FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002458415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002458415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182857
Sequences flagged as poor quality0
Sequence length35-301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG96595.282269751773243No Hit
GACTACTGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG78124.272190837649092No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG56693.1002367970599978No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG54002.9531273071307087No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN48022.626095801637345No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG47472.5960176531387913No Hit
GACTACTCGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG45522.489376944825738No Hit
GACTACAGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG44432.4297675232558777No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG42532.3258611920790564No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG41752.283204908753835No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA40722.226876739747453No Hit
GACTACTAGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG38792.121329782288892No Hit
GACTACCGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG37552.0535172293103354No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG34281.8746889646007534No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG31561.7259388483897253No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG30921.690938821045954No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG30801.6843763159189968No Hit
GACTACACGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG28801.5750012304697112No Hit
GACTACCCGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG28001.531251196289997No Hit
GACTACCAGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG27331.4946105426644865No Hit
GACTACAAGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG25181.3770323258065047No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG24231.3250791602180938No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG23581.2895322574470762No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA22051.2058603170783726No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA21421.1714071651618478No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG21191.15882903033518No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA20481.1200008750006836No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG19791.08226647052068No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA16650.9105475863653019No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG15280.8356256528325412No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG14760.8071881306157271No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG14380.7864068643803629No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA13460.7360943250736914No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA13420.7339068233647057No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA13250.7246099411015166No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG13100.7164068096928201No Hit
GACTACTGGGGTATCTAATCCCGTTTGCTCCCCTAGCTTTCGCGCCTCAG12670.6928911663212237No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA11770.6436723778690453No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG10640.581875454590199No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA8260.4517191029055492No Hit
GACTACAGGGGTATCTAATCCCGTTTGCTCCCCTAGCTTTCGCGCCTCAG7700.4210940789797492No Hit
GACTACTCGGGTATCTAATCCCGTTTGCTCCCCTAGCTTTCGCGCCTCAG7350.40195343902612424No Hit
GACTACTAGGGTATCTAATCCCGTTTGCTCCCCTAGCTTTCGCGCCTCAG6760.36968778881858505No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG6290.3439846437380029No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG5920.32375025292988513No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG5690.31117211810321727No Hit
GACTACCGGGGTATCTAATCCCGTTTGCTCCCCTAGCTTTCGCGCCTCAG5620.3073439901124923No Hit
GACTACACGGGTATCTAATCCCGTTTGCTCCCCTAGCTTTCGCGCCTCAG5020.27453146447770665No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG4810.26304708050553166No Hit
GACTACCAGGGTATCTAATCCCGTTTGCTCCCCTAGCTTTCGCGCCTCAG4570.24992207025161742No Hit
GACTACCCGGGTATCTAATCCCGTTTGCTCCCCTAGCTTTCGCGCCTCAG4530.24773456854263165No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA4470.24445331597915312No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG4460.2439064405519067No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCACCTCAG4370.23898456170668883No Hit
GACTACAAGGGTATCTAATCCCGTTTGCTCCCCTAGCTTTCGCGCCTCAG4090.22367204974378888No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA4070.222578298889296No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA3920.2143751674805996No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG3920.2143751674805996No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG3830.20945328863538176No Hit
GACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3820.2089064132081353No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG3530.19304702581798894No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA3490.19085952410900323No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG3480.1903126486817568No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA3030.16570325445566755No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCACCTCAG2980.1629688773194354No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2880.15750012304697114No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCACCTCAG2870.1569532476197247No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2820.15421887048349256No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2770.15148449334726044No Hit
GACTACTGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCCCTCAG2420.13234385339363547No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG2400.13125010253914263No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCACCTCAG2380.13015635168464976No Hit
GACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2370.12960947625740332No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2320.12687509912117118No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2220.12140634484870691No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG2190.11976571856696762No Hit
GACTACTGGGGTATCTAATCCCATCTAAGCCTGAGTCCAGGTGCTTGGGA2160.11812509228522836No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCACCTCAG2150.11757821685798192No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2060.11265633801276406No Hit
GACTACAGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2050.11210946258551766No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCACCTCAG1920.10500008203131408No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCACCTCAG1900.10390633117682124No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG1870.10226570489508195No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG1860.10171882946783553No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)