FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002458433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002458433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences271788
Sequences flagged as poor quality0
Sequence length35-301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA162995.996953507881143No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA94833.4891165172855314No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA91473.365490750143494No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN85613.1498815253064887No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA83723.080342031289093No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA70342.5880465657056235No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG70202.582895492074705No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA58022.1347520861848204No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA57022.0979587031068334No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA56142.0655805259982047No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA51871.9084727802551988No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG42161.5512090305679427No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG40711.4978586251048611No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG38961.4334702047183834No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG37901.3944692186557168No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA37091.3646665783625473No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG27241.0022517550443728No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGCTCCTCAG26740.9838550635053792No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG25870.9518448202275303No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG24180.8896640028257318No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG23870.8782580540715558No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG23310.857653759547883No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG22850.8407288033320087No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG22750.8370494650242101No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA21360.7859066625458078No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA20750.7634626988682355No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG20310.7472736103139211No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA18900.6953949401739591No Hit
GACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA17810.6552901526189531No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG17630.6486673436649153No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGCTCCTCAG16370.6023076809866513No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGCTCCTCAG14700.5408627312464127No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG14600.537183392938614No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA14550.5353437237847145No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG14310.5265133118459976No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG14280.525409510353658No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGCTCCTCAG14150.5206263705535197No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA13240.48714439195255127No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA13210.48604059046021164No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA12830.47205910489057645No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG12450.45807761932094127No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA11960.4400488616127276No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGCTCCTCAG10820.3981044049038221No Hit
GACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA10370.38154738251872783No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGCTCCTCAG9940.3657262277951933No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGCTCCTCAG9880.3635186248105141No Hit
GACTACAGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA9680.35615994819491664No Hit
GACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA9680.35615994819491664No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGCTCCTCAG9060.33334805068656453No Hit
GACTACTGGGGTATCTAATCCTGCAGCCCCCCGTAGG9050.33298011685578466No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGCTCCTCAG9040.3326121830250048No Hit
GACTACCGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA7450.27411070393100506No Hit
GACTACTGGGGTATCTAATCCGGTTCGCTCCCCACGCTTTCGTGCCTTAG6720.24725153428407434No Hit
GACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA6690.24614773279173474No Hit
GACTACCCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA6650.24467599746861524No Hit
GACTACTGGGGTATCTAATCCTGCAGCCACCCGTAGG6190.2277510412527411No Hit
GACTACCAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA6120.225175504437282No Hit
GACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA6050.22259996762182288No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5880.21634509249856504No Hit
GACTACTCGGGTATCTAATCCTGCAGCCCCCCGTAGG5220.19206145966709345No Hit
GACTACAGGGGTATCTAATCCTGCAGCCCCCCGTAGG5180.19058972434397398No Hit
GACTACTGGGGTATCTAATCCTGCAGCCGCCCGTAGG5000.1839669153899363No Hit
GACTACTGGGGTATCTAATCCAAAGGATGAGGAGGATAGAGGAGGGCAAG4860.17881584175901805No Hit
GACTACTAGGGTATCTAATCCTGCAGCCCCCCGTAGG4740.17440063578965959No Hit
GACTACTGGGGTATCTAATCCTGCAGCCTCCCGTAGG4500.16557022385094264No Hit
GACTACTGGGGTATCTAATCCCTGGGCCATGGACATGTACTGGTCTGTGG4280.15747567957378547No Hit
GACTACTGGGGTATCTAATCCTCATTACACATACCATGAAGGCTACTTTT3990.14680559848116914No Hit
GACTACAGGGGTATCTAATCCGGTTCGCTCCCCACGCTTTCGTGCCTTAG3820.14055072335791133No Hit
GACTACACGGGTATCTAATCCTGCAGCCCCCCGTAGG3590.13208824524997426No Hit
GACTACCGGGGTATCTAATCCTGCAGCCCCCCGTAGG3540.13024857609607488No Hit
GACTACAGGGGTATCTAATCCTGCAGCCACCCGTAGG3440.12656923778827617No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3410.12546543629593654No Hit
GACTACTCGGGTATCTAATCCTGCAGCCACCCGTAGG3360.12362576714203717No Hit
GACTACCAGGGTATCTAATCCTGCAGCCCCCCGTAGG3350.12325783331125731No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3330.12252196564969756No Hit
GACTACTCGGGTATCTAATCCGGTTCGCTCCCCACGCTTTCGTGCCTTAG3250.11957849500345857No Hit
GACTACTGGGGTATCTAATCCCAGTTAGGGTCTTAGCTATTGCGTCTTGA3220.11847469351111896No Hit
GACTACAAGGGTATCTAATCCTGCAGCCCCCCGTAGG3180.11700295818799947No Hit
GACTACTAGGGTATCTAATCCTGCAGCCACCCGTAGG3170.1166350243572196No Hit
GACTACTGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG3120.11479535520332024No Hit
GACTACCCGGGTATCTAATCCTGCAGCCCCCCGTAGG3110.11442742137254037No Hit
GACTACTAGGGTATCTAATCCGGTTCGCTCCCCACGCTTTCGTGCCTTAG3030.11148395072630138No Hit
GACTACTGGGGTATCTAATCCTCCTCGCTTCTGCAGCCCCCCGTAGG3010.11074808306474164No Hit
GACTACTCGGGTATCTAATCCTGCAGCCGCCCGTAGG2940.10817254624928252No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2830.10412527411070394No Hit
GACTACTCGGGTATCTAATCCTGCAGCCTCCCGTAGG2800.10302147261836432No Hit
GACTACAGGGGTATCTAATCCTGCAGCCGCCCGTAGG2790.10265353878758443No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)