FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002458432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002458432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences271788
Sequences flagged as poor quality0
Sequence length35-301
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC148795.474487468173724No Hit
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC126764.663929238965665No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC101883.7485098679853417No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC88273.247751924293935No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN85683.152457062121948No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA74442.7388994363253714No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT67962.5004783139800137No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT61182.25101917671126No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA58552.1542525792161538No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC52411.9283412071173118No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC49001.8028757708213754No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT45521.6748347977099798No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT45011.6560701723402065No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA44671.6435604220936908No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA41601.5306047360442698No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC34771.279305929621617No Hit
CCTACGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC34301.2620130395749627No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC27671.0180729097679073No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC27160.9993082843981338No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC26720.9831191958438195No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA25310.9312405257038574No Hit
CCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC24940.917626973965002No Hit
CCTACGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC22820.8396250018396691No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC20910.7693496401607135No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT18880.6946590725123993No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC16730.6155532988947268No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA16360.6019397471558715No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC15250.5610990919393056No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC15140.557051819800727No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG13980.5143714954302618No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA13670.5029655466760857No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT12830.47205910489057645No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA11260.4142934934581365No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC10190.37492457356469017No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG10010.3683017646106524No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT9870.36315069097973424No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC9490.34916920541009905No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT9080.3340839183481243No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCCAGTAGTC8250.3035454103933949No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC7830.2880921895006402No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC7740.28478078502362136No Hit
CCTACGGGGTGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC7610.27999764522348297No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG7530.277054174577244No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG7410.27263896860788556No Hit
CCTACGGGGGGCTGCAGTGAGGAATCTTCCACAATGGGCGAAAGCCTGAT7200.2649123581615082No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT6060.22296790145260276No Hit
CCTACGGGTGGCTGCAGGATTAGATACCCCAGTAGTC5920.21781682782168457No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC5850.21524129100622547No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC5770.21229782035998646No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC5680.20898641588296762No Hit
CCTACGGGTGGCTGCAGTGAGGAATCTTCCACAATGGGCGAAAGCCTGAT5660.20825054822140787No Hit
CCTACGGGGGGCTGCAGGGACATCCTTTACTGTTGACTGAAATGGTGATG5590.20567501140594877No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG5280.19426906265177268No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC5050.18580658454383564No Hit
CCTACGGGGGGCTGCAGATTAATGGCAGATTCTAAAGCACAGCAGTCCCC5010.18433484922071613No Hit
CCTACGGGGTGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA5010.18433484922071613No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCGAGTAGTC4840.17807997409745832No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC4770.1755044372819992No Hit
CCTACGGGCGGCTGCAGGATTAGATACCCCAGTAGTC4700.1729289004665401No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCCTGTAGTC4630.170353363651081No Hit
CCTACGGGCGGCTGCAGTGAGGAATCTTCCACAATGGGCGAAAGCCTGAT4380.16115501788158418No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCTAGTAGTC4380.16115501788158418No Hit
CCTACGGGAGGCTGCAGTGAGGAATCTTCCACAATGGGCGAAAGCCTGAT4360.16041915022002443No Hit
CCTACGGGAGGCTGCAGGATTAGATACCCCAGTAGTC4160.15306047360442698No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC4120.15158873828130748No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC4030.14827733380428865No Hit
CCTACGGGTGGCTGCAGGGACATCCTTTACTGTTGACTGAAATGGTGATG4000.14717353231194902No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC3990.14680559848116914No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC3890.14312626017337043No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC3810.14018278952713142No Hit
CCTACGGGTGGCTGCAGATTAATGGCAGATTCTAAAGCACAGCAGTCCCC3760.13834312037323207No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC3740.13760725271167235No Hit
CCTACGGGGGGCTGCAGTGATAAATTGTTAGCAATAAACGAAAGTTTAAG3710.13650345121933272No Hit
CCTACGGGGGGCTGCCCCAGAATCAGCAAAAAGTAGCCTTCATGGTATGT3710.13650345121933272No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCCGGTAGTC3300.12141816415735794No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCGTGTAGTC3240.1192105611726787No Hit
CCTACGGGTGGCTGCCCCAGAATCAGCAAAAAGTAGCCTTCATGGTATGT3160.11626709052643973No Hit
CCTACGGGTGGCTGCAGGATTAGATACCCGAGTAGTC3150.11589915669565985No Hit
CCTACGGGTGGCTGCAGGATTAGATACCCCTGTAGTC3140.11553122286487999No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCTGGTAGTC3070.11295568604942087No Hit
CCTACGGGCGGCTGCAGGGACATCCTTTACTGTTGACTGAAATGGTGATG3040.11185188455708125No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG3020.11111601689552152No Hit
CCTACGGGTGGCTGCAGGATTAGATACCCTAGTAGTC2920.10743667858772278No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCTTGTAGTC2870.10559700943382343No Hit
CCTACGGGGAGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2830.10412527411070394No Hit
CCTACGGGCGGCTGCAGATTAATGGCAGATTCTAAAGCACAGCAGTCCCC2810.1033894064491442No Hit
CCTACGGGGGGCTGCAGAAGCGAGGAGGATTAGATACCCCAGTAGTC2780.10228560495680458No Hit
CCTACGGGGTGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2780.10228560495680458No Hit
CCTACGGGTGGCTGCAGTGATAAATTGTTAGCAATAAACGAAAGTTTAAG2740.10081386963368508No Hit
CCTACGGGGGGCTGCAGAAACCAATGCCCCATGAAGGAGGTTGGGGGATA2740.10081386963368508No Hit
CCTACGGGAGGCTGCAGGGACATCCTTTACTGTTGACTGAAATGGTGATG2740.10081386963368508No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCGGGTAGTC2730.1004459358029052No Hit
CCTACGGGCGGCTGCAGGATTAGATACCCGAGTAGTC2730.1004459358029052No Hit
CCTACGGGCGGCTGCCCCAGAATCAGCAAAAAGTAGCCTTCATGGTATGT2730.1004459358029052No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)