGGCGAAAGCCTGAA2110.11539071514899621No Hit
CCTACGGGGGGCTGCAGTCCCAGTGTTCCAGCTCAGGACCAATGAGGTTG2020.11046883630377836No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1860.10171882946783553No Hit
CCTACGGGGTGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1860.10171882946783553No Hit
CCTACGGGAGGCAGCAGTCGGGAATTTTGCTCAATGGGCGAAAGCCTGAA1840.10062507861334266No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)