FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002458422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002458422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239643
Sequences flagged as poor quality0
Sequence length35-301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2544210.616625563859575No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT234659.79164841034372No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT191998.011500440238189No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT166336.940741018932329No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT68732.868016174058913No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT48442.0213400766974208No Hit
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC46361.9345443013148726No Hit
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC42741.7834862691587068No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT36631.5285236789724714No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT35431.4784491931748476No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC32831.3699544739466623No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGACAATGGGTGGAAGCCTGAT32211.3440826562845567No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC28091.1721602550460477No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC27671.1546341850168793No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGACAATGGGTGGAAGCCTGAT26201.09329293991479No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT26041.08661634180844No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC23350.9743660361454329No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGACAATGGGTGGAAGCCTGAT19850.8283154525690297No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGACAATGGGTGGAAGCCTGAT19330.8066165087233926No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC18620.7769891046264652No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC16450.6864377428090952No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC14960.6242619229437121No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT13940.5816986100157318No Hit
CCTACGGGGGGCTGCAGTGAGTGCTGGGGTCAGCCCCATCGTATAGAGAT13020.54330817090422No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT12760.5324586989814015No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12670.5287031125465796No Hit
CCTACGGGGAGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT12580.5249475261117579No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGACAATGGGTGGAAGCCTGAT10550.44023818763744405No Hit
CCTACGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC9920.41394908259369145No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT9900.4131145078303977No Hit
CCTACGGGTGGCTGCAGTGAGTGCTGGGGTCAGCCCCATCGTATAGAGAT9460.3947538630379356No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT8780.3663783210859487No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC7910.3300743188826713No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT7600.31713841005161847No Hit
CCTACGGGGTGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT7540.31463468576173725No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT7270.30336792645727184No Hit
CCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC7230.3016987769306844No Hit
CCTACGGGCGGCTGCAGTGAGTGCTGGGGTCAGCCCCATCGTATAGAGAT6840.28542456904645663No Hit
CCTACGGGAGGCTGCAGTGAGTGCTGGGGTCAGCCCCATCGTATAGAGAT6770.28250355737492855No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGACAATGGGTGGAAGCCTGAT6740.28125169522998794No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC6340.2645601999641133No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGTCGAAAGCCTGAT5900.24619955517165118No Hit
CCTACGGTGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT5790.24160939397353562No Hit
CCTACGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC5600.2336809337222452No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGACAATGGGTGGAAGCCTGAT5290.22074502489119233No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGTCGAAAGCCTGAT5160.21532028892978305No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC5080.21198198987660813No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC5050.2107301277316675No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT4980.20780911606013944No Hit
CCTACGGGGGGCTGCAGTGGGGAATTGTTCGCAATGGGCGCAAGCCTGAC4810.20071523057214272No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT4590.19153490817591165No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGTCGAAAGCCTGAT4450.18569288483285554No Hit
CCTACGGGGCGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT4410.18402373530626806No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT4390.18318916054297435No Hit
CCTACGGGTGGCTGCAGTGGGGAATTGTTCGCAATGGGCGCAAGCCTGAC4270.17818171196321195No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC4120.17192240123850897No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC4070.16983596433027462No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGGCGAAAGCCTGAT3950.16482851575051222No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGACAATGGGTGGAAGCCTGAT3920.1635766536055716No Hit
CCTACGGGGAGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3880.16190750407898416No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3810.15898649240745608No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGTCGAAAGCCTGAT3640.15189260691945938No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3630.1514753195378125No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3540.1477197331029907No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT3530.14730244572134385No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGGCGAAAGCCTGAT3430.14312957190487516No Hit
CCTACGGGGGGCAGCAGTGAGTGCTGGGGTCAGCCCCATCGTATAGAGAT3340.13937398547005336No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCATGAT3240.13520111165358473No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT3080.12852451354723485No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCATGAT3040.12685536402064737No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2890.12059605329594437No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT2830.11809232900606319No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGGCGAAAGCCTGAT2800.1168404668611226No Hit
CCTACGGGAGGCTGCAGTGGGGAATTGTTCGCAATGGGCGCAAGCCTGAC2680.1118330182813602No Hit
CCTACGGGCGGCTGCAGTGGGGAATTGTTCGCAATGGGCGCAAGCCTGAC2580.10766014446489153No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCATGAT2510.10473913279336346No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2450.10223540850348226No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)