FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002458420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002458420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162500
Sequences flagged as poor quality0
Sequence length35-301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC146339.004923076923077No Hit
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC122687.549538461538462No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC91185.611076923076923No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC84515.200615384615385No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN70804.356923076923077No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC44272.724307692307692No Hit
CCTACGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC25991.5993846153846154No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC19401.1938461538461538No Hit
CCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC18261.1236923076923078No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC16801.0338461538461539No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC16771.032No Hit
CCTACGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC16601.0215384615384615No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC16030.9864615384615385No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC15320.9427692307692307No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC12330.7587692307692308No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC11440.7040000000000001No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC11230.691076923076923No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC10880.6695384615384615No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC10000.6153846153846154No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT8890.547076923076923No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC8840.544No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT8720.5366153846153846No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC8410.5175384615384615No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC8360.5144615384615384No Hit
CCTACGGGGGGCTGCAGAAACCAATGCCCCATGAAGGAGGTTGGGGGATA7410.45599999999999996No Hit
CCTACGGGGTGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC7180.4418461538461539No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC6760.416No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC6490.39938461538461545No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT6390.3932307692307692No Hit
CCTACGGGGGGCTGCAAAGAAGGATTAGATACCCCAGTAGTC6240.384No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5980.368No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC5850.36No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC5840.35938461538461536No Hit
CCTACGGGTGGCTGCAGAAACCAATGCCCCATGAAGGAGGTTGGGGGATA5410.33292307692307693No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC4960.30523076923076925No Hit
CCTACGGGTGGCTGCAAAGAAGGATTAGATACCCCAGTAGTC4700.28923076923076924No Hit
CCTACGGGGGGCTGCAGGGGGTGGGGGATATCAAAAAGCTTTACTTGGGA4490.2763076923076923No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC4200.25846153846153846No Hit
CCTACGGGCGGCTGCAAAGAAGGATTAGATACCCCAGTAGTC3900.24No Hit
CCTACGGGTGGCTGCAGGGGGTGGGGGATATCAAAAAGCTTTACTTGGGA3900.24No Hit
CCTACGGGAGGCTGCAGAAACCAATGCCCCATGAAGGAGGTTGGGGGATA3830.23569230769230767No Hit
CCTACGGGCGGCTGCAGAAACCAATGCCCCATGAAGGAGGTTGGGGGATA3720.2289230769230769No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3690.22707692307692307No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3530.21723076923076923No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3510.216No Hit
CCTACGGGGGGCTGCAAAGAAGGATTAGATACCCCTGTAGTC3460.2129230769230769No Hit
CCTACGGGGGGCTGCAAAGAAGGATTAGATACCCGAGTAGTC3430.21107692307692308No Hit
CCTACGGGAGGCTGCAAAGAAGGATTAGATACCCCAGTAGTC3400.20923076923076925No Hit
CCTACGGGGGGCTGCAAAGAAGGATTAGATACCCTAGTAGTC3390.20861538461538462No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC3130.1926153846153846No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC3050.1876923076923077No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2930.1803076923076923No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2890.17784615384615385No Hit
CCTACGGGGGGCTGCAGGTCCAATGTCTGGTGAGGGCCCACTCCCTGGTT2630.16184615384615383No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC2600.16No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2570.15815384615384614No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2540.15630769230769231No Hit
CCTACGGGGGGCAGCAGAAACCAATGCCCCATGAAGGAGGTTGGGGGATA2520.15507692307692308No Hit
CCTACGGGGAGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2520.15507692307692308No Hit
CCTACGGGCGGCTGCAGGGGGTGGGGGATATCAAAAAGCTTTACTTGGGA2520.15507692307692308No Hit
CCTACGGGTGGCTGCAAAGAAGGATTAGATACCCCTGTAGTC2480.15261538461538462No Hit
CCTACGGGTGGCTGCAAAGAAGGATTAGATACCCGAGTAGTC2480.15261538461538462No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2470.152No Hit
CCTACGGGAGGCTGCAGGGGGTGGGGGATATCAAAAAGCTTTACTTGGGA2460.1513846153846154No Hit
CCTACGGGGGGCTGCAAAGAAGGATTAGATACCCCGGTAGTC2440.15015384615384614No Hit
CCTACGGGGGGCTGCAGTGAGCTGAGGTCACACCACTGCCCTTCAACCTG2380.14646153846153845No Hit
CCTACGGGTGGCTGCAAAGAAGGATTAGATACCCTAGTAGTC2360.14523076923076925No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCCAGTAGTC2340.14400000000000002No Hit
CCTACGGGGGGCTGCAAAGAAGGATTAGATACCCGTGTAGTC2280.1403076923076923No Hit
CCTACGGGCGGCTGCAAAGAAGGATTAGATACCCCTGTAGTC2280.1403076923076923No Hit
CCTACGGGGGGCTGCAAAGAAGGATTAGATACCCTGGTAGTC2260.13907692307692307No Hit
CCTACGGGGGGCTGCAAAGAAGGATTAGATACCCGGGTAGTC2180.13415384615384615No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2170.13353846153846155No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC2130.1310769230769231No Hit
CCTACGGGTGGCTGCAGTGAGCTGAGGTCACACCACTGCCCTTCAACCTG2120.13046153846153846No Hit
CCTACGGGTGGCTGCAAAGAAGGATTAGATACCCCGGTAGTC2100.12923076923076923No Hit
CCTACGGGCGGCTGCAAAGAAGGATTAGATACCCGAGTAGTC2090.1286153846153846No Hit
CCTACGGGGGGCTGCAAAGAAGGATTAGATACCCTTGTAGTC2090.1286153846153846No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2050.12615384615384614No Hit
CCTACGGGAGGCTGCAAAGAAGGATTAGATACCCCTGTAGTC2020.12430769230769231No Hit
CCTACGGGAGGCTGCAAAGAAGGATTAGATACCCGAGTAGTC1940.11938461538461538No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1900.11692307692307692No Hit
CCTACGGGGGGCTGCAGAGGATTAGATACCCCAGTAGTC1900.11692307692307692No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1820.11199999999999999No Hit
CCTACGGGAGGCTGCAAAGAAGGATTAGATACCCTAGTAGTC1800.11076923076923079No Hit
CCTACGGGGGGCTGCAGTGGATTAGATACCCCAGTAGTC1750.10769230769230768No Hit
CCTACGGGTGGCTGCAGGATTAGATACCCCAGTAGTC1740.10707692307692308No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC1730.10646153846153845No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC1720.10584615384615385No Hit
CCTACGGGTGGCTGCAGGTCCAATGTCTGGTGAGGGCCCACTCCCTGGTT1720.10584615384615385No Hit
CCTACGGGCGGCTGCAGGTCCAATGTCTGGTGAGGGCCCACTCCCTGGTT1720.10584615384615385No Hit
CCTACGGGTGGCTGCAAAGAAGGATTAGATACCCGTGTAGTC1710.10523076923076922No Hit
CCTACGGGGTGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC1700.10461538461538462No Hit
CCTACGGGCGGCTGCAAAGAAGGATTAGATACCCTAGTAGTC1650.10153846153846155No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)