FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002458416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002458416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198190
Sequences flagged as poor quality0
Sequence length35-301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC134796.801049497956506No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC123186.215247994348857No Hit
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC119736.041172612139866No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC117315.919067561430951No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC88254.452797820273475No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC79544.013320550986427No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC77133.891720066602755No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC68963.4794893788788537No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC40532.045007316211716No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC32431.636308592764519No Hit
CCTACGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC24941.2583884151571725No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC20251.0217468086179928No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC18190.9178061456178414No Hit
CCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC17610.8885412987537212No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC15570.7856097684040566No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT15480.7810686714768657No Hit
CCTACGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC15330.7735001765982138No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT14760.744739896059337No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC14140.7134567838942429No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC14130.7129522175689995No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC12790.6453403299863767No Hit
CCTACGGGGTGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC11010.5555275240930421No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT10960.5530046924668247No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC10520.5308037741561128No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC10140.5116302537968617No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT10120.5106211211463747No Hit
CCTACGGGGGGCTGCAGCTGCAGGAGGGTTTGGAGAAGAGGCTGCTGTGT9830.49598869771431453No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC9800.49447499873858414No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9490.4788334426560371No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC7970.40213936121903227No Hit
CCTACGGGTGGCTGCAGCTGCAGGAGGGTTTGGAGAAGAGGCTGCTGTGT7700.388516070437459No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC7440.3753973459811292No Hit
CCTACGGGGTGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC6840.34512336646652203No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC6240.31484938695191483No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT6080.3067763257480196No Hit
CCTACGGGAGGCTGCAGCTGCAGGAGGGTTTGGAGAAGAGGCTGCTGTGT5930.2992078308693678No Hit
CCTACGGGGGGCTGCAGGGATTAGATACCCCAGTAGTC5830.29416216761693326No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5760.2906302033402291No Hit
CCTACGGGCGGCTGCAGCTGCAGGAGGGTTTGGAGAAGAGGCTGCTGTGT5750.2901256370149856No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT5190.26186992280135224No Hit
CCTACGGGTGGCTGCAGGGATTAGATACCCCAGTAGTC4660.2351279075634492No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC4330.21847721883041524No Hit
CCTACGGGGGGCAGCAGCTGCAGGAGGGTTTGGAGAAGAGGCTGCTGTGT4330.21847721883041524No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCCAGTAGTC4320.2179726525051718No Hit
CCTACGGGGAGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3980.20081739744689442No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3930.19829456582067714No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3810.19223976991775568No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3790.19123063726726877No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3480.17558908118472172No Hit
CCTACGGGTGGCTGCAGGATTAGATACCCCAGTAGTC3360.16953428528180028No Hit
CCTACGGGGGGCTGCAGGGATTAGATACCCGAGTAGTC3340.1685251526313134No Hit
CCTACGGGCGGCTGCAGGGATTAGATACCCCAGTAGTC3300.16650688733033958No Hit
CCTACGGGGGGCTGCAGGGATTAGATACCCCTGTAGTC3110.15692012715071396No Hit
CCTACGGGAGGCTGCAGGGATTAGATACCCCAGTAGTC3060.15439729552449669No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2990.15086533124779253No Hit
CCTACGGGGGGCTGCAGGGATTAGATACCCTAGTAGTC2970.14985619859730562No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2940.14834249962157525No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC2680.13522377516524547No Hit
CCTACGGGGGGCTGCAGATGGATTAGATACCCCAGTAGTC2560.12916897926232404No Hit
CCTACGGGGAGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2500.1261415813108633No Hit
CCTACGGGGGGCTGCAGGGATTAGATACCCCGGTAGTC2490.12563701498561985No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2480.1251324486603764No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCCTGTAGTC2480.1251324486603764No Hit
CCTACGGGGGGCTGCCGGACCCGTCTCTGGTGGATCGTCCGATTGAGGGC2440.12311418335940259No Hit
CCTACGGGTGGCAGCAGCTGCAGGAGGGTTTGGAGAAGAGGCTGCTGTGT2420.1221050507089157No Hit
CCTACGGGGTGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2400.12109591805842877No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2360.11907765275745498No Hit
CCTACGGGCGGCTGCAGGATTAGATACCCCAGTAGTC2320.11705938745648116No Hit
CCTACGGGGGGCTGCAGGGATTAGATACCCGGGTAGTC2310.1165548211312377No Hit
CCTACGGGTGGCTGCAGGGATTAGATACCCCTGTAGTC2240.11302285685453352No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCTAGTAGTC2180.1099954589030728No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCGAGTAGTC2180.1099954589030728No Hit
CCTACGGGGGGCTGCAGAAACCAATGCCCCATGAAGGAGGTTGGGGGATA2160.1089863262525859No Hit
CCTACGGGTGGCTGCAGGGATTAGATACCCGAGTAGTC2150.10848175992734246No Hit
CCTACGGGGGGCTGCCGGACGCTAGGCAGGAAAACATTCCTGCAGATTCT2140.10797719360209898No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAT2120.10696806095161208No Hit
CCTACGGGAGGCTGCAGGATTAGATACCCCAGTAGTC2090.10545436197588172No Hit
CCTACGGGGGGCTGCAGGGATTAGATACCCTGGTAGTC2070.10444522932539482No Hit
CCTACGGGGGGCTGCAGGGGGTGGGGGATATCAAAAAGCTTTACTTGGGA2050.10343609667490793No Hit
CCTACGGTGGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2020.10192239769917756No Hit
CCTACGGGAGGCTGCAGGGATTAGATACCCGAGTAGTC2000.10091326504869065No Hit
CCTACGGGTGGCTGCAGGGATTAGATACCCTAGTAGTC1990.10040869872344721No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1990.10040869872344721No Hit
CCTACGGGCGGCTGCAGGGATTAGATACCCCTGTAGTC1990.10040869872344721No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1990.10040869872344721No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)