FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002458427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002458427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193363
Sequences flagged as poor quality0
Sequence length35-301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG145547.526776063672988No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG84654.377776513603947No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG82684.275895595331061No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG73103.780454378552257No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG69483.593241726700558No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA68103.5218733677073693No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG54872.837668013011797No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG50352.603910779208018No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG50032.587361594513945No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG45812.3691192213608603No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA40152.0764055170844475No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA37951.962629872312697No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA35851.854025847757844No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN34681.7935178912201404No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG34301.773865734395929No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA33111.7123234538148457No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA26511.3709965194995941No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA25341.3104885629618903No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA24301.2567037127061536No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA21961.1356877996307464No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG20461.05811349637728No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG18330.947957985757358No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG17910.9262371808463874No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG17310.8952074595450008No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA16830.8703836825038916No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG14090.7286812885608933No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG14010.724543992387375No Hit
GACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA12270.6345578006133542No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG12190.6304205044398359No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG10830.5600864694900265No Hit
GACTACTGGGGTATCTAATCCCAGTCAACTGTCCTCATGGCCTAATCGCC10440.5399171506441253No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA9890.5114732394511876No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA9640.49854418890894325No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA8610.44527650067489644No Hit
GACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA7920.40959232117830197No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA7580.3920088124408496No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA6960.35994476709608353No Hit
GACTACAGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA6690.3459813925104596No Hit
GACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA6410.33150085590314593No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA6400.33098369388145615No Hit
GACTACCGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA6400.33098369388145615No Hit
GACTACTGGGGTATCTAATCCTCTGCCCCTAGGGAGTGGGGGACGCTTCT6390.3304665318597663No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA6370.32943220781638677No Hit
GACTACTCGGGTATCTAATCCCAGTCAACTGTCCTCATGGCCTAATCGCC6050.312883023122314No Hit
GACTACAGGGGTATCTAATCCCAGTCAACTGTCCTCATGGCCTAATCGCC5880.30409126875358783No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA5640.2916793802330332No Hit
GACTACTAGGGTATCTAATCCCAGTCAACTGTCCTCATGGCCTAATCGCC5320.2751301955389604No Hit
GACTACCGGGGTATCTAATCCCAGTCAACTGTCCTCATGGCCTAATCGCC5240.2709928993654422No Hit
GACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA5010.2590981728665774No Hit
GACTACCAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4740.24513479828095341No Hit
GACTACCCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4580.23686020593391705No Hit
GACTACACGGGTATCTAATCCCAGTCAACTGTCCTCATGGCCTAATCGCC4060.20996778080604872No Hit
GACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3960.20479616058915098No Hit
GACTACCAGGGTATCTAATCCCAGTCAACTGTCCTCATGGCCTAATCGCC3940.2037618365457714No Hit
GACTACCCGGGTATCTAATCCCAGTCAACTGTCCTCATGGCCTAATCGCC3930.20324467452408163No Hit
GACTACTCGGGTATCTAATCCTCTGCCCCTAGGGAGTGGGGGACGCTTCT3930.20324467452408163No Hit
GACTACTGGGGTATCTAATCCAGCCTGGAGGTTAATATGCGGCTTTACAC3930.20324467452408163No Hit
GACTACAGGGGTATCTAATCCTCTGCCCCTAGGGAGTGGGGGACGCTTCT3560.18410967972155998No Hit
GACTACAAGGGTATCTAATCCCAGTCAACTGTCCTCATGGCCTAATCGCC3360.17376643928776445No Hit
GACTACTAGGGTATCTAATCCTCTGCCCCTAGGGAGTGGGGGACGCTTCT3350.1732492772660747No Hit
GACTACCGGGGTATCTAATCCTCTGCCCCTAGGGAGTGGGGGACGCTTCT3320.17169779120100537No Hit
GACTACTGGGGTATCTAATCCAGGCAAGACTTGTCAGCGCTGGGACCAGCAGCCCCCCGTAGG3240.16756049502748716No Hit
GACTACTGGGGTATCTAATCCAGCAGGCACCCAGCCAACTCAATGCAATG3150.1629060368322792No Hit
GACTACTGGGGTATCTAATCCCTGGACTTGATGAATGTTACCAAATTAAG2840.14687401415989615No Hit
GACTACTGGGGTATCTAATCCCAGAATGACAAGTATCAAATGACAAAAAA2520.13032482946582336No Hit
GACTACACGGGTATCTAATCCTCTGCCCCTAGGGAGTGGGGGACGCTTCT2490.12877334340075403No Hit
GACTACCCGGGTATCTAATCCTCTGCCCCTAGGGAGTGGGGGACGCTTCT2450.12670469531399492No Hit
GACTACTGGGGTATCTAATCCAGGCAAGACTTGTCAGCGCTGGGACCAGCAGCCACCCGTAGG2430.12567037127061537No Hit
GACTACTAGGGTATCTAATCCAGCCTGGAGGTTAATATGCGGCTTTACAC2380.1230845611621665No Hit
GACTACCGGGGTATCTAATCCAGCCTGGAGGTTAATATGCGGCTTTACAC2100.10860402455485278No Hit
GACTACCAGGGTATCTAATCCTCTGCCCCTAGGGAGTGGGGGACGCTTCT2080.10756970051147324No Hit
GACTACTCGGGTATCTAATCCAGCAGGCACCCAGCCAACTCAATGCAATG2030.10498389040302436No Hit
GACTACTCGGGTATCTAATCCAGCCTGGAGGTTAATATGCGGCTTTACAC1960.10136375625119592No Hit
GACTACTAGGGTATCTAATCCAGCAGGCACCCAGCCAACTCAATGCAATG1940.10032943220781639No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)