FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002458419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002458419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200624
Sequences flagged as poor quality0
Sequence length35-301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG70513.5145346518861156No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG55072.7449358003030544No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA50802.532099848472765No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG43512.1687335513198818No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG42462.1163968418534176No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG42142.100446606587447No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG36681.828295717361831No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG32971.6433726772469892No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG32491.619447324348034No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG31061.54816971050323No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA28281.409602041630114No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG28051.398137810032698No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA27551.3732155674296196No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG26441.3178881888507856No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG26071.2994457293245074No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG25951.2934643910997687No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG25861.2889783874312146No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA25721.2820001595023527No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG24751.2336510088523807No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG24021.1972645346518862No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG23781.1853018582024084No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA22191.1060491267246193No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG21881.0905973363107107No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG21481.0706595422282479No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG19630.9784472445968578No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG19440.968976792407688No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG19230.9585094505143952No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA18610.9276058696865779No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG18250.9096618550123614No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCAG17610.8777613844804211No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA17140.8543344764335273No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA17080.8513438073211579No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG16750.8348951272031262No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG16740.8343966823510647No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG16310.8129635537124172No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG15780.7865459765531541No Hit
GACTACTGGGGTATCTAATCCCTTTCGCTCCCCTAGCTTTCGTCCCTCAG15720.7835553074407848No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA15450.7700972964351225No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG15450.7700972964351225No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG14700.7327139325305049No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGCCTCAG11840.5901587048408964No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCACGCTTTCGTGCCTTAG11720.5841773666161576No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCAG10380.5173857564399075No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCAG10320.514395087327538No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG9960.49645107265332167No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCAG9440.4705319403461201No Hit
GACTACAGGGGTATCTAATCCCTTTCGCTCCCCTAGCTTTCGTCCCTCAG9380.4675412712337507No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG9230.4600645984528272No Hit
GACTACTCGGGTATCTAATCCCTTTCGCTCCCCTAGCTTTCGTCCCTCAG8830.44012680437036444No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG8780.4376345801100566No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG8700.4336470212935641No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG8170.40722944413430096No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG8080.4027434404657469No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCAG8030.400251216205439No Hit
GACTACTAGGGTATCTAATCCCTTTCGCTCCCCTAGCTTTCGTCCCTCAG7970.3972605470930696No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG7750.38629476034771515No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCACGCTTTCGTGCCTTAG7660.38180875667916103No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGCCTCAG7350.36635696626525244No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCACGCTTTCGTGCCTTAG7310.36436318685700614No Hit
GACTACACGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCAG7280.3628678523008214No Hit
GACTACCGGGGTATCTAATCCCTTTCGCTCCCCTAGCTTTCGTCCCTCAG7170.3573849589281442No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCAG6800.3389424994018662No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGCCTCAG6760.3369487199936199No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCACGCTTTCGTGCCTTAG6660.3319642714730042No Hit
GACTACTGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCCATGAG6450.3214969295797113No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG6260.3120264773905415No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTCCCCACGCTTTCGTGCCTTAG6180.30803891857404897No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCAG6180.30803891857404897No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG6150.3065435840178643No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGCCTCAG6060.3020575803493102No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG5950.29657468697663286No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCAG5890.29358401786426347No Hit
GACTACACGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG5830.2905933487518941No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG5710.2846120105271553No Hit
GACTACACGGGTATCTAATCCCTTTCGCTCCCCTAGCTTTCGTCCCTCAG5640.28112289656272427No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG5620.2801260068586012No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGCCTCAG5530.27564000319004706No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG5440.27115399952149294No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG5240.26118510248026156No Hit
GACTACCAGGGTATCTAATCCCTTTCGCTCCCCTAGCTTTCGTCCCTCAG5210.25968976792407683No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG5170.25769598851583064No Hit
GACTACAAGGGTATCTAATCCCTTTCGCTCCCCTAGCTTTCGTCCCTCAG5020.2502193157349071No Hit
GACTACCCGGGTATCTAATCCCTTTCGCTCCCCTAGCTTTCGTCCCTCAG5020.2502193157349071No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTCCCCACGCTTTCGTGCCTTAG5010.2497208708828455No Hit
GACTACACGGGTATCTAATCCCGTTCGCTCCCCACGCTTTCGTGCCTTAG4770.2377581944333679No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTCCCCACGCTTTCGTGCCTTAG4690.23377063561687536No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCAG4640.23127841135656751No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGCCTCAG4620.2302815216524444No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA4570.22778929739213655No Hit
GACTACTCGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCCATGAG4390.2188172900550283No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGCCTCAG4360.2173219554988436No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTCCCCACGCTTTCGTGCCTTAG4310.21482973123853577No Hit
GACTACAGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCCATGAG4280.21333439668235107No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGCCTCAG4270.21283595183028947No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4070.20286705478905812No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGCCTCAG3930.1958888268601962No Hit
GACTACTGGGGTATCTAATCCTGCAGCCCCCCGTAGG3880.19339660259988833No Hit
GACTACTAGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCCATGAG3640.1814339261504107No Hit
GACTACCGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCCATGAG3390.1689728048488715No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCAG3260.16249302177207114No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG3210.1600007975117633No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCAG3090.15401945928702448No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2740.13657388946486962No Hit
GACTACTGGGGTATCTAATCCTGCAGCCACCCGTAGG2730.13607544461280804No Hit
GACTACACGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCCATGAG2720.13557699976074647No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCAG2670.13308477550043862No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCAG2670.13308477550043862No Hit
GACTACCAGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCCATGAG2500.12461121301539198No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2490.12411276816333042No Hit
GACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2490.12411276816333042No Hit
GACTACTCGGGTATCTAATCCTGCAGCCCCCCGTAGG2440.12162054390302257No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2400.1196267644947763No Hit
GACTACAAGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCCATGAG2300.11464231597416062No Hit
GACTACTGGGGTATCTAATCCTGCAGCCTCCCGTAGG2300.11464231597416062No Hit
GACTACCCGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCCATGAG2210.11015631230560649No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2200.10965786745354494No Hit
GACTACTGGGGTATCTAATCCTGCAGCCGCCCGTAGG2180.1086609777494218No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2160.10766408804529866No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2150.1071656431932371No Hit
GACTACAGGGGTATCTAATCCTGCAGCCCCCCGTAGG2120.1056703086370524No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCAG2110.10517186378499081No Hit
GACTACTAGGGTATCTAATCCTGCAGCCCCCCGTAGG2010.10018741526437513No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)