FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002458434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002458434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences395734
Sequences flagged as poor quality0
Sequence length35-301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC393929.954161128434755No Hit
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC354008.945402720008895No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC282867.147730546276033No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC250266.323944872060526No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC139253.5187777648622562No Hit
CCTACGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC92032.325552012210222No Hit
CCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC67191.6978576518570554No Hit
CCTACGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC63151.5957688750524341No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN60301.5237508023066No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT31770.8028119898719847No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAAGCCTGAA30930.7815856105363703No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT28010.7077986728459015No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT27530.6956693132255505No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT26420.6676201691034886No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAAGCCTGAA24590.6213769855509003No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCCAGTAGTC22070.5576978475440574No Hit
CCTACGGGGTGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC20470.5172666488095539No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT20380.514992393880738No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT20200.5104438840231065No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT19940.5038738142287497No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT19860.5018522542920244No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAAGCCTGAA18730.47329772018578137No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAAGCCTGAA17560.44373240611117565No Hit
CCTACGGGTGGCTGCAGGATTAGATACCCCAGTAGTC15920.4022904274083096No Hit
CCTACGGGCGGCTGCAGGATTAGATACCCCAGTAGTC13530.341896324298645No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCCTGTAGTC12830.3242076748522998No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC12630.3191537750104868No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCGAGTAGTC12510.3161214351053991No Hit
CCTACGGGAGGCTGCAGGATTAGATACCCCAGTAGTC12150.3070244153901358No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC11220.28352378112570564No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCTAGTAGTC11110.2807441362127085No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT10680.26987825155281076No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC9580.24208180242283958No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT9390.23728059757311729No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGACGCAAGTCTGAT9200.23247939272339502No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCCGGTAGTC9170.23172130774712307No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC9100.22995244280248853No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT9060.22894166283412595No Hit
CCTACGGGTGGCTGCAGGATTAGATACCCCTGTAGTC8980.22692010289740078No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT8960.22641471291321946No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGACAATGGGCGCAAGCCTGAT8660.21883386315050007No Hit
CCTACGGGTGGCTGCAGGATTAGATACCCGAGTAGTC8650.21858116815840944No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAAGCCTGAA8590.21706499820586556No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGACGCAAGTCTGAT8560.2163069132295936No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT8400.21226379335614326No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT8140.20569372356178647No Hit
CCTACGGGTGGCTGCAGGATTAGATACCCTAGTAGTC8130.2054410285696958No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCTGGTAGTC7920.20013443373579223No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCGTGTAGTC7680.1940697539256167No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCGGGTAGTC7400.1869942941470786No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGACAATGGGCGCAAGCCTGAT7370.18623620917080666No Hit
CCTACGGGCGGCTGCAGGATTAGATACCCGAGTAGTC7310.18472003921826277No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC7280.18396195424199083No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCTTGTAGTC7260.18345656425780954No Hit
CCTACGGGGGGCAGCAGGATTAGATACCCCAGTAGTC7230.1826984792815376No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC7150.18067691934481242No Hit
CCTACGGGGAGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC7040.1778972744318153No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC7040.1778972744318153No Hit
CCTACGGGGGGCTGCAGTAGAGAATCTTCCGCAATGGGCGAAAGCCTGAC7030.17764457943972464No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC6960.17587571449509015No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT6940.17537032451090886No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT6870.17360145956627432No Hit
CCTACGGGAGGCTGCAGGATTAGATACCCGAGTAGTC6790.17157989962954914No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGCACAATGGAGGAAACTCTGAT6710.16955833969282397No Hit
CCTACGGGAGGCTGCAGGATTAGATACCCCTGTAGTC6680.16880025471655202No Hit
CCTACGGGCGGCTGCAGGATTAGATACCCCTGTAGTC6670.1685475597244614No Hit
CCTACGGGTGGCTGCAGGATTAGATACCCCGGTAGTC6580.16627330479564556No Hit
CCTACGGGCGGCTGCAGGATTAGATACCCTAGTAGTC6440.1627355749063765No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGCACAATGGAGGAAACTCTGAT6370.16096670996174198No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT6310.1594505400091981No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT6290.1589451500250168No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT6250.15793437005665423No Hit
CCTACGGGAGGCTGCAGGATTAGATACCCTAGTAGTC6180.1561655051120197No Hit
CCTACGGGGTGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC6170.15591281011992905No Hit
CCTACGGGTGGCTGCAGTAGAGAATCTTCCGCAATGGGCGAAAGCCTGAC5990.15136430026229739No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC5970.1508589102781161No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGACGCAAGTCTGAT5930.14984813030975352No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT5820.1470684853967564No Hit
CCTACGGGTGGCTGCAGGATTAGATACCCGTGTAGTC5790.14631040042048446No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGACGCAAGTCTGAT5700.14403614549166863No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT5640.14251997553912477No Hit
CCTACGGGTGGCTGCAGGATTAGATACCCGGGTAGTC5630.14226728054703414No Hit
CCTACGGGTGGCTGCAGGATTAGATACCCTGGTAGTC5470.13822416067358378No Hit
CCTACGGGCGGCTGCAGGATTAGATACCCCGGTAGTC5460.13797146568149313No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT5430.13721338070522118No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAAGCCTGAA5430.13721338070522118No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5340.13493912577640538No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGACAATGGGCGCAAGCCTGAT5260.13291756583968017No Hit
CCTACGGGTGGCTGCAGGATTAGATACCCTTGTAGTC5130.12963253094250177No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC5060.12786366599786725No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGCACAATGGAGGAAACTCTGAT5030.12710558102159533No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGACAATGGGCGCAAGCCTGAT5010.12660019103741402No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT4960.12533671607696079No Hit
CCTACGGGAGGCTGCAGGATTAGATACCCCGGTAGTC4890.12356785113232627No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGCACAATGGAGGAAACTCTGAT4870.12306246114814497No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT4700.11876664628260396No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT4670.11800856130633204No Hit
CCTACGGGCGGCTGCAGGATTAGATACCCTGGTAGTC4570.11548161138542556No Hit
CCTACGGGAGGCTGCAGGATTAGATACCCGTGTAGTC4540.11472352640915363No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGTCGCAAGCCTGAA4520.11421813642497232No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAAGCCTGAA4520.11421813642497232No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC4430.11194388149615651No Hit
CCTACGGGCGGCTGCAGGATTAGATACCCTTGTAGTC4420.11169118650406586No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT4380.11068040653570328No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT4340.1096696265673407No Hit
CCTACGGGCGGCTGCAGGATTAGATACCCGTGTAGTC4330.10941693157525004No Hit
CCTACGGGGGGCTGCAGAAGCGGGGATTAGATACCCCAGTAGTC4310.10891154159106875No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAAGCCTGAA4260.1076480666306155No Hit
CCTACGGGGGGCAGCAGGATTAGATACCCCTGTAGTC4230.10688998165434359No Hit
CCTACGGGCGGCTGCAGGATTAGATACCCGGGTAGTC4230.10688998165434359No Hit
CCTACGGGCGGCTGCAGTAGAGAATCTTCCGCAATGGGCGAAAGCCTGAC4220.10663728666225293No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGTCGCAAGCCTGAA4040.10208877680462128No Hit
CCTACGGGAGGCTGCAGGATTAGATACCCTGGTAGTC4030.10183608181253065No Hit
CCTACGGGGGGCTGCAGTAGGGAATATTGCGCAATGGGGGAAACCCTGAC3990.10082530184416805No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)