FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002458421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002458421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162500
Sequences flagged as poor quality0
Sequence length35-301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA141558.71076923076923No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA80284.940307692307693No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA72384.4541538461538455No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN70774.355076923076924No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA66654.101538461538461No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA58363.591384615384616No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA47842.944No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA46902.8861538461538463No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA42932.641846153846154No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA42082.5895384615384613No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG17201.0584615384615386No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA15090.9286153846153846No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG9900.6092307692307692No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8980.5526153846153846No Hit
GACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA8930.5495384615384615No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA8530.5249230769230769No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8200.5046153846153846No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGTCTCAG8030.49415384615384617No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA7700.47384615384615386No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA7680.4726153846153846No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7370.45353846153846156No Hit
GACTACTGGGGTATCTAATCCTTCTTTGCAGCCCCCCGTAGG6980.4295384615384615No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA6750.41538461538461535No Hit
GACTACTGGGGTATCTAATCCAGTCCTCAACACAAGGCTCCGGGGCCTGA6040.3716923076923077No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5940.36553846153846153No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5930.3649230769230769No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5810.3575384615384615No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA5520.3396923076923077No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA5500.3384615384615385No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5320.3273846153846154No Hit
GACTACTGGGGTATCTAATCCTTCTTTGCAGCCACCCGTAGG5210.3206153846153846No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5080.31261538461538463No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGTCTCAG4830.29723076923076924No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA4620.2843076923076923No Hit
GACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4590.2824615384615385No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA4460.2744615384615384No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGTCTCAG4430.2726153846153846No Hit
GACTACAGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4310.2652307692307692No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGTCTCAG4200.25846153846153846No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG4150.2553846153846154No Hit
GACTACTGGGGTATCTAATCCTAAAACAACCAAAGTCTCGCCACCATGGC4100.2523076923076923No Hit
GACTACTGGGGTATCTAATCCTTCTTTGCAGCCGCCCGTAGG4090.25169230769230766No Hit
GACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4030.248No Hit
GACTACTCGGGTATCTAATCCAGTCCTCAACACAAGGCTCCGGGGCCTGA3940.24246153846153845No Hit
GACTACAGGGGTATCTAATCCTTCTTTGCAGCCCCCCGTAGG3890.2393846153846154No Hit
GACTACAGGGGTATCTAATCCAGTCCTCAACACAAGGCTCCGGGGCCTGA3770.232No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCTCCTCAG3770.232No Hit
GACTACTGGGGTATCTAATCCTTCTTTGCAGCCTCCCGTAGG3740.23015384615384615No Hit
GACTACTCGGGTATCTAATCCTTCTTTGCAGCCCCCCGTAGG3720.2289230769230769No Hit
GACTACCGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3400.20923076923076925No Hit
GACTACTAGGGTATCTAATCCTTCTTTGCAGCCCCCCGTAGG3390.20861538461538462No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGTCTCAG3240.19938461538461538No Hit
GACTACTAGGGTATCTAATCCAGTCCTCAACACAAGGCTCCGGGGCCTGA3220.19815384615384618No Hit
GACTACCCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3070.18892307692307692No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGTCTCAG3040.18707692307692309No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGTCTCAG2980.18338461538461537No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2910.17907692307692308No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGTCTCAG2780.17107692307692307No Hit
GACTACCGGGGTATCTAATCCAGTCCTCAACACAAGGCTCCGGGGCCTGA2720.16738461538461538No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2710.16676923076923078No Hit
GACTACTCGGGTATCTAATCCTTCTTTGCAGCCACCCGTAGG2690.16553846153846155No Hit
GACTACACGGGTATCTAATCCTTCTTTGCAGCCCCCCGTAGG2670.16430769230769232No Hit
GACTACCGGGGTATCTAATCCTTCTTTGCAGCCCCCCGTAGG2660.1636923076923077No Hit
GACTACTGGGGTATCTAATCCTGCAGCCCCCCGTAGG2620.16123076923076923No Hit
GACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2610.1606153846153846No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGTCTCAG2600.16No Hit
GACTACAGGGGTATCTAATCCTTCTTTGCAGCCACCCGTAGG2580.15876923076923077No Hit
GACTACCCGGGTATCTAATCCAGTCCTCAACACAAGGCTCCGGGGCCTGA2550.15692307692307692No Hit
GACTACACGGGTATCTAATCCAGTCCTCAACACAAGGCTCCGGGGCCTGA2530.15569230769230769No Hit
GACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2520.15507692307692308No Hit
GACTACCCGGGTATCTAATCCTTCTTTGCAGCCCCCCGTAGG2500.15384615384615385No Hit
GACTACTAGGGTATCTAATCCTTCTTTGCAGCCACCCGTAGG2490.15323076923076925No Hit
GACTACAGGGGTATCTAATCCTTCTTTGCAGCCGCCCGTAGG2450.15076923076923077No Hit
GACTACCAGGGTATCTAATCCTTCTTTGCAGCCCCCCGTAGG2450.15076923076923077No Hit
GACTACAGGGGTATCTAATCCTAAAACAACCAAAGTCTCGCCACCATGGC2410.1483076923076923No Hit
GACTACTGGGGTATCTAATCCTCAGTGGGATGGTATTTGGAGATGGGACT2400.14769230769230768No Hit
GACTACTCGGGTATCTAATCCTAAAACAACCAAAGTCTCGCCACCATGGC2370.14584615384615385No Hit
GACTACAAGGGTATCTAATCCTTCTTTGCAGCCCCCCGTAGG2340.14400000000000002No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2330.1433846153846154No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2320.1427692307692308No Hit
GACTACTGGGGTATCTAATCCACTGAACACATAAATGATAAGAAAGTAAA2320.1427692307692308No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCTCCTCAG2310.14215384615384616No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2290.14092307692307693No Hit
GACTACCGGGGTATCTAATCCTTCTTTGCAGCCACCCGTAGG2290.14092307692307693No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2280.1403076923076923No Hit
GACTACCAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2250.13846153846153844No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCTCCTCAG2240.13784615384615384No Hit
GACTACAGGGGTATCTAATCCTTCTTTGCAGCCTCCCGTAGG2220.1366153846153846No Hit
GACTACCAGGGTATCTAATCCAGTCCTCAACACAAGGCTCCGGGGCCTGA2210.136No Hit
GACTACAAGGGTATCTAATCCAGTCCTCAACACAAGGCTCCGGGGCCTGA2170.13353846153846155No Hit
GACTACTCGGGTATCTAATCCTTCTTTGCAGCCGCCCGTAGG2170.13353846153846155No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCTCCTCAG2110.12984615384615386No Hit
GACTACTGGGGTATCTAATCCTCTGCAGCCCCCCGTAGG2050.12615384615384614No Hit
GACTACTCGGGTATCTAATCCTTCTTTGCAGCCTCCCGTAGG2030.12492307692307691No Hit
GACTACTGGGGTATCTAATCCACTGCAGCCCCCCGTAGG2010.12369230769230768No Hit
GACTACTAGGGTATCTAATCCTAAAACAACCAAAGTCTCGCCACCATGGC1950.12No Hit
GACTACACGGGTATCTAATCCTTCTTTGCAGCCACCCGTAGG1940.11938461538461538No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1890.11630769230769232No Hit
GACTACTGGGGTATCTAATCCTGCAGCCACCCGTAGG1860.11446153846153845No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1850.11384615384615385No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1840.11323076923076922No Hit
GACTACTAGGGTATCTAATCCTTCTTTGCAGCCTCCCGTAGG1810.11138461538461539No Hit
GACTACCGGGGTATCTAATCCTAAAACAACCAAAGTCTCGCCACCATGGC1810.11138461538461539No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1770.10892307692307691No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1760.10830769230769231No Hit
GACTACCAGGGTATCTAATCCTAAAACAACCAAAGTCTCGCCACCATGGC1750.10769230769230768No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1740.10707692307692308No Hit
GACTACCAGGGTATCTAATCCTTCTTTGCAGCCACCCGTAGG1680.10338461538461538No Hit
GACTACTAGGGTATCTAATCCTTCTTTGCAGCCGCCCGTAGG1680.10338461538461538No Hit
GACTACAAGGGTATCTAATCCTTCTTTGCAGCCACCCGTAGG1670.10276923076923075No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)