FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002458418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002458418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200624
Sequences flagged as poor quality0
Sequence length35-301
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGGGGAATCTTGCGCAATGGGCGGAAGCCTGAC71213.549425791530425No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT62093.094844086450275No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGCGGAAGCCTGAC60623.021572693197225No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT54682.725496451072653No Hit
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC54082.6955897599489593No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT53962.68960842172422No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT48822.4334077677645745No Hit
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC45942.289855650370843No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTGCGCAATGGGCGGAAGCCTGAC44362.211101363745115No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGCGGAAGCCTGAC42742.1303532977111415No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT41002.0436238934524282No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT36911.839759948959247No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT34581.7236222984289018No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC34391.714151846239732No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT33871.6882327139325304No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC31811.5855530744078477No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC24781.2351463434085652No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC22611.126983810511205No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC20391.016329053353537No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT19320.9629954541829492No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTGCGCAATGGGCGGAAGCCTGAC18930.9435561049525482No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC18420.9181354174974081No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC17830.8887271712257757No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT16890.8418733551319881No Hit
CCTACGGGGGGCTGCAGTCGAGGATCTTCGGCAATGCGGGCAACCGCGAC16330.8139604434165403No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC15750.7850506419969695No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC15730.7840537522928462No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT15730.7840537522928462No Hit
CCTACGGGTGGCTGCAGTCGAGGATCTTCGGCAATGCGGGCAACCGCGAC14680.7317170428263817No Hit
CCTACGGGGGGCTGCAGTAGGGAATTTTCCACAATGGACGAAAGTCTGAT14030.6993181274423799No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT13790.6873554509929022No Hit
CCTACGGGGGGCTGCAGTAGGGAATATTGGGCAATGGGCGAGAGCCTGAC13460.6709067708748704No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTGCGCAATGGGCGGAAGCCTGAC13040.6499720870882846No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC12520.624052954781083No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC12340.6150809474439748No Hit
CCTACGGGTGGCTGCAGTAGGGAATTTTCCACAATGGACGAAAGTCTGAT11920.594146263657389No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT11440.5702209107584337No Hit
CCTACGGGTGGCTGCAGTAGGGAATATTGGGCAATGGGCGAGAGCCTGAC11330.5647380173857565No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT11100.5532737857883403No Hit
CCTACGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC10680.5323391020017545No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT10070.5019339660259988No Hit
CCTACGGGAGGCTGCAGTCGAGGATCTTCGGCAATGCGGGCAACCGCGAC10040.5004386314698142No Hit
CCTACGGGCGGCTGCAGTCGAGGATCTTCGGCAATGCGGGCAACCGCGAC9990.4979464072095064No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT9980.49744796235744476No Hit
CCTACGGGCGGCTGCAGTAGGGAATTTTCCACAATGGACGAAAGTCTGAT9320.4645506021213813No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTGCGCAATGGGCGGAAGCCTGAC9160.45657548448839624No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT9100.4535848153760268No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT9020.4495972565595342No Hit
CCTACGGGAGGCTGCAGTAGGGAATTTTCCACAATGGACGAAAGTCTGAT8860.44162213892654917No Hit
CCTACGGGCGGCTGCAGTAGGGAATATTGGGCAATGGGCGAGAGCCTGAC8560.42666879336470215No Hit
CCTACGGGAGGCTGCAGTAGGGAATATTGGGCAATGGGCGAGAGCCTGAC8510.42417656910439433No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT8430.4201890102879018No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTGCGCAATGGGCGGAAGCCTGAC8300.41370922721110137No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT8270.41221389265491665No Hit
CCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC8210.4092232235425473No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT7890.39327298827657703No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT7490.3733351941941144No Hit
CCTACGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC6860.3419331685142356No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC6730.3354533854374352No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT6400.3190047053194035No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT6130.3055466943137411No Hit
CCTACGGGGTGCTGCAGTGGGGAATCTTGCGCAATGGGCGGAAGCCTGAC5630.28062445171066275No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC5560.2771353377462318No Hit
CCTACGGGGGGCAGCAGTCGAGGATCTTCGGCAATGCGGGCAACCGCGAC5490.27364622378180076No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT5470.27264933407767766No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC5430.27065555466943136No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC4470.22280484887152088No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT4440.22130951431533616No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT4410.21981417975915143No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC4400.2193157349070899No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC4110.2048608341973044No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT4110.2048608341973044No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3920.1953903820081346No Hit
CCTACGGGTGGCAGCAGTCGAGGATCTTCGGCAATGCGGGCAACCGCGAC3830.19090437833958052No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3620.18043703644628759No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3570.17794481218597974No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCCAGTAGTC3540.17644947762979504No Hit
CCTACGGGGGGCAGCAGTAGGGAATATTGGGCAATGGGCGAGAGCCTGAC3510.17495414307361035No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3480.17345880851742562No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3350.16697902544062526No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC3270.1629914666241327No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT3250.16199457692000957No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC3240.161496132067948No Hit
CCTACGGGGGGCAGCAGTAGGGAATTTTCCACAATGGACGAAAGTCTGAT3200.15950235265970175No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT3180.1585054629555786No Hit
CCTACGGGGGGAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATG3170.15800701810351703No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3120.15551479384320918No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2910.14504745194991625No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2880.14355211739373155No Hit
CCTACGGGGTGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2770.13806922402105432No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC2650.1320878857963155No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2650.1320878857963155No Hit
CCTACGGGAGGCAGCAGTCGAGGATCTTCGGCAATGCGGGCAACCGCGAC2630.13109099609219238No Hit
CCTACGGGCGGCAGCAGTCGAGGATCTTCGGCAATGCGGGCAACCGCGAC2600.12959566153600766No Hit
CCTACGGGTGGCTGCAGGATTAGATACCCCAGTAGTC2590.12909721668394608No Hit
CCTACGGGTGGCAGCAGTAGGGAATATTGGGCAATGGGCGAGAGCCTGAC2500.12461121301539198No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2470.12311587845920727No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC2450.12211898875508413No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC2410.12012520934683787No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT2330.11613765053034532No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2320.11563920567828376No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT2250.11215009171385278No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2210.11015631230560649No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2190.10915942260148338No Hit
CCTACGGGTGGCAGCAGTAGGGAATTTTCCACAATGGACGAAAGTCTGAT2140.10666719834117554No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCGAGTAGTC2140.10666719834117554No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2110.10517186378499081No Hit
CCTACGGGCGGCTGCAGGATTAGATACCCCAGTAGTC2040.10168274982055986No Hit
CCTACGGGGAGCTGCAGTGGGGAATCTTGCGCAATGGGCGGAAGCCTGAC2030.10118430496849828No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)